... FEBSPurification, microsequencing and cloning of spinachATP-dependent phosphofructokinase link sequence and function for the plant enzymeChristian Winkler, Britta Delvos, William Martin and Katrin HenzeInstitute ... sequence, subunit composition, and putative chloroplast localization, and comparison withPFK sequences from other sources.Results and DiscussionPurification and microsequencing of spinach PFKThe ... against the peptides LSGNAVLGDIGVHFK and EIYFEPTK, and produced a PCR fragment of750 bp that was cloned and sequenced. The codingsequence was completed by 5¢- and 3¢-RACE, yieldingSoPFK1, which...