Atomic Force Microscopy in Cell Biology Episode 2 Part 2 pot
Atomic Force Microscopy in Cell Biology Episode 1 Part 2 pptx
... Surface Sci 460,
29 2–300.
21 . Pang, G. K., Baba-Kishi, K. Z., and Patel, A. (20 00) Topographic and phase-
contrast imaging in atomic force microscopy. Ultramicroscopy 81 (2) , 35–40.
22 . Butt, H-J. ... by pulsed-
force- mode atomic force microscopy. Ultramicroscopy 82, 20 3 21 2.
36 Ricci and Braga
Low-pass filters, although capable of reducing noise in the data, will i...
Atomic Force Microscopy in Cell Biology Episode 1 Part 2 pps
... wide interest in biology because of their fun-
damental functions in the cell. They are responsible, e.g., for both targeting organelles
through the cells and setting up the mitotic spindle during ... structure that interacts with its light chain, binding to the cargo.
Several studies on kinesins have shown that the neck and the first hinge region of the
motor play important roles in...
Atomic Force Microscopy in Cell Biology Episode 1 Part 4 potx
... filament
dynamics in living glial cells imaged by atomic force microscopy. Science 25 7,
1944–1946.
3. Hoh, J. H. and Hansma, P. K. (19 92) Atomic force microscopy for high-resolu-
tion imaging in cell biology. ... carcinoma cells by scanning force microscopy. J. Cell Sci.
107, 24 27 24 37.
7. Gibson, C. T., Watson, G. S., and Myhra, S. (1996) Determination of the
spr...
Atomic Force Microscopy in Cell Biology Episode 1 Part 7 pot
... dentin (9–13).
1 .2. Continuous and Sequential Longitudinal Studies and Reference
Layers
Continuous scanning is conducted by obtaining images continuously during dem-
ineralization in the wet cell ... 42( Suppl. 2) , 4 82 483.
16. Bustamante, C. and Keller D (1995) Scanning force microscopy in biology. Phys-
ics Today 32 38.
17. Benoit, M., Holstein, T., and Gaub, H. E. (1997) Lat...
Atomic Force Microscopy in Cell Biology Episode 1 Part 10 potx
... (7).
3.5 .2. Loading Forces
Using different loading forces on the same sample will result in images with
different information (Fig. 3). As outlined in Fig. 3, applying low imaging
forces will result in ... cat. no. 524 00- 025 ).
2. 2. Growth Medium and Reagents
1. For slow-growing or nondividing cells (7,9): RPMI-1640 with 25 mM HEPES
(see Note 2) , 2 mM L-glutamine (Gibco BRL, c...
Atomic Force Microscopy in Cell Biology Episode 1 Part 6 potx
... longer.
3
If the binding pocket is potentially inactivated by binding the amylose with NH
2
groups too close to the
pocket, a soluble binding partner lacking any NH
2
groups could be added during activation ... platform to investigate cell- to -cell
interactions in vivo down to the molecular level will be described, immobilizing living
cells to a force sensor. Epithelial cells (RL95...
Atomic Force Microscopy in Cell Biology Episode 1 Part 1 ppt
... Rev. Sci. Instr. 70, 121 – 124 .
13. Lehenkari, P. P., Charras, G. T., Nykanen, A., and Horton, M. A. (20 00) Adapting
atomic force microscopy for cell biology. Ultramicroscopy 82, 28 9 29 5.
14. Workman, ... F. (1989) Forces
in atomic force microscopy in air and water. Appl. Phys. Lett. 54, 26 51 26 53.
4. Butt, H J., Siedle, P., Seifert, K., et al. (1993) Scan speed lim...
Atomic Force Microscopy in Cell Biology Episode 1 Part 3 pdf
... needs to be tracked continuously.
3 .2. AFM Imaging and F-d Analysis
3 .2. 1.Imaging of Cells
3 .2. 1.1. FIXED OR DEHYDRATED CELLS
When a cell is fixed, through cross-linking of the plasma membrane ... view.
2. Tip contamination: effects and diagnostics. A cell cultured in a biofluid contains
proteins, cell debris, and other contaminants in solution. The probe tip will inevi-
tabl...
Atomic Force Microscopy in Cell Biology Episode 1 Part 5 doc
... parameters in the Scan Console follows:
Amplitude Reference -0.101
I-gain 0.177, P-gain 0.0488, A-gain 0, S-gain 0
when the maximum ADD value is around 5.
I-gain 0.05 92, P-gain 0.0 122 , A-gain 0, S-gain ... the image.
26 . Check the composition of the image by test scan.
27 . Write the sample information in the column for comments.
28 . Change the number of the scan points to 5 12 ×...
Atomic Force Microscopy in Cell Biology Episode 1 Part 6 pdf
... Grattarola, M. (1994) Scanning force microscopy on live cultured cells:
imaging and force- versus-distance investigations. J. Microscopy 176, 25 4 26 1.
2. Oberleithner, H., Brinckmann, E., Giebisch, ... local forces is proving to be a powerful tool for cell analy-
sis (1 ,2) . In contrast with electron microscopy observations in particular, AFM
improves biological studies in...