detection of mycoplasma pneumoniae in respiratory specimens by pcr

... availability of the PCR assay for screening the prevalence of L intracellularis infection in the pig farms Macroscopic Detection of Lawsonia intracellularis in diagnostic specimens by one-step PCR examination ... present in the porcine intestine and feces including swine genomic DNA The number of amplification cycles was one of the important factors for increasing the sensitivity of the PCR assay The increase ... methods include H
E staining and silver staining of the intestinal sections 36 Dong-Kyun Suh et al Fig Section of the ileum from a pig infected with L intracellularis a : Presence of numerous intracellular,...

Ngày tải lên: 07/08/2014, 14:22

... Application of the LAMP assay for detection of GPV infection in goslings Optimal LAMP assay was evaluated by analyzing GPV infected gosling tissues DNA extracted by the tissue Figure Determination of ... survey on GPV infection in goose farming In doing so, the virus-carrying goslings can be identified during the early stages of infection; countermeasures can be devised even before the infection ... a finding confirmed by our results In other words, the specificity and the detection limit of the LAMP assay are equal with FQPCR for detecting GPV The optimal condition for detecting GPV by...

Ngày tải lên: 12/08/2014, 04:21

... the decline in incidence since the 1930 s is changes in the use of pastures Changes in legislation leading to increased use of wood pasturing may reverse the decline Hasle et al Acta Veterinaria ... also lead to an increase in the incidence and distribution of bovine babesiosis As the distributional range of ticks in Scandinavia expands [24], bovine babesiosis may be introduced into areas where ... 1/160, indicating that these are real positives Therefore, by setting a cut-off level of 1:40 (+++) for defining a case of seropositivity for B divergens, antibody testing should result in a specificity...

Ngày tải lên: 12/08/2014, 18:22

... refractive index change at the beginning and end of injections (which occur as a result of a difference in the composition of the running buffer and the injected protein), a control sensorgram obtained ... central pin on the DNA binding surface of RuvA plays a crucial role in junction targeting and processing It constrains the rate of branch migration by RuvAB and in uences resolution by RuvABC ... importance of this structure is reflected in the high conservation of the sequences forming the pin in the majority of bacteria with the exception of two Mycoplasma species and one of Ureaplasma In the...

Ngày tải lên: 17/03/2014, 17:20

... minA llamS J stnemerusaem latef cihpargonosartlu gnisu sehctib ezis llams dna muidem ni ega lanoitatseg fo noitanimreteD A inoirG ,CG inovuL 31 23-72 ,14 ,6002 minA tsemoD dorpeR ycnangerp eninac ... -103 ,8 ,7691 tcarP minA llamS J ygolohprom lacigolotyc dna euqinhceT I ygolotyc lanigav eninaC PA ettuhcS 71 04-53 392 ,33 ,2991 dnuosartlU loidaR teV suretu mutraptsop eninac eht fo yhpargoidar ... serutcurts latef fo noitceted laitini fo emit ehT )82 yaD ,1 giF( oyrbme eht dessapmocne hcihw ,)92-62( 0.1 ± 7.72 yad no detceted saw ,enarbmem cinoinma eht ,enarbmem latef cinegohce ssel ,dnoces A )72-62(...

Ngày tải lên: 07/08/2014, 23:22

... differences in CT values suggesting less virus and lower sensitivity, and no differences in the timing of the collections or in the age or weight of the children In any case, the detection rates of the ... children can be used by parents without reducing influenza virus detection rates The number of influenza-positive nasal swabs and the CT values were similar in the samples collected by the pediatrician ... etiology of a respiratory tract infection is needed, parents can collect respiratory secretions at home using pediatric mid-turbinate nasal flocked swabs This has a number of advantages First of all,...

Ngày tải lên: 12/08/2014, 04:20

... chain chimeric protein, a complicated cellular machinery of normal antigen processing can be bypassed, leading to stable cell surface expression of MHC-I coupled with an antigenic peptide of interest ... suggesting that observed growth inhibition was due to the activation induced cell death (AICD) Thus, we further investigated the apoptotic status of 4O1/C8 by Annexin V staining As shown in Figure ... availability of MHC-I tetramers in rats, development of this system will provide various benefits in analyzing the role of CTLs in a variety of disease models in rats The Tax180-188 epitope used in this...

