Báo cáo khoa học: "Isolation and identification of Escherichia coli O157:H7 using different detection methods and molecular determination by multiplex PCR and RAPD" docx

... (2005), / 6 (1), 7–19 Isolation and identification of Escherichia coli O157:H7 using different detection methods and molecular determination by multiplex PCR and RAPD Ji-Yeon Kim 1,2 , So-Hyun ... were applied for further determination by multiplex PCR and PCR for flagellar H7 antigen detection. DNA preparation for Multiplex PCR, flagellar H...

Ngày tải lên: 07/08/2014, 18:21

... were analyzed by autoradiography and silver staining. Equal mobilities of the radioactive and the protein-containing bands indicated covalent attachment of DNA to the enzyme. The yield of the photochemical ... action of base release by Escherichia coli Fpg protein: role of lysine 155 in cata- lysis. Biochemistry 36, 5084–5096. 21. Smith, R.A.G. & Knowles, J.R. (1975)...

Ngày tải lên: 20/02/2014, 11:20

... Government of India, and MHRD, Government of India, for financial support. We also thank Professor Winfried Boos, University of Konstanz, Germany, for the gift of plasmids encoding His-tagged MalZ and ... refolding of Escherichia coli maltodextrin glucosidase Subhankar Paul 1,2 , Shashikala Punam 1 and Tapan K. Chaudhuri 1 1 Department of Biochemical Engineering and Bio...

Ngày tải lên: 16/03/2014, 06:20

... of rates and yields of interchromophore (folate fi flavin) energy-transfer and intermolecular (flavin fi DNA) electron-transfer in Escherichia coli photolyase by time-resolved fluorescence and absorption-spectroscopy. ... bands at 1532 and 1396 cm )1 , and changes in the amide-I (1600–1700 cm )1 ) region. The frequencies of infrared bands can be modulated by isotope substit...

Ngày tải lên: 16/03/2014, 18:20

... showed a correlation between C-1¢ of the xylosyl moiety and H-6 of the fructosyl moiety, and the other showed a corre- lation between C-1¢ of the xylosyl moiety and H-3 of the fructosyl moiety in the ... catalytic domain of each monomer is a triose-phosphate isomerase barrel flanked by two b-sandwich structures. The glycone-binding site is formed by the C-terminal ends of...

Ngày tải lên: 23/03/2014, 07:20

... Physico-Chemical Biology and School of Chemistry, Moscow State University, Moscow, Russia; 2 Department of Biochemistry, University of Turku, Finland We report here that Escherichia coli pyrophosphatase aggregates ... ligands – Asn24 and Asp26 – from two subunits Fig. 1. Time-course of Escherichia coli inorganic pyrophosphatase (PPase) aggregation in the presence of 2 m...

Ngày tải lên: 23/03/2014, 13:20

... 399 of one chain and Lys406 of another fibrin. Successively, the enzyme sta- bilizes the blood clot by cross-linking lysine and gluta- mine of the a and c chains of fibrin molecules [24]. Sobel and ... cross-linking of proteins mediated by TG isoforms results in well-defined functions in various biological processes: cross-linking of fibrin in haemostasis, of semenogelin...

Ngày tải lên: 30/03/2014, 15:20

... 100 lm of (GlcNAc) 1–4 was analysed at the start, in the middle and at the end of each series of sam- ples. The resulting average values of the standards (display- ing standard deviations of < ... Materials and methods) . (D) Chromatogram of (GlcNAc) 6 degradation products generated by LlChi18 after 2 min of incubation with 1 n M of enzyme. The double peaks represent...

Ngày tải lên: 18/02/2014, 08:20

... varies between 2 and 12 (Table 6). There is one completely conserved salt bridge between the C-terminal Glu (or Asp) of b-strand B6 (BAX and BCX have Asp) and Arg of the loop between b-strands B7 and A6. ... al.[8],theshapeofthemoleculeresemblesa right hand: two b-sheets and the a-helix form fingers and a palm, a long loop between the B7 and B8 strands forms a thumb, and a...

Ngày tải lên: 20/02/2014, 23:20

... proteolysis of the 55-kDa Tm-mas and PO activity by using two kinds of serine proteinase inhibitors, such as p-NPGB and p-APMSF. As shown in Fig. 7C and D, when HW-55S Fig. 6. Localization of the ... concentra- tions were determined by the method of Lowry et al.[16] using BSA as a standard. Molecular cloning of 45-kDa Tm-mas A cDNA library from T. molitor larvae was...

Ngày tải lên: 21/02/2014, 03:20