Báo cáo khoa học: "The 23S rRNA gene PCR-RFLP used for characterization of porcine intestinal spirochete isolates" pptx
Tài liệu Báo cáo khoa học: The alr2505 (osiS) gene from Anabaena sp. strain PCC7120 encodes a cysteine desulfurase induced by oxidative stress docx
... transcriptional
regulation of both the isc and the suf operons. In its
holoform, IscR represses the isc operon transcription,
whereas, in its apoform, it acts as an activator of the
suf operon [43,44]. ... One
microgram of RNA was used in each RT-PCR experiment.
Samples were collected at the exponential phase of the
Table 2. Sequences of the primers used in the RT-PCR experiment...
Tài liệu Báo cáo khoa học: The Ets transcription factor ESE-1 mediates induction of the COX-2 gene by LPS in monocytes doc
... the PCR prod-
uct. For each run, serial dilutions of human GAPDH plas-
mids were used as standards for quantitative measurement
of the amount of amplified cDNA. For normalization of
each sample, ... PCR was performed using
5 lLofa60lL DNA extraction in TE buffer with Hi-Fi
Taq polymerase (Invitrogen) for 28 cycles (95 °C for 30 s,
52 °C for 30 s and 68 °C for 1 min). Th...
Báo cáo khoa học: The Drosophila jumonji gene encodes a JmjC-containing nuclear protein that is required for metamorphosis pot
... bristles of the progeny were scored for
Sb expression. On the wild-type genetic background,
29.7% of bristles showed the Sb phenotype. As a posi-
tive control, we confirmed that on the background of
Characterization ... R,
Kondo S, Motoyama J & Higashinakagawa T (1995)
Gene trap capture of a novel mouse gene, jumonji, requi-
red for neural tube formation. Genes Dev 9, 1211...
Tài liệu Báo cáo khoa học: The cartilage-specific transcription factor Sox9 regulates AP-2e expression in chondrocytes pptx
... expression of AP-2e increases, we used an in vitro
model system for HMSC differentiation into chondro-
cytes established in our laboratory. Marker genes for
different stages of chondrogenesis, ... samples used for immunohistology are of
human origin. Cartilage was obtained from patients giving
informed consent following the standards of the Ethics Com-
mission of the Unive...
Tài liệu Báo cáo khoa học: "The Arabic Online Commentary Dataset: an Annotated Dataset of Informal Arabic with High Dialectal Content" pdf
... solicit English translations of
Urdu sentences from non-professional translators,
and show that translation quality can rival that of
professionals, for a fraction of the cost.
Lei and Hansen ... the written form
of the language, Modern Standard Arabic (MSA),
differs in a non-trivial fashion from the various spo-
ken varieties of Arabic. As the variant of choice for
written an...
Báo cáo khoa học: The protein shuffle Sequential interactions among components of the human nucleotide excision repair pathway pdf
... responsible for binding of the XPC–
hHR23B complex to sites of DNA damage, hHR23B
stimulates XPC to function in NER and is also
necessary for XPA–RPA-mediated displacement of the
Fig. 1. Scheme of the ... responsible for the 3¢ and 5¢ DNA inci-
sions, respectively. Binding of XPG induces the release
of XPC–hHR23B, whereas XPF–ERCC1 triggers exci-
sion of the damaged DNA an...
Báo cáo khoa học: The cytochrome P450scc system opens an alternate pathway of vitamin D3 metabolism docx
... Department of Biochemistry and Molecular Biology, School of Biomedical and Chemical Science, The University of Western Australia,
Crawley, Australia
The predominant source of the main form of
vitamin ... novel pathway for the metabolism of secoster-
oids. According to kinetic data generated by Guryev
et al. [18] the rate (V
max
) for P450scc processing vita-
min D3 is 43% of...
Báo cáo khoa học: b-Secretase cleavage is not required for generation of the intracellular C-terminal domain of the amyloid precursor family of proteins pot
... independent of
genetic background (Fig. S5), and indicate that BACE1
is responsible for the generation of at least 20% of
APLP2s.
BACE1 manipulation alters the quantity and form
of APP, APLP1 ... levels of soluble APP remaining constant
irrespective of the presence or absence of BACE1. The
levels of APPsa increased to account for the loss of
APPsb (soluble C-terminally...
Báo cáo khoa học: The Janus-faced atracotoxins are specific blockers of invertebrate KCa channels ppt
... synthetic toxin gene fused to the 3¢-end of the
gene for glutathione S-transferase, with an intervening
thrombin cleavage site. Engineered plasmids were trans-
formed into E. coli BL21 cells for protein ... Overlay of the structure of J-ACTX-Hv1c (red) and ChTx (Protein Data Bank code 2CRD,
blue). (G) Stereoview of an overlay of the functional dyad of ChTx (green side chai...
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