Báo cáo khoa học: "The 23S rRNA gene PCR-RFLP used for characterization of porcine intestinal spirochete isolates" pptx

Ngày tải lên: 07/08/2014, 18:21

... transcriptional regulation of both the isc and the suf operons. In its holoform, IscR represses the isc operon transcription, whereas, in its apoform, it acts as an activator of the suf operon [43,44]. ... One microgram of RNA was used in each RT-PCR experiment. Samples were collected at the exponential phase of the Table 2. Sequences of the primers used in the RT-PCR experiment...

Ngày tải lên: 18/02/2014, 04:20

... the PCR prod- uct. For each run, serial dilutions of human GAPDH plas- mids were used as standards for quantitative measurement of the amount of amplified cDNA. For normalization of each sample, ... PCR was performed using 5 lLofa60lL DNA extraction in TE buffer with Hi-Fi Taq polymerase (Invitrogen) for 28 cycles (95 °C for 30 s, 52 °C for 30 s and 68 °C for 1 min). Th...

Ngày tải lên: 19/02/2014, 17:20

... bristles of the progeny were scored for Sb expression. On the wild-type genetic background, 29.7% of bristles showed the Sb phenotype. As a posi- tive control, we confirmed that on the background of Characterization ... R, Kondo S, Motoyama J & Higashinakagawa T (1995) Gene trap capture of a novel mouse gene, jumonji, requi- red for neural tube formation. Genes Dev 9, 1211...

Ngày tải lên: 23/03/2014, 07:20

... expression of AP-2e increases, we used an in vitro model system for HMSC differentiation into chondro- cytes established in our laboratory. Marker genes for different stages of chondrogenesis, ... samples used for immunohistology are of human origin. Cartilage was obtained from patients giving informed consent following the standards of the Ethics Com- mission of the Unive...

Ngày tải lên: 18/02/2014, 08:20

... solicit English translations of Urdu sentences from non-professional translators, and show that translation quality can rival that of professionals, for a fraction of the cost. Lei and Hansen ... the written form of the language, Modern Standard Arabic (MSA), differs in a non-trivial fashion from the various spo- ken varieties of Arabic. As the variant of choice for written an...

Ngày tải lên: 20/02/2014, 04:20

... responsible for binding of the XPC– hHR23B complex to sites of DNA damage, hHR23B stimulates XPC to function in NER and is also necessary for XPA–RPA-mediated displacement of the Fig. 1. Scheme of the ... responsible for the 3¢ and 5¢ DNA inci- sions, respectively. Binding of XPG induces the release of XPC–hHR23B, whereas XPF–ERCC1 triggers exci- sion of the damaged DNA an...

Ngày tải lên: 07/03/2014, 12:20

... Department of Biochemistry and Molecular Biology, School of Biomedical and Chemical Science, The University of Western Australia, Crawley, Australia The predominant source of the main form of vitamin ... novel pathway for the metabolism of secoster- oids. According to kinetic data generated by Guryev et al. [18] the rate (V max ) for P450scc processing vita- min D3 is 43% of...

Ngày tải lên: 07/03/2014, 21:20

... independent of genetic background (Fig. S5), and indicate that BACE1 is responsible for the generation of at least  20% of APLP2s. BACE1 manipulation alters the quantity and form of APP, APLP1 ... levels of soluble APP remaining constant irrespective of the presence or absence of BACE1. The levels of APPsa increased to account for the loss of APPsb (soluble C-terminally...

Ngày tải lên: 15/03/2014, 10:20

... synthetic toxin gene fused to the 3¢-end of the gene for glutathione S-transferase, with an intervening thrombin cleavage site. Engineered plasmids were trans- formed into E. coli BL21 cells for protein ... Overlay of the structure of J-ACTX-Hv1c (red) and ChTx (Protein Data Bank code 2CRD, blue). (G) Stereoview of an overlay of the functional dyad of ChTx (green side chai...

Ngày tải lên: 16/03/2014, 06:20