Báo cáo khoa học: "Biophysical characterization of Japanese encephalitis virus (KV1899) isolated from pigs in Korea" pps

... unit *5-iodo-deoxyuridine. **Log TCID 50 /ml in Vero cells. ***Mouse brain antigen. Biophysical characterization of Japanese encephalitis virus (KV1899) isolated from pig in Korea 127 fluid using RNA isolation ... purified from brain homogenate of infected mice by ultracentrifugation. The purified JE virus from brain homogenate using ultracentrifuge revealed homo...

Ngày tải lên: 07/08/2014, 17:22

... Molecular characterization of Japanese encephalitis virus in Korea 201 mosquito pool in Korea in 1994 (98.5% nucleotide identity). Because Anyang strain is the current vaccine strain for swine, we ... Korea We have determined the complete nucleotide and deduced amino acid sequences of the Japanese encephalitis virus (JEV) strain KV1899, isolated from a fattening pi...

Ngày tải lên: 07/08/2014, 17:23

Ngày tải lên: 07/08/2014, 20:23

... (1999) Vaccination with cathepsin L proteinases and with leucine aminopeptidase induces high levels of protection against fascioliasis in sheep. Infect Immun 67, 1954–1961. 3 Williamson AL, Brindley ... activity was defined as lmol of leucine, alanine or tyrosine released per min per mg of protein. The kinetic parameters k cat and K m were obtained from Hanes– Woolf plots. To examine...

Ngày tải lên: 16/03/2014, 10:20

... Viral wasting syndrome of swine: Experimental reproduction of postweaning multi- systemic wasting syndrome in gnotobiotic swine by coinfection with porcine circovirus 2 and porcine parvovirus. Vet. ... Pigletwastingdisease.Vet.Rec.1997,141, 660. 26.LyooY.S.,KimJ.H.,ParkC.K.Identification of Genetic characterization of porcine circovirus-2 field isolates from PMWS pigs 39 porci...

Ngày tải lên: 07/08/2014, 15:20

... the binding of RNA-binding proteins to 3¢ UTR tran- scripts has been poorly described in plants. PUF proteins are a large family of RNA-binding proteins found in all eukaryotes. These proteins ... FEBS Binding of APUM to NRE To test the predictions regarding the RNA-binding specificities of the putative APUM proteins, we investi- gated the capacity of APUM to bind to the NRE sequ...

Ngày tải lên: 18/02/2014, 06:20

... concentration of the 16 kDa protein subunit) increased linearly with increasing fractional saturation of the metal site in Bxe_A2876, the k cat determined from the molar concentration of Fe 2+ -containing ... a tetrad of three histidines and one glutamate was likewise seen in other members of the cupin protein superfamily, including oxalate oxidase (germin) [6]. Interestingly,...

Ngày tải lên: 18/02/2014, 06:20

... much in terms of the rate of the over- all reaction, as the cleavage reaction is not the limiting step (binding is slower), but in terms of decreasing the amount of substrate that dissociates from ... step of TES [48], similar to (if not the same as) the role of the xG in the second step of self-splicing [48–56]. This suggests that the guanosine to be excised is likely b...

Ngày tải lên: 18/02/2014, 18:20

... gradual diminution of the Soret band. After several minutes, a broad band appears at around 660–675 nm; this increases in inten- sity with time, indicating the formation of biliverdin. In this case, ... (a-1 in Fig. 3), indicating that in- plane anisotropy of heme is relatively large. In addition, small amounts of low-spin species are also observed in the neutral solution,...

Ngày tải lên: 19/02/2014, 05:20

... fitted using a nonlinear least squares routine using a single-site binding model in Origin for ITC version 5.0 (Microcal), varying the stoichiometry (n), binding constant (K b ), and binding enthalpy ... buried predominantly because of intermolecular interactions (binding) and not because of intramolecular interactions (folding). Small chan- ges in the thermodynamic parameters on foldi...

Ngày tải lên: 19/02/2014, 05:20