Báo cáo khoa học: " Induction of castration by immunization of male dogs with recombinant gonadotropin-releasing hormone (GnRH)-canine distemper virus (CDV) T helper cell epitope p35" pot

... k cat value, which expresses the acylation rate. By contrast, the decrease in the DK9 K m value is consistent with better accommodation of the substrate into the catalytic pocket of the unprotonated ... of the catalytic activity and stabil- ity of the DK9 mutant in comparison with WT throm- bin, using experimental techniques in conjunction with computational approaches to prov...

Ngày tải lên: 07/03/2014, 12:20

... neighbouring potential tags. ~otential word tag assignment. In cases where more than one potential tag is assigned to the inpu~ word, the tags represent word classes of the word without taking the ... according to initial capital letter status need to be solved if the system is to become completely automatic and capable of correct tagging of standard text. Constituent ;alalysi...

Ngày tải lên: 08/03/2014, 18:20

... than that of TEM-1 ( 28 000 Da) with the exception of TEM260–STa ( 28 000 Da) (data not shown). The N-terminal sequence of TEM260–STa was that of the wild-type TEM-1 (HPETL), suggesting that the ... similar. This indi- cates that the active site was not significantly altered by the insertion of the enterotoxin at position 195. The catalytic activity of the other hybrid protei...

Ngày tải lên: 30/03/2014, 02:20

... of the false morel mushroom, Gyromitra esculenta [4]. The ra- tionale behind the latter treatment is that the active toxin monomethylhydrazine competitively inhibits a pyridox- ine-dependent ... funiculi of L 4 showed a normal histopathologic pattern. Although this subcutaneous administration meth- od did not cause systemic toxicity and effectively induced sensory neuropathy, this st...

Ngày tải lên: 07/08/2014, 20:23

... Vet. Sci. (2005), / 6 (1), 21–24 Induction of castration by immunization of male dogs with recombinant gonadotropin-releasing hormone (GnRH)-canine distemper virus (CDV) T helper cell epitope ... ACT GCT GCT CAG ATC ACT GCT GGT ATC GCT CTA CAT CAG TCA AAT CTA AAT GAG CTC TGA GTC GAC-3', was synthesized (Bionex, Korea) and then the single-stran...

Ngày tải lên: 07/08/2014, 18:21

... shown that the sterol response element binding protein activates transcription of the rat SR-BI promoter in a variety of cell lines [14] and that steroidogenic factor 1 binds to and activates the ... was verified by sequencing (data not shown). Moreover, the mutant promoter construct with the point mutations (G T at position )336; G–C at position )330) was also per- formed using the...

Ngày tải lên: 18/02/2014, 04:20

... actin, leading to a very rapid dissolution of the actin cytoskeleton [29]. Pre- treatment with CytD as well as simultaneous treatment with Decma and CytD at concentrations known to dis- rupt the ... results suggest that it is not the absence of E-cadherin-dependent cell cell interaction per se but the very process of disruption of cell cell interaction that induces the signali...

Ngày tải lên: 19/02/2014, 05:20

... 5¢—AGAGGGA AATCGTGCGTGAC—3¢; actin reverse, 5¢—CAATAGTG ATGACCTGGCCGT—3¢; PPARb forward, 5¢—GTCGCA CAACGCTATCC—3¢; PPARb reverse, 5¢—CTCCGGGCC TTCTTTTTGGTCA—3¢; cPLA2 forward, 5¢—CATAAGT TTACTGTTGTGGTTCTA—3¢; ... activation of Raf. We therefore investigated whether 4-OHT treatment of N-BxB-ER cells would lead to an activation of the transcriptional activity of PPARb. To address this q...

Ngày tải lên: 07/03/2014, 12:20

... that the aromatic residue of the peptide inserts into the bilayer with all of the lipid mixtures, as indicated by the fact that most of the protons of the phospholipid show cross-peaks with the ... addition to electrostatic interactions with PtdIns(4, 5)P2, the NAP-22-peptide has two features that can contribute to its interaction with membranes. These features include membr...

Ngày tải lên: 07/03/2014, 17:20

... protein binding to the 5¢-UTR and 3¢-UTR. This indicates that RNA-BPs may contribute to post-transcriptional control of TCTP mRNA stability. Interestingly, the crosslinking patterns of cobalt- ... vector pGL3 promoter modified with TCTP UTRs. The Luc UTRs have been replaced by TCTP mRNA 5¢- and 3¢-UTRs (5¢⁄3¢), by TCTP mRNA 5¢-UTR (5¢), or by TCTP mRNA 3¢- UTR. The TCTP 3¢-UTR but...

Ngày tải lên: 16/03/2014, 05:20