... characterizationTo examine whether MursiF retains kinase activity in spite of the loss of critical residues involved in ATPbinding, as observed in our in silico analysis of MursiFWKD (Fig. 3A), ... abilityto bind ATP by using a fluorescent ATP analog, 2,4,6-trinitrophenyl ATP (TNP-ATP), which shows anincrease in fluorescence emission intensity accompanied by a blue shift upon protein binding [36]. ... were done usingT-COFFEE. Identical amino acids are indicated by asterisks, high similarity isindicated by double dots, and lower similarity is indicated by single dots. The gaps are introduced...