Báo cáo khoa học: " Expression of Recombinant Porcine Interleukin-2 and Application of Its Antibody to Immunoassays" doc

... data generated were too noisy to allow evaluation of the T m . Charged states of Arg and ArgEE In order to understand the importance of amphiphilicity, titration curves of Arg and ArgEE were compared (Fig. ... pK a of amino groups on Arg and ArgEE were determined as pH 9.2 and 7.4, respectively. The decreased pK a of amino group of ArgEE compare to Arg is related...

Ngày tải lên: 19/02/2014, 16:20

... (1988) Glucocorticoid-modulated gene expression of tissue- and urinary-type plasminogen activator and plasminogen activator inhibitor 1 and 2. J Cell Biol 106, 971–978. 84 Medcalf RL (1992) Cell- and gene-specific ... partitions into secretory vesicles and is present at the cell surface. J Histochem Cytochem 45, 1697–1706. 14 Medcalf RL, Kruithof EK & Schleuning WD (1988) Pla...

Ngày tải lên: 20/02/2014, 02:21

... IL-1b, regulate the expression and the activation of MMPs, a complex cross-regulation does occur between these neuroinflammatory media- tors, and further studies are needed to understand their spatio-temporal ... factors, such as cyclic AMP response element-binding protein, hypoxia inducible factor-1, nuclear factor-E2-like factor 2, c-fos, p53 and peroxisome proliferator-activated...

Ngày tải lên: 07/03/2014, 03:20

... rather low affinity to the enzyme. Effects of other keto acids on hKAT-I activity Ability to function as the amino group acceptor for a number of biologically relevant keto acids and their substrate ... the presence of 2 m M of a-ketobutyrate and 2 m M of a diff erent a- keto acid. *P <0.5, **P < 0.01, significant difference from the control. (B) Effect of different concen...

Ngày tải lên: 07/03/2014, 16:20

... spectra of CCP1CCP2 (#2) col- lected at 300 and 315 K at pH7.0. Fig. S12. Overlay of HSQC spectra collected at 300 K of single CCP1 and CCP2 and of pair of modules at neutral and acidic pH. Doc. ... structural re-arrangement upon autoactivation and to identify possible interaction sites. Results Expression, folding and stability of single and tandem CCP modules...

Ngày tải lên: 15/03/2014, 23:20

... rate; this of course corre- sponds to the rejection conclusion to the right. x 1 and x 2 corre- sponds to unphosphorylated and phosphorylated IR, respectively, and x 3 and x 4 corresponds to internalized ... time-derivative of this vector, x(t) is the state at time t, and f and g are vectors of smooth nonlinear functions. The symbol u denotes the external input to...

Ngày tải lên: 23/03/2014, 06:20

... polysaccharides inhibit the catabolism and loss of both large and small proteoglycans in explant cultures of tendon Tom Samiric, Mirna Z. Ilic and Christopher J. Handley School of Human Biosciences, La Trobe ... decreased expression of aggre- canase activity. Studies have shown that sodium pento- san polysulfate and heparin are internalized by cells and are able to modul...

Ngày tải lên: 23/03/2014, 10:21

... Entamoeba histolytica and Plasmodium falciparum. Although predicted to lie close to one another, the cytosolic location of enolase, with its low redox potential, ensures that the formation of a disulphide bridge ... amplification mixture (50 lL total vol- ume) contained 1 lg of genomic DNA from T. brucei stock 427, 100 pmol of each primer, 250 l M of each of the four deoxyn...

Ngày tải lên: 23/03/2014, 21:20

... Zn 2+ binding to the high-affinity site. These effects of metal ions on S100A2 folding and stability contribute to a better understanding of the Ca 2+ - and Zn 2+ -dependent regulation of the protein. In ... 2 EF-hand 1 EF-hand 2 Scheme 1. S100A2 subunit topology, including the location of cysteines and other Zn 2+ -coordinating residues [8,12]. Boxes repre- sent a-helices a...

Ngày tải lên: 30/03/2014, 02:20

Ngày tải lên: 28/06/2014, 11:20