Ngày tải lên: 13/08/2014, 05:21

... Basing on the analysis of errors done by linguists and researchers, errors are classified into two main types: They are interlingual errors caused by the interference of the mother tongue and intralingual ... caused by the incomplete understanding of language competence, and mistakes are caused by the inappropriate use of language in performance 1.2.3 Error analysis 24 In the process of learning, foreign ... classified into two main types: interlingual and intralingual errors Intralingual errors are traced to four major causes: over-generalization, incomplete application of rules, ignorance of rule...

Ngày tải lên: 18/12/2013, 10:08

... machine learning He represents instances of it as vectors of 35 features These features encode, among other things, information about the parts of speech and lemmata of words in the context of ... was available during training or testing With this setting, the classifier achieved a recall of 55.1%, a precision of 71.9% and a resulting F-measure of 62.4% for the detection of the class nonreferential ... & D Byron (2005) Identifying non-referential it: a machine learning approach incorporating linguistically motivated patterns In Proceedings of the ACL Workshop on Feature Selection for Machine...

Ngày tải lên: 22/02/2014, 02:20

... showing significant higher in vivo concentrations in most strains of P aeruginosa [18] This biomarker could then be used in the detection of P aeruginosa in breath, whether or not in combination ... source of a certain VOC We tried to minimize the effect of saliva contamination by asking the patient to rinse their mouth prior to sputum production Nonetheless, finding a biomarker for P aeruginosa ... Steinheim, Germany) were injected using the same GC-MS method to determine the retention indices of the individual compounds using a modified Kovats method [33] Bacterial culturing Sputa were inoculated...

Ngày tải lên: 05/03/2014, 21:20

... Role of Cough Officer Screening in Early Detection of Pulmonary Tuberculosis in Inpatients Thorac Med 2006, 21:321-327 Page of 13 Granich R, Binkin NJ, Jarvis WR, Simone PM, Rieder HL, Espinal ... might due to the increased awareness of TB in Stage II (Figure 2), or because of interference of TB diagnosis by other severe symptoms among patients in the ICU during Stage I In fact, our COS ... every morning during patient admission, they recorded all patients who complained of cough, including during the pre-admission period If a patient complained of cough at any other time of day,...

Ngày tải lên: 06/03/2014, 04:20

... responses of the sensor materials were measured in a ¯owing stream of pure air or in 40 ppm NO in air (60:40 mixture from an air cylinder with another cylinder (BOC special gases) containing ca ... hump obtained in XRD) Typically, as seen in the ®gure, a region containing ca Ê 6.93 A inter-planar separation can be visualized It is interesting to note that these defective lines in WO3 are ... min) 1.8 7.1 3.0 Table The sensitivity of doped materials Sensitivity (30 min) 2.8 10.0 5.0 Typical conditions: 0.6 g WO3 compressed pellet in a flowing stream of 40 ppm NO in air at 100 ml/min...

Ngày tải lên: 19/03/2014, 16:47

... Duong Lam project, default parameters: search radius: 20m; minimum number of points inside the moving  windows: 5; interpolation method: IDW.  DL1  Default  DL2  Default, other set of errors  DL3  Search radius: 50m  DL4  Min. number of searched points: 10  ... radius  (or  of the  minimum  number  of points  inside  the  search  window),  the  number  of correctly  and  incorrectly  detected  points  is  decreasing.  This  can  be  explained  as  a  ...  inside the moving window; coefficient  (4)  j K V  takes a value in the range from 2 to 4.   j =1 where  mi  is the number of data points that fall  inside  the  moving  window  around  point ...

Ngày tải lên: 22/03/2014, 12:20

... Water in which an interference is not observed at the method detection limit of the compound of interest Prepare reagent water by passing tap water through a filter bed containing about 0.5 kg of ... surrogate in µg/L in the water sample Ax = integrated abundance of the quantitation ion of the analyte in the sample Ais = integrated abundance of the quantitation ion of the internal standard in the ... procedures for determination of organic compounds in finished drinking water, source water, or drinking water in any treatment stage The method is applicable to a wide range of organic compounds...

