Báo cáo khoa học: "Expression and characterization of the flavoprotein domain of gp91phox" potx

... experimentally. Indeed, the presence of Arg213 along with the proximity of the charge relay Asp260 within the active site could be responsible for the modification of the catalytic properties of the catalytic histidine. ... avor the displacement of the equilibrium toward the neutral state of His290. To attest this hypothesis, an Arg213Ala mutant has been express...

Ngày tải lên: 07/03/2014, 16:20

... activity [33]. These results indicate the importance of these residues in the stability of di-zinc binding and suggest that these residues are essential for the enzymatic activity of the PepD. To ... content of the purified PepD wild- type and mutant proteins. Loss of enzymatic activity in the mutants was not due to changes in the second- ary structure of the pr...

Ngày tải lên: 23/03/2014, 06:20

... dsRNA may meet these requirements only partially. Further studies will be required to clarify the structure of the activator of 2-5A synthetases in the sponges as well as the nature of the RNA binding site ... demonstrates the results of the purification of the recombinant proteins. The occurrence of dominant bands of the recombinant proteins provides evidence...

Ngày tải lên: 23/03/2014, 09:20

... maximum of the Soret band with a small absorption coefficient (Table 1). On the other hand, relative intensities of the 498 and 630 nm bands compared with those of the 575 and 535 nm bands in the ... exhibiting the bands of the remaining oxy complex. The 340 nm-band of NADPH decreases in proportion to the decrease of Soret band at 410 nm and to the increas...

Ngày tải lên: 23/03/2014, 20:22

... membrane-span- ning domain, and an intracellular domain [3]. The N-terminal ECD of each of the two a subunits con- tains the major part of the binding site for the cho- linergic ligands. The two sites ... at the interface between one a subunit and the c ⁄ e subunits and the other between the second a subunit and the d subunit [4]. The c ⁄ e and d sub...

Ngày tải lên: 30/03/2014, 10:20

... activation domain eliminated the ability of p67 phox to activate the NBT- reductase activity of the flavoprotein domain. These data indicate that the target of the activation domain of p67 phox resides ... (filled bars) or absence (open bars) of Rac1 (5.4 mM). Error bars show the standard error of the mean (n = 3). Expression and characterization of t...

Ngày tải lên: 07/08/2014, 14:22

... together with the low unfolding enthalpy of the domain, is consistent with its lower degree of structure [5]. Under the same equilibrium conditions, the values of T m and DH m for either of the ... only the DSC data corresponding to the first of the two transitions present in Fig. 5. The effect of the scan rate of the DSC calorimeter on the heat capacity...

Ngày tải lên: 21/02/2014, 03:20

... of the P2Y 12 receptor and low expression of the P2Y 1 recep- tor. In cells grown in medium containing 10% (v ⁄ v) fetal bovine serum, the expression ratio of the two receptors is reversed. The ... part of the self-sufficiency mechanism of glioma C6 cells, enabling survival in unfavorable metabolic condition and sta- ving off cell death. In conclusion, these data reve...

Ngày tải lên: 30/03/2014, 08:20

... the phosphorylation and ubiquitin-related degradation of IjB. The active form of NF-jBp65 is then released and translocated into the nucleus to activate the tran- scription of IL-1b and TNF-a. In this ... and secretion of IL-10 Because of the lack of secretion of the inflammatory cytokines IL-1b and TNF-a from hypoxia ⁄ SD-stimu- lated MSCs, as well as the...

Ngày tải lên: 18/02/2014, 04:20

... comprising the two sugar binding pockets in the pulchellins (Fig. 3). In order to compare the similarity of the A- and B-chains of the four isoforms, a pairwise alignment was performed and the values of ... Taken together with the inability of P III and P IV to inhibit hemagglutination in vitro in the presence of this sugar, these data suggest that the bind-...

Ngày tải lên: 18/02/2014, 16:20