Báo cáo khoa học: "Escherichia coli O157:H7 adherence to HEp-2 cells is implicated with curli expression and outer membrane integrity" potx

... (E and F of Fig. 1). Autoaggregation test We alternatively examined degree of curliation of the CR+ Escherichia coli O157:H7 adherence to HEp-2 cells is implicated with curli expression and outer ... E and F). -2851$/ 2) 9H W H U L Q D U \  6FLHQFH J. Vet. Sci. (2004), / 5 (2), 119–124 Escherichia coli O157:H7 adherence to HEp-2 cells is implica...

Ngày tải lên: 07/08/2014, 17:22

... The yield of this purification is 28%, and the purification fold is 5.5. This simple protocol is fast enough (a few hours in total) to keep this labile enzyme active. The enzyme was stored best in ... E. coli enzyme, and it is not possible at t he moment to attribute the residue t hat is responsible for the inactivation. Furthermore, this chemical inactivation does not seem...

Ngày tải lên: 07/03/2014, 16:20

... the isomerization of the cis-isomer to the trans-isomer is not thought to be the function of the CyPA and CyPB proteins in these cells. On the other hand, CyPs facilitate the step of the isomerization in ... the N atom of Pro is directly isomerized to the cis-isomer with the sp 2 - hybrid configuration (Fig. 8B). On the other hand, in the in vitro system with CyP proteins, the...

Ngày tải lên: 30/03/2014, 15:20

... were filled with binding buffer, and the microplate was put to float upside down for 1 h in NaCl/P i solution to remove unbound cells. The bound cells (or the total amount of added cells without washing) ... binding of cells to BSA was substracted. The data is presented as a percentage of attached cells. The amounts of bound and added red cells were quantitated by counti...

Ngày tải lên: 20/02/2014, 11:20

... iden- tical to those of the unstimulated cells. This view is consistent with a previous report showing that MDP, but not a safe analogue such as MB, is capable of inducing mitochondrial proton leak ... consisting of a late phase response to MDP. At this stage, 1 to 2 h after MDP stimulation, oxida- tive stress has been induced and there is a need to counteract the toxic eff...

Ngày tải lên: 05/03/2014, 23:20

... MUC5AC, MUC6, and MUC19) have been described, and their expression appears to be tissue-specific [2–7]. In the normal situation, MUC5AC is primarily expressed in the airways and stomach, and it constitutes ... Their expressions are tissue-specific, but in disease states such as cys- tic fibrosis, inflammation and cancer, this specificity can be disturbed. MUC5AC is normally expre...

Ngày tải lên: 07/03/2014, 05:20

... the mapping with the ontology. This map- ping between words and ontology is generally to be built by hand. Most of the time in transport- ing the lexicon is spent in identifying and build- ... field label to the synonym Cer- tificate, to the 13 direct hyponyms and to the 27 1 Actually the problem is related to both polysemy and omonymy. As WordNet does not dist...

Ngày tải lên: 08/03/2014, 21:20

... SKD1 E235Q . HeLa cells were co- transfected with pMyc–SKD1 E235Q and either with pmGFP–Brox WT (A–C) or with pmGFP–Brox C408S (D–F). After 24 h, cells were fixed and stained with mAb to c-Myc and Cy3-labeled ... blocking with 0.1% (w ⁄ v) gela- tin in NaCl ⁄ P i for 30 min, the cells were incubated with primary antibodies (mAb to Strep-tag II, pAb to FLAG, mAb t...

Ngày tải lên: 16/03/2014, 06:20

... is able to stimulate PTEN expression and to induce apoptosis in acute lymphoblas- tic leukemia cells [27]. To evaluate the contribution of 5- Aza to the regulation of PTEN in gastric cancer cells, we ... control siRNA were treated with VD3 and 5-Aza, and apoptosis was evaluated by flow cytometry. The apoptosis rates were calculated on the basis of 30 000 cells (B). HG...

Ngày tải lên: 22/03/2014, 21:20

... FXII and PPK/HK may start with a local increase in the concentration of Zn 2+ as a result of leakage from disturbed cells. This leakage may lead to binding of FXII and PKK/HK to respective receptors ... second mechanism assumes that the inactivator binds to FXIIa, while still bound to the cell surface (Fig. 3B). To analyse which of these mechnisms was followed, the cells we...

Ngày tải lên: 23/03/2014, 20:22