Atomic Force Microscopy in Cell Biology Episode 1 Part 8 pps

... Instrum. 71, 4 31 436. 15 . Radmacher, M., Cleveland, J. P., and Hansma, P. K. (19 95) Improvement of ther- mally induced bending of cantilevers used for atomic force microscopy. Scanning 17 , 11 7 12 1. ... al. (19 94) Tapping mode atomic force microscopy in liquids. Appl. Phys. Lett. 64, 17 38 17 40. 11 . Lantz, M., Liu, Y. Z., Cui, X. D., Tokumoto, H., and Lindsay, S. M....

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... 460, 29 2–300. 21 . Pang, G. K., Baba-Kishi, K. Z., and Patel, A. (20 00) Topographic and phase- contrast imaging in atomic force microscopy. Ultramicroscopy 81( 2) , 35–40. 22 . Butt, H-J. (19 91) Measuring ... Van Haesendonck, C. (19 91) Can atomic force microscopy tips be inspected by atomic force microscopy? J. Vac. Sci. Technol. B. 9, 13 09 13 12. 3. Keller, D. an...

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... needs to be tracked continuously. 3. 2. AFM Imaging and F-d Analysis 3. 2 .1. Imaging of Cells 3. 2 .1. 1. FIXED OR DEHYDRATED CELLS When a cell is fixed, through cross-linking of the plasma membrane ... skin fibroblasts and 3T3 cells are generally maintained as monolayer cultures in Dulbecco’s modified Eagles’ medium/Ham F12 con- taining 15 mM NaHCO 3 , 50 U/mL penicillin, 50 mg/m...

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... filament dynamics in living glial cells imaged by atomic force microscopy. Science 257, 19 44 19 46 . 3. Hoh, J. H. and Hansma, P. K. (19 92) Atomic force microscopy for high-resolu- tion imaging in cell biology. ... imaging of living fibroblasts using force modu- lation in AFM. J. Electron Microsc. 49 , 47 3 4 81. 26. Hellemans, L., Waeyaert, K., and Hennau, F. (...

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... High 12 8. 410 kHz Low 12 5. 410 kHz Gain 1. 000 Vib. Voltage 1. 51 1 V LPF 1. 0 kHz HPF 1. 0 kHz Time 5 s Vib. Freq. 12 6.800 Amplitude 1. 068 V Peak Freq. 12 6. 918 kHz ∆F 0.3 35 Q 379.273 Phase 56 .52 0° h. ... W. (19 96) Scanning force microscopy. Cosmet. Toilet .11 1, 57 – 65. 5. You, H. and Yu, L. (19 97) Atomic force microscopy as a tool for study of human hair...

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... monitored (some cell excrements in frame B are magnified in Fig. 6) . 11 6 Bischoff et al. 11 6 Fig. 6. Zooming in on frame B of Fig. 5 (same z scale for both figures). Increasing contrast in the deflection ... cells: imaging and force- versus-distance investigations. J. Microscopy 17 6, 254–2 61 . 2. Oberleithner, H., Brinckmann, E., Giebisch, G., and Geibel, J. (19...

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... 313 31- 028). 9. Fetal bovine serum (FBS; heat inactivated; Gibco 10 108 -15 7) . 10 . Horse serum (heat inactivated; Gibco 26050- 070 ). 11 . Poly-D-Lysine (Sigma P -72 80) or Poly-L-Lysine (Sigma P- 915 5). 12 . ... by atomic force microscopy. J. Cell Sci. 10 7, 11 05 11 14. 4. Ricci, D., Tedesco, M., and Grattarola, M. (19 97) Mechanical and morphological properties of livin...

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... 74, 11 79 11 84 . 5. Marshall, G. W., Jr., Balooch, M., Kinney, J. H., and Marshall, S. J. (19 95) Atomic force microscopy of conditioning agents on dentin. J. Biomed. Mater. Res. 29, 13 81 13 87 . 6. ... valuable in characterizing differences in dem- ineralization response with different solutions and with different types of den- AFM and Cardiac Physiology 16 1 16 1 12 Ap...

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... and Hansma, P. K. ( 19 90 ) Imaging cells with the atomic force microscope. J. Struct. Biol. 10 5, 54– 61. 18 . Kasas, S. and Ikai, A. ( 19 96 ) A method for anchoring round shaped cells for atomic force microscope ... J. 66, 17 17 17 25. 13 . Schoenberger, C. A. and Hoh, J. H., ( 19 94 ) Slow cellular dynamics in MDCK and R5 cells monitored by time-lapse atomic force mi...

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... daptomycin: atomic force microscopy investigation. Chemotherapy 14 , 336-3 41. 14 . Nagao, E. and Dvorak, J. A. (19 99) Developing the atomic force microscope for studies of living cells. Intern. ... January, 21 23. 15 . Schaus, S. S., and Henderson, E. R. (19 97) Cell viability and probe -cell mem- brane interactions of XR1 glial cells imaged by atomic force microscopy....

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... H ă orber I. Introduction 1 II. Scanning Tunneling Microscopy 4 III. Atomic Force Microscopy 7 IV. Force Spectroscopy 13 V. Photonic Force Microscopy 21 References 30 2. The Atomic Force Microscope in ... Studies Using the Atomic Force Microscope Peter Hinterdorfer I. Introduction 11 5 II. Experimental Approach 11 7 III. Dynamic Force Spectroscopy 12 4 IV. Re...

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... of rupture forces of biotin/avidin lead to forces of 600 pN. In AFM mea- surements done at the 10 0-ms time scale, the actual measured forces are between 10 0 and 20 0 pN (Florin et al., 19 94). A. ... structure that interacts with its light chain, binding to the cargo. Several studies on kinesins have shown that the neck and the first hinge region of the motor play important roles in...

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... Studies 49 Hăaberle, W., Hăorber, J. K. H., Ohnesorge, F., Smith, D. P. E., and Binnig, G. (19 92). In situ investigation of single living cell infected by viruses. Ultramicroscopy 42 44 , 11 61 11 67. Henderson, ... Sakaguchi, D. S. (19 92). Actin filament dynamics in living glial cells imaged by atomic force microscopy. Science 257, 19 44 19 46 . Hoh, J. H., and Hansma, P....

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... filaments, or determine the architecture of the network. Prominent examples of actin-binding pro- teins are α-actinin (bundler), gelsolin (severs filaments, binds to monomers), filamin (cross-links filaments), ... reserved. 00 91- 679X/02 $ 35. 00 67 4. Measuring Elastic Properties of Living Cells 85 Fig. 11 (continued ). and therefore it is capable of following biological processes in situ....

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... (2000). Affinity of trans-interacting VE-cadherin determined by atomic force microscopy. Single Mol. 1, 11 9 12 2. Binnig, G., Quate, C. F., and Gerber, Ch. (19 86 ). Atomic force microscope. Phys. Rev. ... (19 99). Single integrin molecule adhesion forces in intact cells measured by atomic force microscopy. Biochem. Biophys. Res. Commun. 259, 645–650. Lin, J T., Kormanec, J...

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