Báo cáo khoa học: "Expression and characterization of the fl

Báo cáo khoa học: Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis A novel carboxyl esterase structurally related to the HSL family docx

... experimentally. Indeed, the presence of Arg213 along with the proximity of the charge relay Asp260 within the active site could be responsible for the modiﬁcation of the catalytic properties of the catalytic histidine. ... avor the displacement of the equilibrium toward the neutral state of His290. To attest this hypothesis, an Arg213Ala mutant has been express...

Ngày tải lên : 07/03/2014, 16:20

9
584
0

Báo cáo khoa học: Expression and characterization of the bioﬁlm-related and carnosine-hydrolyzing aminoacylhistidine dipeptidase from Vibrio alginolyticus pot

... activity [33]. These results indicate the importance of these residues in the stability of di-zinc binding and suggest that these residues are essential for the enzymatic activity of the PepD. To ... content of the puriﬁed PepD wild- type and mutant proteins. Loss of enzymatic activity in the mutants was not due to changes in the second- ary structure of the pr...

Ngày tải lên : 23/03/2014, 06:20

14
304
0

Báo cáo khoa học: Expression and characterization of recombinant 2¢,5¢-oligoadenylate synthetase from the marine sponge Geodia cydonium ppt

... dsRNA may meet these requirements only partially. Further studies will be required to clarify the structure of the activator of 2-5A synthetases in the sponges as well as the nature of the RNA binding site ... demonstrates the results of the puriﬁcation of the recombinant proteins. The occurrence of dominant bands of the recombinant proteins provides evidence...

Ngày tải lên : 23/03/2014, 09:20

13
429
0

Báo cáo khoa học: Expression and characterization of cyanobacterium heme oxygenase, a key enzyme in the phycobilin synthesis Properties of the heme complex of recombinant active enzyme potx

... maximum of the Soret band with a small absorption coefﬁcient (Table 1). On the other hand, relative intensities of the 498 and 630 nm bands compared with those of the 575 and 535 nm bands in the ... exhibiting the bands of the remaining oxy complex. The 340 nm-band of NADPH decreases in proportion to the decrease of Soret band at 410 nm and to the increas...

Ngày tải lên : 23/03/2014, 20:22

12
459
0

Báo cáo khoa học: Expression and characterization of soluble forms of the extracellular domains of the b, c and e subunits of the human muscle acetylcholine receptor pot

... membrane-span- ning domain, and an intracellular domain [3]. The N-terminal ECD of each of the two a subunits con- tains the major part of the binding site for the cho- linergic ligands. The two sites ... at the interface between one a subunit and the c ⁄ e subunits and the other between the second a subunit and the d subunit [4]. The c ⁄ e and d sub...

Ngày tải lên : 30/03/2014, 10:20

12
394
0

Báo cáo khoa học: "Expression and characterization of the flavoprotein domain of gp91phox" potx

... activation domain eliminated the ability of p67 phox to activate the NBT- reductase activity of the flavoprotein domain. These data indicate that the target of the activation domain of p67 phox resides ... (filled bars) or absence (open bars) of Rac1 (5.4 mM). Error bars show the standard error of the mean (n = 3). Expression and characterization of t...

Ngày tải lên : 07/08/2014, 14:22

8
216
0

Tài liệu Báo cáo khoa học: Unfolding and aggregation during the thermal denaturation of streptokinase pptx

... together with the low unfolding enthalpy of the domain, is consistent with its lower degree of structure [5]. Under the same equilibrium conditions, the values of T m and DH m for either of the ... only the DSC data corresponding to the ﬁrst of the two transitions present in Fig. 5. The eﬀect of the scan rate of the DSC calorimeter on the heat capacity...

Ngày tải lên : 21/02/2014, 03:20

13
503
0

Báo cáo khoa học: Expression and functional characterization of P2Y1 and P2Y12 nucleotide receptors in long-term serum-deprived glioma C6 cells ppt

... of the P2Y 12 receptor and low expression of the P2Y 1 receptor. In cells grown in medium containing 10% (v ⁄ v) fetal bovine serum, the expression ratio of the two receptors is reversed. The ... part of the self-sufﬁciency mechanism of glioma C6 cells, enabling survival in unfavorable metabolic condition and sta- ving off cell death. In conclusion, these data reve...

Ngày tải lên : 30/03/2014, 08:20

13
378
0

Tài liệu Báo cáo khoa học: Expression and secretion of interleukin-1b, tumour necrosis factor-a and interleukin-10 by hypoxia- and serum-deprivation-stimulated mesenchymal stem cells Implications for their paracrine roles ppt

... the phosphorylation and ubiquitin-related degradation of IjB. The active form of NF-jBp65 is then released and translocated into the nucleus to activate the tran- scription of IL-1b and TNF-a. In this ... and secretion of IL-10 Because of the lack of secretion of the inﬂammatory cytokines IL-1b and TNF-a from hypoxia ⁄ SD-stimulated MSCs, as well as the...

Ngày tải lên : 18/02/2014, 04:20

11
653
0

Tài liệu Báo cáo khoa học: Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds docx

... comprising the two sugar binding pockets in the pulchellins (Fig. 3). In order to compare the similarity of the A- and B-chains of the four isoforms, a pairwise alignment was performed and the values of ... Taken together with the inability of P III and P IV to inhibit hemagglutination in vitro in the presence of this sugar, these data suggest that the bind-...

Ngày tải lên : 18/02/2014, 16:20

12
763
0