Báo cáo khoa học: "Two-dimensional gel electrophoresis and immunoblot analysis of Neospora caninum tachyzoites" ppt

... kVh. Two-dimensional gel electrophoresis and its immunoblot analysis of Neospora caninum tachyzoites 145 dimensional electrophoresis and mass spectrometry: proteomics of Toxoplasma gondii . ... 4-7 IPG strips. Two-dimensional gel electrophoresis and its immunoblot analysis of Neospora caninum tachyzoites 141 immunization) and incomplete adjuvant (secon...

Ngày tải lên: 07/08/2014, 17:23

... and spoken documents, es- pecially in the meeting domain, for example, the pres- ence of disfluencies and multiple speakers, and the lack of structure in spontaneous utterances. The question of whether ... those of the authors and do not necessarily reflect the views of NSF. References J. Carbonell and J. Goldstein. 1998. The use of mmr, diversity- based reranking for reor...

Ngày tải lên: 17/03/2014, 02:20

... expression and purification of recombinant proteins HAKN1 homeodomain and C-terminal sequences were amplified and cloned in-frame into the EcoRI and PstI sites of the expression vector pMAL-c2 (New England ... incorporation of Ile50 and Arg55 must have been particularly important. Other homeodomains that contain Ile50 and Arg55 are those of the TGIF, Meis and Bell families...

Ngày tải lên: 23/03/2014, 13:20

... cofactors are generally employed by enzymes to enable a wide and diverse range of biochemical transformations necessary for all aspects of life. Some of these cofactors, such as vitamin B 12 and ... N3 of a histidine) or to the 6-position (via the thiol group of a cysteine) of the isoalloxazine ring [44], or, in one case, it is bicovalently linked to N1 of a histidine and...

Ngày tải lên: 28/03/2014, 22:21

... in a total volume of 100 lL, comprising 2 lLof25mm dNTP mixture, 2 lLof20lm primer solution, 1 U of DeepVent DNA polymerase (New England Biolabs, Hitchin, England) and 100 ng of the tem- plate ... characterization of the ckMT, ackMT and bckMT metal complexes Spectroscopic (UV-Vis) and spectropolarimetric (CD) analy- sis of the metal–ckMT clusters and of the species formed...

Ngày tải lên: 30/03/2014, 11:20

... the structures of PSs from E. coli and M. tuberculosis. Structural analyses Analysis of the sites of substrate binding from NMR data, and structural comparison of the E. coli, M. tuberculosis and A. ... 164° and )169° in the A-chain and B- chain of nPS, respectively. The v 1 torsion angle for Asp35 changes from ) 75.20° to )171.65° and )89.25° in the A-chain and B-ch...

Ngày tải lên: 16/02/2014, 09:20

... 7.0 and 4 °C for 6 h, followed by SDS ⁄ PAGE. The rate of ligation was estimated from percentage intensity of the band of the 57-kDa protein in a total of intensities of the 57-kDa, 38-kDa and ... method of Siegel [30] was used to determine pK a values from the logk cat vs. pH and logk cat ⁄ K m vs. pH profiles. Analysis of the ligation process of the E78H/D164N mutant...

Ngày tải lên: 19/02/2014, 07:20

... AER: An Extensive Analysis of Word Alignments and Their Impact on MT Necip Fazil Ayan and Bonnie J. Dorr Institute of Advanced Computer Studies (UMIACS) University of Maryland College Park, MD ... S A and S B are actually used in decoding. In order to investigate this, we did an analysis of the phrases used to de- code the same test set. The bottom row of Figure 3 shows th...

Ngày tải lên: 20/02/2014, 11:21

... set of heads as import and a set of top categories as export. For an elaboration of the notion control expression and a formalisation of subgrammars we refer to section 6. The advantages of ... ce = [ce, l and ((D~ = D and u = t and A = false) or (D2, u, true) • CF_,-PARSER(GI, ce,, D, t) and A = true) } U { (D~, u, A) I B ce,: ce = {ce,} and ((D~ = D an...

Ngày tải lên: 22/02/2014, 10:20

... C, D and E are labeled as ssC, ssD, and ssE, respectively. The CD and DE loops are marked as lCD and lDE; B, b-bulge. Residue 53 is marked as X. (C) Stereo representation of strands D and E and ... state. Because the inser- tion of a proline breaks the regularity of a b-strand as a result of restrictions of the / and w angles, the resulting (partial) loss of secondar...

Ngày tải lên: 06/03/2014, 00:20