Atomic Force Microscopy in Cell Biology Episode 2 Part 2 ppt

... Surface Sci 460, 29 2–300. 21 . Pang, G. K., Baba-Kishi, K. Z., and Patel, A. (20 00) Topographic and phase- contrast imaging in atomic force microscopy. Ultramicroscopy 81 (2) , 35–40. 22 . Butt, H-J. ... by pulsed- force- mode atomic force microscopy. Ultramicroscopy 82, 20 3 21 2. 36 Ricci and Braga Low-pass filters, although capable of reducing noise in the data, will i...

Ngày tải lên: 06/08/2014, 02:20

... wide interest in biology because of their fun- damental functions in the cell. They are responsible, e.g., for both targeting organelles through the cells and setting up the mitotic spindle during ... structure that interacts with its light chain, binding to the cargo. Several studies on kinesins have shown that the neck and the first hinge region of the motor play important roles in...

Ngày tải lên: 06/08/2014, 02:20

... Rev. Sci. Instr. 70, 121 – 124 . 13. Lehenkari, P. P., Charras, G. T., Nykanen, A., and Horton, M. A. (20 00) Adapting atomic force microscopy for cell biology. Ultramicroscopy 82, 28 9 29 5. 14. Workman, ... F. (1989) Forces in atomic force microscopy in air and water. Appl. Phys. Lett. 54, 26 51 26 53. 4. Butt, H J., Siedle, P., Seifert, K., et al. (1993) Scan speed lim...

Ngày tải lên: 06/08/2014, 02:20

... at intervals of 1 2 µm using indents of submicro- scopic size (approx 300–500 nm). The spacing is needed to avoid influence of one indent on the adjacent indent (see discussion in ref. 23 ). Using ... surrounding intertubular dentin, leaving enlarged tubule lumens (Fig. 4B). Exposure increments are continued in steps of 10 s to a minute or more to follow continued changes in the denti...

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... de-adhesion forces were measured in developing cells in which additional cell adhesion proteins are expressed. Cells in the aggregation stage are distinguished from growth-phase cells by EDTA-stable cell ... line) leads to relaxation of the repulsive force. When ligand–receptor binding has occurred, an attractive force develops (unbind- ing event) in the retrace (z = 0 21 nm)...

Ngày tải lên: 06/08/2014, 02:20

... first to measure the bind- ing forces between integrin receptors in intact cells and Arg–Gly–Asp (RGD) amino acid sequence-containing extracellular matrix protein ligands. Using a modification of ... Analysis A. Binding Force Measurements on Intact Cells B. Binding Map Analysis C. Material Property Analysis D. Induced Strain Calculation E. Interfacing AFM Measurements with Finite Element Mo...

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... needs to be tracked continuously. 3 .2. AFM Imaging and F-d Analysis 3 .2. 1.Imaging of Cells 3 .2. 1.1. FIXED OR DEHYDRATED CELLS When a cell is fixed, through cross-linking of the plasma membrane ... view. 2. Tip contamination: effects and diagnostics. A cell cultured in a biofluid contains proteins, cell debris, and other contaminants in solution. The probe tip will inevi- tabl...

Ngày tải lên: 06/08/2014, 02:20

... filament dynamics in living glial cells imaged by atomic force microscopy. Science 25 7, 1944–1946. 3. Hoh, J. H. and Hansma, P. K. (19 92) Atomic force microscopy for high-resolu- tion imaging in cell biology. ... carcinoma cells by scanning force microscopy. J. Cell Sci. 107, 24 27 24 37. 7. Gibson, C. T., Watson, G. S., and Myhra, S. (1996) Determination of the spr...

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... parameters in the Scan Console follows: Amplitude Reference -0.101 I-gain 0.177, P-gain 0.0488, A-gain 0, S-gain 0 when the maximum ADD value is around 5. I-gain 0.05 92, P-gain 0.0 122 , A-gain 0, S-gain ... the image. 26 . Check the composition of the image by test scan. 27 . Write the sample information in the column for comments. 28 . Change the number of the scan points to 5 12 ×...

Ngày tải lên: 06/08/2014, 02:20

... resulted in diminished cell motion and improved the study. Well- resolved topographic information could be obtained. Zooming -in allows the recording of pictures with increasing detail. Especially in ... local forces is proving to be a powerful tool for cell analy- sis (1 ,2) . In contrast with electron microscopy observations in particular, AFM improves biological studies invo...

Ngày tải lên: 06/08/2014, 02:20