... which was indeed predicted. The other main group, obtained 97% of the time in 20 740 explored trees, contained the DIDO PHD sequences clustered with the PHF and the CGBP proteins. As seen in the tree, ... distribution (Fig. 1), in which the SPP1 representatives are at the basal branch of CGBP and DIO. The GCBP proteins contain a PHD domain, fol- lowed by a DNA-...
Ngày tải lên: 07/03/2014, 21:20
... unfolding occurs. The detailed dissociation/unfold- ing mechanism of the protein remains to be determined. In this study, we examined the M r of the protein after equilibration in various denaturing ... further increased. The unfolding curve using the ANS fluorescence probe suggested a multistate process, consistent with the results obtained by protein intrinsic fluoresce...
Ngày tải lên: 08/03/2014, 08:20
Báo cáo khoa học: Cellular response to unfolded proteins in the endoplasmic reticulum of plants pptx
... chain is brefeldin A-insensitive and is inhibited by the proteasome inhibitor clasto-lactacystin b-lactone, resulting in the accumulation of ricin A chains. These stabilized ricin A chains are partly deglycosylated ... (cyclophilin) survey the status of the proline residues and rearrange them from the cis to the trans form to ensure proper folding of the nascent poly...
Ngày tải lên: 16/03/2014, 11:20
Báo cáo khoa học: A di-leucine sorting signal in ZIP1 (SLC39A1) mediates endocytosis of the protein doc
... the surface of these cells were internalized after 1 h of incubation (Fig. 5H), indicating that the endocytic machinery in these mutant ZIP1-Myc expressing cells was intact. Taken together, these ... contrast, in the presence of chloroquine, the total ZIP1-Myc accumula- tion in the CHO cells expressing the wt ZIP1-Myc pro- tein was increased to the level that w...
Ngày tải lên: 16/03/2014, 11:20
Báo cáo khoa học: A novel retinol-binding protein in the retina of the swallowtail butterfly, Papilio xuthus docx
... staining of the gel indicates that the fluorescing protein is one of the major components of soluble proteins in the crude extract. The surface of the fluorescing protein carries negative charge in ... kind of retinol-binding protein possibly localized in the Papilio retina, and functions in the visual cycle and/ or color vision. In this study, we therefo...
Ngày tải lên: 17/03/2014, 03:20
Báo cáo khoa học: "Feasibility Study for Ellipsis Resolution in Dialogues by Machine-Learning Technique" docx
... sufficient, and sometimes more. In this section, we investigate decision trees and discuss them in detail. 5.1 Tree Shape The relation between the number of training samples and the number of nodes ... Japanese thesaurus. 4 Discussion In this section we discuss the feasibility of the el- lipsis resolver via a decision tree in detail from three points of...
Ngày tải lên: 23/03/2014, 19:20
Báo cáo khoa học: Biochemical evidence for conformational changes in the cross-talk between adenylation and peptidyl-carrier protein domains of nonribosomal peptide synthetases ppt
... mutant) and shows the PCP on the acceptor site of the C-domain. The dis- tance of the S1003A residue to the active-site histidine of the C-domain is 16 A ˚ , making it within the reach of the missing ... consisted of the first two functional domains, namely the phenylalanine- specific A domain and the PCP domain. The terminal E domain was excised (the...
Ngày tải lên: 29/03/2014, 08:20
Báo cáo khoa học: Adeno-associated virus gene transfer in Morquio A disease – effect of promoters and sulfatase-modifying factor 1 pot
... clarify the following issues through future long-term in vivo studies: (a) evaluation of silencing of the promoter, and the resultant level of coexpression of SUMF1 and GALNS; and (b) confirmation of ... design the experiments and drafted the manuscript. A. M. Montan ˜ o conceived and designed the experiments, and helped in analysis of the results and...
Ngày tải lên: 29/03/2014, 21:20
Tài liệu Báo cáo khoa học: A genetic screen identifies mutations in the yeastWAR1 gene, linking transcription factor phosphorylation to weak-acid stress adaptation docx
... DNA-binding properties can be different in vitro and in vivo and may involve sequences in the regula- tory domains distinct from the zinc finger or even additional factors [34]. Therefore, the ... sorbic, benzoic and propionic acid [7] and fail to adapt to such stress conditions in the absence of Pdr12p. Moreover, recent data indicate the involvement of Pdr12p in...
Ngày tải lên: 19/02/2014, 00:20
Tài liệu Báo cáo khoa học: "A HYBRID APPROACH TO REPRESENTATION IN THE JANUS NATURAL LANGUAGE PROCESSOR" pot
... defining the set of items that have ever been the actor in a flight. Their definition is solely within NIKL using the QUA link [14], which is exactly the set of fillers of a slot. While having ... using the defini. tJons of those vocabulary items themselves in the dictionary. In this attempt, we encountered the follow- ing difficulties: • Definitions of...
Ngày tải lên: 21/02/2014, 20:20