Báo cáo khoa học: "Multidrug-resistant Salmonella typhimurium and Salmonella enteritidis identified by multiplex PCR from animals" doc

Ngày tải lên: 07/08/2014, 15:20

... both BO and BGT were inducible by 3-phenylindole and camalexin. BO was isolated from the wild-type fungus and found to be competitively inhibited by the crucif- erous phytoalexins cyclobrassinin and ... dimer. Identification and chemical modification of the purified enzymes To identify BHLmL2 and BHAb, the bands obtained from SDS ⁄ PAGE were digested with trypsin and then a...

Ngày tải lên: 18/02/2014, 14:20

... Total RNA was extrac- ted and purified from the fresh porcine heart, and genes of four complex II subunits were cloned by RT -PCR against the extracted RNA. (A) Lanes 1 and 2, total RNA extract. (B) ... membrane, and this gradient is harnessed by ATP synthase to make ATP from ADP and P i . Com- plex V is the ATP synthase [9,10]. The structures of mammalian complex III [11,12...

Ngày tải lên: 19/02/2014, 02:20

... was a generous gift from L. W. Ruddock (Biocenter, University of Oulu, Finland). Ni 2+ -chelating Sepharose Fast Flow resin and Q-Sepharose Fast Flow resin were obtained from Amer- sham Biosciences ... full-length cDNA of cPDI was amplified by RT -PCR from total RNAs isolated from the venom ducts of C. marmoreus. The 3¢-end fragment was amplified using a 3¢-RACE adapter primer and...

Ngày tải lên: 07/03/2014, 05:20

... flanked by a 5¢-AseI- and a-3¢ BamHI-site was synthesized by PCR using pCMX- mPPARb [36] as the template. The LexA-DBD fragment, including a Kozak and a nuclear localization sequence, was amplified from ... 6-keto- prostaglandin F 1a ; LBD, ligand-binding domain; mPGES, microsomal prostaglandin E 2 synthase (EC 5.3.99.3); 4-OHT, 4-hydroxytamoxifen; PGE 2 , prostaglandin E 2 ; PGI 2...

Ngày tải lên: 07/03/2014, 12:20

... was supported in part by Grants-in-Aid for Scientific Research (12480169 to T.K. and 13680667 to Y W.Z.) from the Ministry of Education, Science and Culture of Japan, and by the Asahi Glass-, Heiwa–Nakajima-, ... to those obtained by 3¢-RACE. General cDNAs containing 5¢ -and3 ¢-untranslated regions were also amplified by RT -PCR and sequenced [Fig. 1]. These clones were desig...

Ngày tải lên: 07/03/2014, 21:20

... coordinated by the thiols of Cys11 and Cys14 and by the phosphine of TCEP, as recently shown in the case of the homologous protein HAH1 [26]. The presence of a third ligand on the metal ion is also ... (5¢-GGGCCGGCGCGGTTAGATCTAAGCT TAGATAACTTCAGCCTGGTAACCCAGTTCGTT-3¢). Primers 1, 3 and 5 corresponded to the coding strand, respectively, for positions1–54, 76–135, 154–213. Primers 2,...

Ngày tải lên: 23/03/2014, 12:20

... learn to use a system: 1) by unaided trial and error, 2) by having a pre-use tutorial, 3) by trying to use the system and then asking for help when in trouble, or 4) by relying on advice the ... Geneva, Switzerland. Kristiina Jokinen and Graham Wilcock. 2001. Adaptivity and Response Generation in a Spoken Dialogue System. In van Kuppevelt, J. and R. W. Smith (eds.) C...

Ngày tải lên: 23/03/2014, 19:20

... words (such as peace and piece, or quiet and quite) and also the problem of correcting particular word usage errors (such as amount and num- ber, or among and between). Such ... follows from Equations 1 and 2: when comparing P(W) and P(WI), the dominant term corresponds to the most likely tagging; and in this term, if the target word wk and its substitu...

Ngày tải lên: 23/03/2014, 20:21

... or a non-chainlng role when generating top-down from a pivot, and also to control search when generating bottom-up, by ensuring that the left-hand side of any role con- sidered still lies on ... abstractions are indicated by the exclama- tion mark, feature-value disjunction is indicated by the slash, and an equation of the form 'X = Y Z', where X and Y are list...

Ngày tải lên: 24/03/2014, 02:20