Báo cáo sinh học: "Functional genomics of cell morphology using RNA interference: pick your style, broad or deep" doc

... revealed phenotypes such as changes in cell shape or the accumulation of multinucleated cells upon depletion of only 160 of the RNA targets (one third of which correspond to a gene lacking a previously ... lack of function. The occurrence of proteins lacking RNAi phenotypes opens the door to combinatorial RNAi screens for synthetic interac- tions and the construction of pr...

Ngày tải lên: 06/08/2014, 18:20

... generation of tumor-associated myeloid suppressor cells. Induction: Normal donor PBMC are co-cultured with human solid tumor cell lines for one week. MDSC Isolation: CD33 + or CD11b + cells are isolated ... 8 of 20 Figure 6 Morphology, phenotype, and function of CD11b + and CD33 + MDSC subsets. A, Morphology of human CD33 + and CD11b + MDSC subsets isolated after tumor cell l...

Ngày tải lên: 18/06/2014, 19:20

... using a co-RNAi screen to identify dsRNA suppressors of a cell shape change induced by Pten dsRNA. Conclusions: Using RNAi, we identified genes that influence cytoskeletal organization and morphology ... characteristic shape of two morphologically distinct cell lines, we performed RNAi screens in each line with a set of double-stranded RNAs (dsRNAs) targeting 994 predicted ce...

Ngày tải lên: 06/08/2014, 18:20

... receptor, of the human b 2 -adrenergic receptor and of the rat neurotensin receptor have been produced in CF systems in yields approaching 1 mg of protein [16]. As observed for production of the porcine ... Characterization of human endothelin B receptor and mutant receptors expressed in insect cells. Eur J Biochem 248, 139–148. Ligand binding of cell- free endothelin B receptor...

Ngày tải lên: 16/03/2014, 10:20

... http://www.virologyj.com/content/4/1/97 Page 11 of 17 (page number not for citation purposes) Alignment of 0305φ8-36 orf99 to diagnostic motifs of the RNA- dependent RNA polymerase family Pfam05183Figure 5 Alignment of 0305φ8-36 orf99 ... 0305 φ 8-36 gene organization suggests an origin from two major ancestors The gene organization of phage 0305φ8-36 [1,2] is shown in Figure 1....

Ngày tải lên: 18/06/2014, 18:20

... regulatory genes. One of the major regulatory genes of HIV-1 is tat, which encodes the viral transactivator of transcription known as Tat [3,4]. Originally discovered as an essential cofactor for ... clone A1-1 for donor A, clone B2-1 for recipient B and clones C1-2 and C2-4 for recipient C. These clones were present in most of the time points (or all of the time points for donor A...

Ngày tải lên: 18/06/2014, 18:20

... monolayers of HEL cells were mock infected or infected with 6 PFU /cell of KM110-R to establish quiescence. Four days later the cells were mock infected or superinfected with 30 PFU /cell of n212 or ... autofluorerescent signals emitted by individual HEL cells are highly correlated (see for example figure 1) such that cells expressing neither dsRED2 nor eGFP fall on the diago...

Ngày tải lên: 19/06/2014, 08:20

... confusion arises from wide- spread use of the term 'G2/M', which originally came into Figure 2 Unattached kinetochores occur in the course of correction of incorrectly attached chromosomes. ... distinguish normal from erroneous kinetochore attachments, correction of the latter can result in the production of an unattached kinetochore detected by the checkpoint. See tex...

Ngày tải lên: 06/08/2014, 19:21

... immersed, for 12 h, in methanol (or water) solution of rhodamine (R6G), with molarities of the order of magnitude of 10 − 4 M, and then dried by free evaporation of the solvent, for a few minutes. ... nitrogen-cooled CCD detector was used, equipped with motorized stepping drive motors for the scanning of the grating-like structures. For this series of measurements, an exci...

Ngày tải lên: 18/06/2014, 22:20

... vitellogenin-stimulatory factor of shrimp. Because of the existence of multiple forms of the CHH family neuropeptides, there may be a dis- crepancy in defining the function of many cDNAs cloned using a molecular ... expression lev- els of the gene or protein. The expression level was normal- ized with either the rRNA (for the northern blot) or hemocyanin (for the western blot...

Ngày tải lên: 16/03/2014, 05:20