Introduction to Modern Liquid Chromatography, Third Edition part 80 pptx
Introduction to Modern Liquid Chromatography, Third Edition part 80 pptx
... Preparative
and Non-linear Chromatography, 2nd ed., Academic Press, Boston, 2006.
5. J. H. Knox and H. M. Pyper, J. Chromatogr., 363 (1986) 1.
6. L.R.Snyder,G.B.Cox,andP.E.Antle,Chromatographia, 24 (1987) ... 15.13a is no longer sufficient to cause
the peaks to touch. That is, the divergent case allows a larger weight of injected
sample (other factors equal), compared to the equal-S ca...
Introduction to Modern Liquid Chromatography, Third Edition part 5 pptx
... most laboratories that deal with a need for analyzing chemical mixtures are
equipped for HPLC; it is often the first choice of technique
Introduction to Modern Liquid Chromatography, Third Edition, ... Do?, 4
1.2 A SHORT HISTORY OF HPLC, 6
1.3 SOME ALTERNATIVES TO HPLC, 8
1.3.1 Gas Chromatography (GC), 8
1.3.2 Thin-Layer Chromatography (TLC), 9
1.3.3 Supercritical Fluid Chromato...
Introduction to Modern Liquid Chromatography, Third Edition part 7 pptx
... Switching, 79
2.7.7 Retention Predictions Based on Solute Structure, 80
Introduction to Modern Liquid Chromatography, Third Edition, by Lloyd R. Snyder,
Joseph J. Kirkland, and John W. Dolan
Copyright ... Chrommatogr., 5 (1967) 183.
25. J. J. Kirkland, ed., Modern Practice of Liquid Chromatography, Wiley-Interscience, New
York, 1971.
26. T. Braumann, G. Weber, and L. H. G...
Introduction to Modern Liquid Chromatography, Third Edition part 20 pptx
... Quadrupoles and Ion Traps, 188
4.14.3 Other MS Detectors, 190
4.15 OTHER HYPHENATED DETECTORS, 191
Introduction to Modern Liquid Chromatography, Third Edition, by Lloyd R. Snyder,
Joseph J. Kirkland, ... 158
4.3 INTRODUCTION TO INDIVIDUAL DETECTORS, 160
4.4 UV-VISIBLE DETECTORS, 160
4.4.1 Fixed-Wavelength Detectors, 163
4.4.2 Variable-Wavelength Detectors, 164
4.4.3 Diode-Arr...
Introduction to Modern Liquid Chromatography, Third Edition part 26 pptx
... heating,
microparticles of silica spherical composit
e
Figure 5.10 Aggregation of microparticles to form totally porous particles.
212 THE COLUMN
and the particles are strengthened by sintering. Small particles ... sol particles. Small, silica-sol particles are used to prepare particles
with smaller pores; larger pores are formed from larger silica sol-particles. This
aggregation method c...
Introduction to Modern Liquid Chromatography, Third Edition part 32 pptx
... 5,5-diphenylhydantoin; 3, acetophenone; 4,
benzonitrile; 5, 5-phenylpentanol; 6,anisole;7, toluene; 8, cis-chalcone; 9, ethylbenzene; 10,
trans-chalcone. Conditions: 150 × 4.6-mm (5-μm) columns; 45% acetonitrile-water; ... 5,
3,4-dichloroaniline; 6, 2-nitrotoluene; 7, 3-nitrotoluene; 8, toluene; 9,3-nitro-o-xylene; 10,
4-nitro-m-xylene. Conditions: 250 × 4.6-mm (5-μm) C
8
column; acetonitrile...
Introduction to Modern Liquid Chromatography, Third Edition part 36 pptx
... to ±1.5 unit for higher concentrations of the
buffer). The buffer capacity of the mobile phase is proportional to buffer concentra-
tion, which typically falls within a range of 5 to 25 mM. To ... reader
may wish to skip this digression, proceed to Section 7.2.1.2, andreturntothepresent
section as needed.
We can define the ‘‘effective buffer capacity’’ of the mobile phase to mean...
Introduction to Modern Liquid Chromatography, Third Edition part 39 pptx
... sulfonate concen-
tration of 6 to 7 mM would provide maximum resolution of peaks 4 to 7 (placing
peak 5 midway between peaks 4 and 6, but without moving peak 7 too close to
peak 6).
Figure 7.15d–f ... C
8
column (3-μmparti-
cles); 15% methanol- buffer (pH-3.2); 35
◦
C; 2.0 mL/min. Chromatograms recreated from
data in [48].
338 IONIC SAMPLES: REVERSED-PHASE, ION-PAIR, AND ION-EXCHANGE CH...
Introduction to Modern Liquid Chromatography, Third Edition part 44 pptx
... required
to achieve the desired retention range of 1 ≤ k ≤ 10, as well as to take advantage of
solvent-strength selectivity.
At the conclusion of exploratory TLC studies with silica plates, we need
to ... often tolerate lower values of N (Section 15.4.1.1).
8.6 HYDROPHILIC INTERACTION CHROMATOGRAPHY
(HILIC)
Hydrophilic interaction chromatography (HILIC) can be regarded as normal-phase
c...
Introduction to Modern Liquid Chromatography, Third Edition part 46 pptx
...
i
ii
100
80
60
40
20
0
100
80
60
40
20
0
0 5 10 15 2
0
(min)
5/25/40/100% B
at 0/5/15/20min
t
G
= 20 min
= (0 .80 – 0.10) = 0.70
%B
0 5 10 15 20
(min)
10 -80% B
in 20 min
o
= 10% B ≡ 0.10
f
= 80% ... example, 5% acetonitrile/water in the A-reservoir and 95% acetonitrile/water in
the B-reservoir. For the latter example, a (nominal) 0–100% B gradient would then
correspond to 5–95% ac...
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