structural and functional organization of the synapse

... deciphering the structural and functional organization of the synapse. We have assembled leaders in the field of synapse biology to describe and distill the wonders and mysteries of the synapse. ... must occur within the tiny space of the synapse, the precise molecular organization of proteins, lipids, and membranes at the synapse is paramou...

Ngày tải lên: 06/07/2014, 15:30

... spectrophotometric methods. Because the enzymatic activity of PRTFDC1 towards hypoxanthine and guanine is only 0.26% and 0.09%, respectively, of the activity of HPRT, the role of the PRTFDC1 in purine metabolism ... strength [20]. In the present study, we report the structural and functional characterization of the human HPRT homolog PRTFDC1. To shed light on...

Ngày tải lên: 15/02/2014, 01:20

... parts of the N-terminal fragment and ⁄ or the second cassette; AB DC EF Fig. 5. Effects of point mutation of the WalkerA and B motifs of the AAA-cassettes of Pex6p on the morphological appearance of ... structures and mislocalization of peroxisomal matrix proteins to the cytosol. Here we confirm and extend earlier studies of these AAA-peroxins and give a f...

Ngày tải lên: 07/03/2014, 16:20

... glutamate and aspar- tate residues confined to the portion of the molecule involving a-helix A, the nearby loop, the first and last turns of helices E and F, and the C-terminus helices I and J. In ... b-strand, F, which protrudes from the core of the monomer and runs anti-parallel to the same strand of a second monomer, allowing for the formation of the...

Ngày tải lên: 06/03/2014, 00:21

... the loss of the self energy in the wild-type enzyme of the residue that was mutated, DG pairwise i;j ðWTÞ are the pair wise energies of the original resi- due ‘i’ in the native protein with the ... with the rest of the residues, DDG self k ðmutÞ are the changes of the self energies of the resi- dues ‘k’ that change either their ionization or conformation...

Ngày tải lên: 16/03/2014, 10:20

... substrate. The Val349Ile mutant of the 15S-COX formed 65% PGF 2a and 35% of the 15R-epimer of PGF 2a . In the case of the 15R-COX, the Ile349Val mutation caused a more pronounced effect on the stereochemistry ... residues lie outside of the active site and mostly on the surface of the catalytic globular domain of the COX protein. Protein expression and...

Ngày tải lên: 23/03/2014, 12:20

... independently of the presence of AREs (Fig. 6B, lanes 2 and 5). It is possible that in the region located before the AREs, consisting of 343 bp of the 3¢ UTR of the IL-1b transcript, there is a putative ... in the 3¢ UTR of IL-6 mRNA [4,17]. The presence of such a sequence may account for the degra- dation of both types of IL-1b transcripts, with and with...

Ngày tải lên: 18/02/2014, 14:20

... to the side chain of Asp70, and one from the backbone N of Gly63, and the phosphate group forming hydrogen bonds from O1 to Arg55, from O2 to the backbone N and the side chain of Thr138, and ... to the three lysines (Fig. 3E). There is no water molecule fix- ing the three lysines from the B, C and F subunits, and there is no direct interaction between subunits...

Ngày tải lên: 18/02/2014, 16:20

... analyses of the predicted amino acid sequence showed that the C-terminal half of the RepA of the pIT3 plasmid is sequence-similar to the helicases of the phage-encoded superfamily III pro- teins. The ... of 0.2 nm, and a bandwidth of 1 nm. Thermal stability of Rep245 was measured by incu- bating the protein at 60, 70 and 80 °C for 30 min and then recording...

Ngày tải lên: 18/02/2014, 18:20

... and then the energy minimization of the model was iterated 300 cycles using the conjugate gradient methods with the program DISCOVER _3 in INSIGHT II . To construct the dimeric form of LPL, the model ... on the crystal structures of the human, porcine, and horse PLs (Fig. 1). A frontal view illustrates the overall saddle shape of the dimeric structure and th...

Ngày tải lên: 21/02/2014, 15:20