Ngày tải lên: 22/03/2014, 14:20

... staining was performed: carbol fuchsin 0.3%, slow heating until steaming, rinsing after min, destaining with 25% sulfuric acid, rinsing, counterstaining with methylene blue 0.3% for min, rinsing ... (case finding or treatment monitoring) were included in the study Where serial sputum specimens were tested, each specimen was individually included in the comparative analysis Sample processing ... advantageous in this case, its efficacy for TB case finding in patients with HIV co-infection in Laos remains to be determined CONCLUSION Our study, conducted in a population with a low prevalence of HIV/AIDS,...

Ngày tải lên: 29/03/2014, 03:20

... fMet-tRNA binding to the P-site [10,12,13], probably by stabilizing IF2 binding to the 30S subunit [14], and stimulates the GTPase activity of IF2 [15] It also increases binding of mRNA to the initiation ... kasugamycin-binding site on the 30S subunit or on the 70S ribosome in the region of the mRNA-binding tunnel in the E-site and P-site [32,33] As no effect of kasugamycin on mRNA binding to the ... common denominator for cspA and infA The compensation of the cold sensitivity of IF1 mutant strains by the inactivation of cspA is another functional link to IF1 In view of the finding that IF1...

Ngày tải lên: 29/03/2014, 09:20

... the thinner films still have crystalline structure, as shown in the Raman scattering data in Fig 4(c) The widths of the Raman peaks in the thinner films are slightly wider, indicating longer interatomic ... perturbations are introduced to the molecular system by slightly displacing the atomic positions along the directions indicated in Fig For example, the A1 phonon 1g mode is generated in MD by stretching the ... anharmonicity in thinner films The band gap in 064307-4 ORIGIN OF COHERENT PHONONS IN Bi2 Te3 PHYSICAL REVIEW B 88, 064307 (2013) the very thin Bi2 Te3 films can be wider, for example, ∼0.25 eV in 5-nm-thick...

Ngày tải lên: 06/05/2014, 08:54

... the thinner films still have crystalline structure, as shown in the Raman scattering data in Fig 4(c) The widths of the Raman peaks in the thinner films are slightly wider, indicating longer interatomic ... perturbations are introduced to the molecular system by slightly displacing the atomic positions along the directions indicated in Fig For example, the A1 phonon 1g mode is generated in MD by stretching the ... anharmonicity in thinner films The band gap in 064307-4 ORIGIN OF COHERENT PHONONS IN Bi2 Te3 PHYSICAL REVIEW B 88, 064307 (2013) the very thin Bi2 Te3 films can be wider, for example, ∼0.25 eV in 5-nm-thick...

Ngày tải lên: 06/05/2014, 08:58

... inhibition of insulin receptor substrate-1 expression leads to insulin resistence, hyperinsulinemia, and increased adiposity Endocrinology 2005, 146:1428-1437 Hsu SM, Raine L, Fanger H: Use of Avidin-Biotin-Peroxidase ... activity index Mayo Clin Proc 1994, 69:409-415 Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal ... at the State University of Campinas, Coloproctology Unit, and at the Cell Signaling Laboratory of the Department of Internal Medicine Page of (model PT 10/35; Brinkmann Instruments, Westbury,...

Ngày tải lên: 18/06/2014, 16:20

... weighted moving average (WMA) By calculating the ratio of the AC components and the ratio of the DC components of the two light sources, SpO2 can be obtained from every single pulse of a PPG signal ... samples in every several-second intervals In this study, the SpO2 obtained by the WMA approach was the average of SpO2 samples in an 8-second period Overlap processing was performed at 1-second interval ... frequency band is usually predetermined by certain thresholds or obtained from an independent pulse rate estimator, e.g by applying electrocardiography in parallel In this study, FFT is applied to...

Ngày tải lên: 19/06/2014, 10:20