Báo cáo lâm nghiệp: " Flight activity of Anthribus nebulosus Forster, 1770 (Coleoptera: Anthribidae) and notes to its life history" potx

Báo cáo Y học: Amidolytic activity of prostatic acid phosphatase on human semenogelins and semenogelin-derived synthetic substrates docx

Báo cáo Y học: Amidolytic activity of prostatic acid phosphatase on human semenogelins and semenogelin-derived synthetic substrates docx

... authors are indebted to C. Barthe  le  myandM.H.Saussereau (Department of Human Reproduction CHRU Bretonneau, Tours) for their collaboration and helpful advice, and in Brazil to the FundacË a Ä ode Amparo ... Moreau 1 and Francis Gauthier 1 1 Laboratory of Enzymology and Protein Chemistry, INSERM EMI-U 00-10, University FrancË ois Rabelais, Faculty of Medicine, Tours, Fran...
Ngày tải lên : 24/03/2014, 00:21
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Báo cáo khoa học: Structural framework of the GABARAP–calreticulin interface – implications for substrate binding to endoplasmic reticulum chaperones potx

Báo cáo khoa học: Structural framework of the GABARAP–calreticulin interface – implications for substrate binding to endoplasmic reticulum chaperones potx

... portion of calreti- culin forms a protrusion emerging from the base of the hp1 hp2 Fig. 4. Comparison of GABARAP structures. Top: overview of nonliganded and liganded GABARAP structures in ribbon and ... presence of specific receptors. On the basis of our results, we speculate that GABARAP (or another member of its family) may constitute such a receptor, tethering calreticu...
Ngày tải lên : 30/03/2014, 02:20
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Báo cáo y học: "Anticancer Activity of the PR Domain of Tumor Suppressor RIZ1"

Báo cáo y học: "Anticancer Activity of the PR Domain of Tumor Suppressor RIZ1"

... inhibitor specific for the PR domain may help to understand the exact mechanism for its anti- cancer function. Fig. 3. Anticancer activity of the PR domain against human hepatocarcinoma ... different types of cancer, and evaluated the anticancer activity of the PR domain against human hepatoma HuH7 cells through both direct administration of recombi- nant His 6 -...
Ngày tải lên : 25/10/2012, 11:15
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Tài liệu Báo cáo khoa học: Autolytic activity of human calpain 7 is enhanced by ESCRT-III-related protein IST1 through MIT–MIM interaction pptx

Tài liệu Báo cáo khoa học: Autolytic activity of human calpain 7 is enhanced by ESCRT-III-related protein IST1 through MIT–MIM interaction pptx

... volume of a vehicle used for dissolving reagents was added to the reaction mixture in place of inhibitors. Bands of full- length mGFP–calpain 7–Strep and those of 30 K are shown in the upper and ... and WB with mAb against GFP to detect autolyzed mGFP–cal- pain 7–Strep. Acknowledgements We thank E. Goto for technical assistance. We also thank K. Hitomi and all members of...
Ngày tải lên : 18/02/2014, 04:20
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Tài liệu Báo cáo khoa học: ATPase activity of magnesium chelatase subunit I is required to maintain subunit D in vivo ppt

Tài liệu Báo cáo khoa học: ATPase activity of magnesium chelatase subunit I is required to maintain subunit D in vivo ppt

... Mg-chelatase of tobacco: identification of a Chl D cDNA sequence encoding a third subunit, analysis of the interaction of the three subunits with the yeast two-hybrid system, and recon- stitution of the ... D207N, R289K and L111F, and analysed their ability to interact with BchD. The ATPase-deficient BchI proteins provide a tool to dissect the interaction between BchI and...
Ngày tải lên : 19/02/2014, 12:20
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Tài liệu Báo cáo khoa học: High activity of human butyrylcholinesterase at low pH in the presence of excess butyrylthiocholine pptx

Tài liệu Báo cáo khoa học: High activity of human butyrylcholinesterase at low pH in the presence of excess butyrylthiocholine pptx

... concerted proton transfer to the leaving group of the substrate occurs. Proton transfer The requirement of an acceptor for the Oc proton from the catalytic serine is a critical component of the mechanism ... the mechanism of action of chymotrypsin: the role of the low barrier hydrogen bond. Biochemistry 36, 4576–4584. 46. Warshel, A. (1998) Electrostatic origin of the catalyti...
Ngày tải lên : 20/02/2014, 23:20
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Báo cáo khóa học: Chaperone activity of cytosolic small heat shock proteins from wheat pptx

Báo cáo khóa học: Chaperone activity of cytosolic small heat shock proteins from wheat pptx

... Data points and error bars reflect the mean and standard deviation of three replicates. Results Comparison of TaHsp16.9 C-I and TaHsp17.8 C-II To produce recombinant wheat class I and II sHSPs ... the ratio of sHsp to substrate that is required to suppress light scattering can be used as a measure of effectiveness of the chaperone. Using this assay we tested the relativ...
Ngày tải lên : 07/03/2014, 15:20
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Báo cáo khoa học: Antimicrobial activity of histones from hemocytes of the Pacific white shrimp ppt

Báo cáo khoa học: Antimicrobial activity of histones from hemocytes of the Pacific white shrimp ppt

... search of the obtained sequences. The lower band yielded peptides similar to histone H4, the upper b ands contained peptides from histones H2A and H2B, and the highest molecular mass band produced ... than 11 kDa and contains peptides similar to the central domain o f t he Drosophila histone H1 protein. It is necessary to purify this peptide to homogeneity to confirm its a...
Ngày tải lên : 07/03/2014, 16:20
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Báo cáo khoa học: Cytotoxic activity of nucleoside diphosphate kinase secreted from Mycobacterium tuberculosis pptx

Báo cáo khoa học: Cytotoxic activity of nucleoside diphosphate kinase secreted from Mycobacterium tuberculosis pptx

... completion of the binding reaction, each filter was washed several times with an excess of TMD buffer, air dried and autoradiographed. Cytotoxicity assay Cytotoxic activity of purified Ndk and concentrated ... presence of a mixture of 3 m M ADP and 1 m M each of G-, C- and UTP. It was observed that Ndk was cytotoxic to the macrophages in the presence of the mixture, wh...
Ngày tải lên : 08/03/2014, 08:20
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Báo cáo khoa học: ADPase activity of recombinantly expressed thermotolerant ATPases may be caused by ppt

Báo cáo khoa học: ADPase activity of recombinantly expressed thermotolerant ATPases may be caused by ppt

... [25]. To track the activity of AK during its enrich- ment (and prior to its identification), the fractions were diluted and mixed with the S-fraction of NtrC1 Cshort-his6 (ADPase-free) to measure ... [14]). Given its high catalytic activity and K m for ADP of 90 lm [15], nanomolar concentrations of AK are sufficient to generate ATP from ADP to fuel the NtrC1 C A...
Ngày tải lên : 16/03/2014, 04:20
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