Báo cáo khoa học: "Expression of Angiostatin Using DNA - Based Semliki Forest Virus Replicon" pptx

... abundace of β-gal 0.5 1.5 1 0 CMV-β-gal ARS-β-gal TOP-β-gal Top-ARS-β-gal 2.5 Relative β-gal mRNA level 1.4 1.2 1 0.8 0.6 0.4 0.2 0 con HS re12h re24 h CMV-β-gal ARS-β-gal TOP-β-gal ... Transcription start site Transcription start site ARS TOP TOP β-gal β-actin CMV-β-gal Transfection ARS-β-gal TOP-β-gal Top- ARS- - gal 1.00 1.00 0.95 1.00 1.02 1.00 1.00 1.21...

Ngày tải lên: 18/02/2014, 12:20

... nigeran (a-1, 3- and a-1,4-glucan), soluble starch (a-1, 4- and a-1,6-glucan), pustulan (b-1,6-glucan), laminarin (b-1,3-glucan), carboxymethilcelullose (b-1,4-glucan) or chi- tin (polymer of NAG ... Aspergillus nidu- lans. The significance of a alpha-1,3-glucan of the cell wall and alpha-1,3-glucanase for cleistothecium develop- ment. Biochim Biophys Acta 273, 174–187. 30 Schoffel...

Ngày tải lên: 19/02/2014, 16:20

... well-conserved C-terminal part (C-domain) that binds the catalytic H-cluster. As seen in other algal [Fe]-hydrogenases, the N-terminal part (F-domain) of HydA2 also lacks the additional [4Fe-4S] ... USA), 25 lL of BAC clone cell-free lysate as template, 125 ng each of forward (5¢-CTGGACGTGACAAACAAGA CCC-3¢, located at the start of the 5¢-UTR) and reverse (5¢-TGACACTGTCTGTGCG-3¢, near...

Ngày tải lên: 20/02/2014, 11:20

... cholinesterase forms of kidney and blood. Mixtures of S1 and S2 extracts of kidney were incu- bated with lectin-free Sepharose 4B (control) and with Sepharose-linked lectins. Samples of Triton X-100-extracted A B Fig. ... position of the prim- ers, as well as the size of the PCR products, are provided in Fig. 5. cDNA was amplified in an Applied Biosys- tems 7500 real-time PCR syste...

Ngày tải lên: 06/03/2014, 22:21

... melanogaster a -F1-ATPase transcript The expression of the a-F1-ATPase gene during develop- ment is coordinated with the expression of the nuclear- encoded b-F1-ATPase gene and the mitochondrial-encoded ATPase ... processing of the a-F1-ATPase mRNA. (A) Northern blots of mRNA from different stages of D. melanogaster embry- ogenesis and in adults using the a-F1-ATPase cDNA as a prob...

Ngày tải lên: 07/03/2014, 16:20

... with glucose, erythromycin, chloramphenicol, cytidine at 1000 lgÆmL )1 and 5-bromo-4-chloro-3-indolyl- b- D -galactoside (X-gal) at 90 lgÆmL )1 . Growth experiments The strains were grown over night ... concentrations of (deoxy)ribonucleo- tides were plotted and fitted to functions using the software program GRAFIF (Erithacus Software Ltd, Harley, Surrey, UK). Wild-type b-galactosidase exp...

Ngày tải lên: 16/03/2014, 16:20

... 91 82 86 01, Tel.: + 33 4 91 82 86 07, E-mail: record@esil.univ-mrs.fr?Abbrevia- tions: ABTS, 2,2-azino-bis-[3-ethylthiazoline-6-sulfonate]; IU, inter- national units; GLA, glucoamylase; MnP, ... many nonphenolic compounds, such as ABTS (2,2-azino-bis- [3-ethylthiazoline-6-sulfonate]) are o xidized by laccases [ 12]. The biological function of most laccases is yet unclear. They have been .....

Ngày tải lên: 17/03/2014, 11:20

... tissue from each trans- genic mouse at the age of 5 months using TRIzol Reagent (In- vitrogen, Carlsbad, CA, USA). First-strand cDNA was synthesized from 2 lg of total RNA using random hexamer primers ... 5¢-TCCCTTCGCACCGTTCTT-3¢ for Col3a1; 5¢-CAAGGTCATCCATGACAACTTTG-3¢ and 5¢-GTCCACCACCCTGTTGCTGTAG-3¢ for GADPH. Transmission electron microscopy Myocardial samples of mice at 5 mont...

Ngày tải lên: 22/03/2014, 16:20

... the effects of i nterferon-c and hypoxia, each of which represses the expression of HO-1 mRNA in other types of human cells. Expression levels of HO-1 mRNA were reduced by interferon- c in two human ... in ARPE-19 cells. The d ifferen- tial effects of TNF-a and IL-1b in the two RPE cell lines may reflect the hetero geneity of R PE cells [31,48]. Effects of hypoxia on the express...

Ngày tải lên: 23/03/2014, 13:20

... 5¢-CTAA GAATTCACATGGATAGGA AA-3¢; PG3B (1984): 5¢-GG GGATCCGCTTCTGCTGC AGTTGTGC-3¢;EPS-1(72):5¢-CC CCATGGCTAAT ATCTTTGATATAAA-3¢;EPS-2(49):5¢-CCACCAG GATTGGGACCACGCC-3¢;SPS-1()33): 5¢-C CCCGGG AGTGAAAAAAGCAAAGTTCAAC-3¢; ... in-frame in the NcoIsiteofthegfp-nos cassette of pKSgfp. pgwsp-gfp-t-nos. Using oligonucleotides EPS-1 and EPS-2, the pgwsp fragment lacking the signal peptide was amp...

Ngày tải lên: 31/03/2014, 07:20