... lML-ascorbate (except for PL–), and 30 mM Hepes at 23 °C. PL+ and PL) represent the presence and absence, respectively, of L-ascorbate inthe reaction. Lanes M I and M II were loaded with 5¢-32P-labeled ... DNAzyme with RNA substrate and RNase A. Such regulatorybiocatalyst systems offer several advantages, includingthe ease of RNA substrate and DNAzyme synthesiswithout any chemical modification, and ... Hg2+[12,13], Pb2+[14], and UO22+[15]; and constructingmolecular logic gates and nanomotors [16–20]. How-ever, engineering an allosteric DNAzyme with dualRNA-cleaving and DNA-cleaving activities...