Báo cáo khoa học: "Topology of Scavenger Receptor Class B Type I (SR-BI) on Brush Border Membrane" docx

... is a functional unit of < /b> SR-BI in rabbit BBMV. Evidence is presented to show that SR-BI in rabbit BBMV is only functional when it is part of < /b> a complex which is linked by disulfide bridges. This ... apo A -I overlay analysis of < /b> rabbit BBMV To confirm SR-BI in rabbit BBMV, we selected three anti SR-BI antibodies which were raised against different domain of < /b> SR-BI (Fi...

Ngày tải lên: 07/08/2014, 15:20

... successively. The immunoreactive bands were visualized using diamino- benzidine (DAB) (Boster Biological Technology, Wuhan, Hubei, China). The following antibodies were used: rabbit SRBI polyclonal antibody ... the expression of < /b> VSMC differentiation marker genes induced by all-trans retinoic acid [4]; KLF4 could induce inhibition of < /b> proliferation of < /b> VSMC, which is mechani...

Ngày tải lên: 18/02/2014, 04:20

... transactivation activity through the repression and association domains (or the titration of < /b> co-repressors), resulting in conformational changes from conformation I (or II) into III or IV. In contrast, ... concentration of < /b> GRIP1 might depend on its homo-oligomerization status, which is mainly deter- mined by the involvement of < /b> its C-terminal region in protein–protein...

Ngày tải lên: 07/03/2014, 12:20

... this effect of < /b> leptin is mediated by activation of < /b> STAT5. An unexpected finding was the capability of < /b> Tyr1138, which is located within a canonical box3 consensus motif, to mediate activation of < /b> ... chemilumines- cence using the primary antibodies mentioned above and horseradish peroxidase-labeled secondary antibody. Blots were re-used after stripping the primary ant...

Ngày tải lên: 07/03/2014, 16:20

... transformation of < /b> Arabidopsis Binary vectors for stable transformation of < /b> Arabidopsis tha- liana were constructed in pCAMBIA2300 vectors (CAM- BIA, Canberra, Australia). To construct the binary vector for ... luciferase activity. The plants containing the CfEcR:SHILmRXR switch exhibited low background activity of < /b> luciferase in the absence of < /b> ligand. On appli- cat...

Ngày tải lên: 15/03/2014, 23:20

... different constraints on the ability of < /b> DNAzymes to hybridize (in comparison with antisense oligonucleotides) because of < /b> the bulky nature of < /b> the activity centre acting as a barrier to accessible sites ... Downregulation of < /b> urokinase plasminogen acti- vator receptor < /b> expression inhibits Erk signalling with concomitant suppression of < /b> invasiveness due...

Ngày tải lên: 16/03/2014, 22:20

... in Fig. 4. Preincubation of < /b> the cells with bikunin during 2 h before 100 n M PMA stimulation results in a concentration-depen- dent inhibition of < /b> the induction of < /b> [ 125 I] DIP-uPA binding (which ... function analysis of < /b> urinary trypsin inhibitor (UTI): identification of < /b> binding domains and signaling property of < /b> UTI by analysis of < /b> truncated prot...

Ngày tải lên: 17/03/2014, 11:20

... (Aurion) at 37 °C. This was followed by incubation with mouse anti-insulin receptor < /b> b- chain mono- clonal antibodies, or anti-IRS1 polyclonal antibodies, or rabbit anti-caveolin polyclonal antibodies ... basal noninsulin-stimulated conditions, colocalized with the insulin receptor < /b> in caveolae. In type 2 diabetes the tissues respond poorly to insulin, exhibiting insulin resist...

Ngày tải lên: 23/03/2014, 12:20

... Transfection experiments with dominant negative forms of < /b> either BRG1 or BRM have resulted in an inhibition of < /b> transcription, lack of < /b> both Pol II loading and chromatin transition, as well as compro- mised ... The observed high level of < /b> DNA methylation within gene bodies and non-coding regions is believed to serve as a suppressor of < /b> transcriptional noise by pr...

Ngày tải lên: 28/03/2014, 22:21

... family member-9 (KIF9). The reliability of < /b> microarray results depends on sev- eral factors such as array production, RNA extraction, probe labeling, hybridization conditions and image analysis [50–53]. ... lL of < /b> supermix (Bio-Rad Laboratories) and 7 lL of < /b> nuclease free water. All the qRT-PCR reactions were performed using the following reaction conditions: Initial incub...

Ngày tải lên: 30/03/2014, 03:20