Báo cáo khoa học: Correspondence between two antimatroid algorithmic characterizations ppt

... interesting to deter- mine whether a hydrophobic interaction between the Leu residues from v-KIND and MAP2 contributes to the interaction between the two proteins. In conclusion, the present study has ... Quinlan ME, Hilgert S, Bedrossian A, Mullins RD & Kerkhoff E (2007) Regulatory interactions between two actin nucleators, Spire and Cappuccino. J Cell Biol 179, 117–128. 11 Ley...

Ngày tải lên: 14/02/2014, 19:20

... hydrophobic agents depends on two competitions: (a) competition between the active pumping of the agents out of the cells and their pas- sive uptake [10,31] and (b) competition between the active pumping ... taken up into the cells, the obstacle to uptake of rhodamines into the cells can be due to one of two possibilities: (a) NBD-Cl-sensitive export is not mediated by either of these...

Ngày tải lên: 18/02/2014, 13:20

... ractions obtained (A–C)were characterized by N-terminal sequencing a nd two parameters of enzyme function distinguishing sensitively between Cc StP and EcMalP: (a) apparen t substrate affinity and su ... buffer, pH 7.0, contain- ing a concentration o f potassium phosphate between < 0.05 and 80 m M . PLP at a concentration of between 0.0 and 100 l M was added to reconstitute the holo...

Ngày tải lên: 19/02/2014, 16:20

... interactions between proteins are involved in nearly all biological functions, the physico- chemical principles governing the interaction of proteins are not fully understood. In the literature, two types ... bi-enzyme complex (460 kDa) which i s made up of two molecules of tetrameric glyceraldehyde-3-phos- phate dehydrogenase (GAPDH) (EC 1.2.1.13), two mole- cules of dimeric phosphor...

Ngày tải lên: 19/02/2014, 16:20

... 1081–1088. 33. Fransson, L A ˚ ., Nieduszynski, I.A., Phelps, C.F. & Sheehan, J.K. (1979) Interactions between dermatan sulfate chains. III. light scattering and viscometry studies of self association. ... ability of these bacteria to bind to DS, a molecule that is abundant throughout the skin. Fifty -two M protein-expressing strains, representing 49 different serotypes, as well as eig...

Ngày tải lên: 20/02/2014, 11:20

... analysis A region of low amino acid sequence homology between HydA1 and HydA2 (the insert between motifs 2 and 3) was screened for high antigenicity using two methods, Alpha Diagnostic (San Antonio, Texas) ... sulfur deprivation. However, the expression of the two transcripts differs during the sulfur-deprivation period. This suggests differences in the regulation of expression of the...

Ngày tải lên: 20/02/2014, 11:20

... determined the relationship between changes in protein stability (DDG D °) and overall catalytic efficiency (Dlog(k cat ⁄ K m )) in the presence of Fig. 2. Relationship between protein stability ... investigated on a number of enzymes. Conse- quently, this has led to the classification of osmolytes into two classes: compatible or counteracting. Compat- ible osmolytes increase protein stab...

Ngày tải lên: 07/03/2014, 00:20

... AdoCbl between these two enzymes appear to exit. Methylmal- onyl CoA mutase binds AdoCbl very tightly with a K d of 0.17 lm, while glutamate mutase binds AdoCbl relatively weakly with a K d between ... for AdoCbl is 18 lm under similar conditions [12]. However, the only difference between AdoCbl and (Bza)AdoCba is two methyl groups. In contrast, (Co-b-5¢-Deoxyadenosin-5¢-yl)-(p-cres...

Ngày tải lên: 07/03/2014, 04:20

... of the Ser16 and Ser127 residues, we generated two single-mutant pro- teins where the two serine residues were replaced with alanine, producing two single-mutant proteins (Ser16- Ala and Ser127Ala) ... (wat 2) is bound between a third water molecule (wat 3) and a C1 carboxyl oxygen of the substrate that is also hydrogen bonded to His106. The third water molecule bridges the first two...

Ngày tải lên: 07/03/2014, 05:20

... Two- hybrid assay To confirm the direct interaction between proteins or pro- tein fragments, the appropriate bait and prey constructs were co-transformed into yeast cells, and two- hybrid ... confirm the specificity of interaction between CcCac1L and CcLim15, we performed yeast two- hybrid and b-galactosidase assays (Fig. 1C,D). Next, S. Ishii et al. Link between Lim15/Dmc1 and the CAF...

Ngày tải lên: 07/03/2014, 05:20