Atomic Force Microscopy in Cell Biology Episode 2 Part 12 ppt

... studies, 20 3 20 4 R Recording mode photonic force microscopy contact mode, 20 8 20 9 fast 3D single-particle tracking, 21 0 tapping mode, 20 9 21 0 Resolution, PFM studies, 20 7 20 8 RL cells, adhesion force ... Rieder 4 12 Volumes in Series Volume 29 (1989) Fluorescence Microscopy of Living Cells in Culture, Part A: Fluorescent Analogs, Labeling Cells, and Basic Micro...

Ngày tải lên: 06/08/2014, 02:20

... crystal. 13. Atomic Force Microscopy and Spectroscopy of Membrane Proteins 27 9 Fig. 14 Native bacteriorhodopsin assembled into an orthorhombic lattice. (A) In this crystal form (p 22 1 2 1 ) the ... native 13. Atomic Force Microscopy and Spectroscopy of Membrane Proteins 27 1 Fig. 8 Contact mode AFM topograph of α-hemolysin oligomers. The α-hemolysin inserted into the phos...

Ngày tải lên: 06/08/2014, 02:20

... (20 00). New insight into cellulose structure by atomic- force microscopy shows the I-alpha crystal phase at near -atomic resolution. Biophys. J. 79, 1139– 1145. Betzig, E., Finn, P. L., and Weiner, ... immunoglobulin and fibronectin III domains in the muscle protein titin measured by atomic force microscopy. Biophys. J. 75, 3008–3014. Rief, M., Pascual, J., Saraste, M., and Gaub,...

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... 119– 120 probe surfaces, 120 – 121 applications, 116 dynamic force microscopy bond lifetime, 124 kinetic rates, energies, binding pocket, 125 unbinding force vs. loading rate, 124 – 125 unbinding force distribution, ... endothelial cadherin binding strength, 128 – 129 Ca 2+ dependence, 127 – 128 conformation, 126 overview, 125 – 126 thermodynamics and structur...

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... The AFM Imaging of Living Cells 21 5 2. Henderson, E., Haydon, P. G., and Sakaguchi, D. S. (19 92) Actin filament dy- namics in living glial cells imaged by atomic force microscopy. Science 25 7, 944– 1946. 3. ... Methods in Molecular Biology, vol. 24 2: Atomic Force Microscopy: Biomedical Methods and Applications Edited by: P. C. Braga and D. Ricci © Humana Press Inc., To...

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... with atomic force microscopy operating in different modes. Ultramicroscopy 68, 121 – 128 . 15. Vater, W., Fritzsche, W., Schaper, A., Bohm, K. J., Unger, E., and Jovin, T. M. (1995) Scanning force microscopy ... decorated microtubulesusing tapping mode atomic force microscopy in fluids. Eur. Biophys. J. 28 , 611– 620 . 12. Lyubchenko, Yu., L., Shlyakhtenko, L. S., Har...

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... sectioning using quantitative differential interference contrast light microscopy (20 ,21 ). Here, we present an alternative new method using atomic force microscopy (AFM) for measuring cell volume in ... is commonly used for blood cells in suspension. In contrast with spherical cells in suspension, ECs under From: Methods in Molecular Biology, vol. 24 2: Atomic Forc...

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... 1 32, 24 3 25 2. 20 . Marsh, D. J., Jensen, P. K., and Spring, K. R. (1985) Computer-based determina- tion of size and shape in living cells. J. Microsc. 137, 28 1 29 2. 21 . Timbs, M. M. and Spring, ... cells in phosphate-buffered saline (PBS) 1 mM CaCl 2 , 3 mM KCl, 1 mM K 2 HPO 4 ; 2 mM MgCl 2 , 140 mM NaCl, 8 mM Na 2 HPO 4 ; pH 7.4 (2 × 5 min). 2. Depending upon the ep...

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... Ultramicroscopy 82, 28 9 29 5. Lehenkari, P. P., and Horton, M. A. (1999). Single integrin molecule adhesion forces in intact cells measured by atomic force microscopy. Biochem. Biophys. Res. Commun. 25 9, ... and Yu, L. (20 00). Atomic force microscopy imaging of living cells: A preliminary study of the disruptive effect of the cantilever tip on cell morphology. Ultramicrosc...

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... aldehyde groups. Cells for live imaging are washed again in PBS containing 10 mg/ml FSG. The scanning experiments are carried out in culture medium for living cells and in PBS for fixed cells. In both ... Plate.) Fig. 3 AFM imaging of laminin molecules in air shows submolecular structure in the laminin arms (top row). In the sequential images, a single laminin molecule in aqueo...

Ngày tải lên: 06/08/2014, 02:20