Báo cáo khoa học: "Expression of apxIA of Actinobacillus pleuropneumoniae in Saccharomyces cerevisiae" docx

... identified in A. pleuropneumoniae , including a family of secreted toxins, or Apx toxins, which are members of the RTX (Repeat in ToXins) toxin family. Importance of Apx toxins in A. pleuropneumoniae ... words: Actinobacillus pleuropneumoniae , apx IA, expression, Saccharomyces cerevisiae Introduction Porcine pleuropneumoniae caused by Actinobacillus pleuropneum...

Ngày tải lên: 07/08/2014, 17:22

... FEBS NF-jBp65, indicated by strong immunostaining in the nucleus. Pretreatment with BAY 11-7082 inhibited hypoxia ⁄ SD-induced NF-jBp65 translocation, with substantial levels of NF-jBp65 staining remaining ... route [28]. Processing of pro-IL-1b into its active form AB CD Fig. 8. MSC-secreted IL-10 is involved in the inhibition of cardiac fibrosis. (A) BrdU incorporation in cardiac...

Ngày tải lên: 18/02/2014, 04:20

... (PX) domain in the N-terminus. PX domains occur in a variety of proteins involved in cell signaling, mem- brane trafficking, and polarity establishment, and func- tion as phosphoinositide-binding modules ... in the testis and fetal brain. The Noxo1c protein contains an additional five amino acids in the N-terminal PX domain, a phosphoinosi- tide-binding module; the domain plays an e...

Ngày tải lên: 19/02/2014, 06:20

... purified enzyme was incubated with different ratio of E600. In each case, the kinetics of inactivation of Rv1399c have been monitored b y measuring the remaining activity, a s a function of time, using tripropionin ... LB medium after 4 h of IPTG induction (Fig. 1). Purification and refolding of Rv1399c Given the large quantity of insoluble material obtained [up to 160 mg of p...

Ngày tải lên: 07/03/2014, 16:20

... metalloprotease family and contains a di-zinc binding domain and a small domain that is inserted in the middle of the metal- binding domain and mediates catalysis. The di-zinc Fig. 6. Metal effects ... enzymatic activity. Finally, due to the potential function of carnosine in buffering zinc ions and in enhancing the destructive potential during bacterial infection, the characterizat...

Ngày tải lên: 23/03/2014, 06:20

... demonstrates the results of the purification of the recombinant proteins. The occurrence of dominant bands of the recombinant proteins provides evidence of a high degree of purification obtained by affinity chromatography. ... syn- thesizing activity of the recombinant porcine 2-5A synthetase (A) and N-terminally His-tagged recombinant 2-5A synthetase from G. cydonium (B) during 1...

Ngày tải lên: 23/03/2014, 09:20

... distinguishable from the Soret band of free hemin. Utilizing this difference, the stoichiometry of the heme binding reaction ratio to Syn HO-1 was examined. The inset of Fig. 3 illustrates obtained ... after 210 min, exhibiting the bands of the remaining oxy complex. The 340 nm-band of NADPH decreases in proportion to the decrease of Soret band at 410 nm and to the increases of...

Ngày tải lên: 23/03/2014, 20:22

... protein containing a Mem_trans domain (PF03547.11) was chosen, and after removing the PIN-like proteins, the remaining proteins were considered SbPIN proteins. AUX and LAX1–3 protein sequences in ... Many PIN genes in dicotyledonous Arabidopsis have been studied in detail, including AtPIN1–AtPIN4 and AtPIN7, which act in auxin efflux transport, but the function of AtPIN5, AtPIN 6 and...

Ngày tải lên: 29/03/2014, 09:20

... Expression of soluble ligand- and antibody-binding extracellular domain of human muscle acetylcholine receptor a subunit in yeast Pichia pastoris: role of glycosylation in a-bungarotoxin binding. J ... ligand-binding extracellular domain of the human alpha7 acetylcholine receptor expressed in yeast: replacement of the hydrophobic cysteine loop by the hydrophilic loop of the...

Ngày tải lên: 30/03/2014, 10:20

... Curve fitting revealed an apparent Kd of 740 nM for binding of a single FAD to the protein. The relatively tight binding of FAD and the normal Km for NADPH suggest that the flavoprotein domain of TRX-gp91 phox ... localized within the flavoprotein domain of gp91 phox . In this study, we have investigated the putative flavo- protein domain of gp91 phox . The hydrophobic transmem...

Ngày tải lên: 07/08/2014, 14:22