Introduction to Modern Liquid Chromatography, Third Edition part 80 pptx

Introduction to Modern Liquid Chromatography, Third Edition part 80 pptx

Introduction to Modern Liquid Chromatography, Third Edition part 80 pptx

... Preparative and Non-linear Chromatography, 2nd ed., Academic Press, Boston, 2006. 5. J. H. Knox and H. M. Pyper, J. Chromatogr., 363 (1986) 1. 6. L.R.Snyder,G.B.Cox,andP.E.Antle,Chromatographia, 24 (1987) ... 15.13a is no longer sufficient to cause the peaks to touch. That is, the divergent case allows a larger weight of injected sample (other factors equal), compared to the equal-S ca...
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Introduction to Modern Liquid Chromatography, Third Edition part 5 pptx

Introduction to Modern Liquid Chromatography, Third Edition part 5 pptx

... most laboratories that deal with a need for analyzing chemical mixtures are equipped for HPLC; it is often the first choice of technique Introduction to Modern Liquid Chromatography, Third Edition, ... Do?, 4 1.2 A SHORT HISTORY OF HPLC, 6 1.3 SOME ALTERNATIVES TO HPLC, 8 1.3.1 Gas Chromatography (GC), 8 1.3.2 Thin-Layer Chromatography (TLC), 9 1.3.3 Supercritical Fluid Chromato...
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Introduction to Modern Liquid Chromatography, Third Edition part 7 pptx

Introduction to Modern Liquid Chromatography, Third Edition part 7 pptx

... Switching, 79 2.7.7 Retention Predictions Based on Solute Structure, 80 Introduction to Modern Liquid Chromatography, Third Edition, by Lloyd R. Snyder, Joseph J. Kirkland, and John W. Dolan Copyright ... Chrommatogr., 5 (1967) 183. 25. J. J. Kirkland, ed., Modern Practice of Liquid Chromatography, Wiley-Interscience, New York, 1971. 26. T. Braumann, G. Weber, and L. H. G...
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Introduction to Modern Liquid Chromatography, Third Edition part 20 pptx

Introduction to Modern Liquid Chromatography, Third Edition part 20 pptx

... Quadrupoles and Ion Traps, 188 4.14.3 Other MS Detectors, 190 4.15 OTHER HYPHENATED DETECTORS, 191 Introduction to Modern Liquid Chromatography, Third Edition, by Lloyd R. Snyder, Joseph J. Kirkland, ... 158 4.3 INTRODUCTION TO INDIVIDUAL DETECTORS, 160 4.4 UV-VISIBLE DETECTORS, 160 4.4.1 Fixed-Wavelength Detectors, 163 4.4.2 Variable-Wavelength Detectors, 164 4.4.3 Diode-Arr...
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Introduction to Modern Liquid Chromatography, Third Edition part 26 pptx

Introduction to Modern Liquid Chromatography, Third Edition part 26 pptx

... heating, microparticles of silica spherical composit e Figure 5.10 Aggregation of microparticles to form totally porous particles. 212 THE COLUMN and the particles are strengthened by sintering. Small particles ... sol particles. Small, silica-sol particles are used to prepare particles with smaller pores; larger pores are formed from larger silica sol-particles. This aggregation method c...
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Introduction to Modern Liquid Chromatography, Third Edition part 32 pptx

Introduction to Modern Liquid Chromatography, Third Edition part 32 pptx

... 5,5-diphenylhydantoin; 3, acetophenone; 4, benzonitrile; 5, 5-phenylpentanol; 6,anisole;7, toluene; 8, cis-chalcone; 9, ethylbenzene; 10, trans-chalcone. Conditions: 150 × 4.6-mm (5-μm) columns; 45% acetonitrile-water; ... 5, 3,4-dichloroaniline; 6, 2-nitrotoluene; 7, 3-nitrotoluene; 8, toluene; 9,3-nitro-o-xylene; 10, 4-nitro-m-xylene. Conditions: 250 × 4.6-mm (5-μm) C 8 column; acetonitrile...
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Introduction to Modern Liquid Chromatography, Third Edition part 36 pptx

Introduction to Modern Liquid Chromatography, Third Edition part 36 pptx

... to ±1.5 unit for higher concentrations of the buffer). The buffer capacity of the mobile phase is proportional to buffer concentra- tion, which typically falls within a range of 5 to 25 mM. To ... reader may wish to skip this digression, proceed to Section 7.2.1.2, andreturntothepresent section as needed. We can define the ‘‘effective buffer capacity’’ of the mobile phase to mean...
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Introduction to Modern Liquid Chromatography, Third Edition part 39 pptx

Introduction to Modern Liquid Chromatography, Third Edition part 39 pptx

... sulfonate concen- tration of 6 to 7 mM would provide maximum resolution of peaks 4 to 7 (placing peak 5 midway between peaks 4 and 6, but without moving peak 7 too close to peak 6). Figure 7.15d–f ... C 8 column (3-μmparti- cles); 15% methanol- buffer (pH-3.2); 35 ◦ C; 2.0 mL/min. Chromatograms recreated from data in [48]. 338 IONIC SAMPLES: REVERSED-PHASE, ION-PAIR, AND ION-EXCHANGE CH...
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Introduction to Modern Liquid Chromatography, Third Edition part 44 pptx

Introduction to Modern Liquid Chromatography, Third Edition part 44 pptx

... required to achieve the desired retention range of 1 ≤ k ≤ 10, as well as to take advantage of solvent-strength selectivity. At the conclusion of exploratory TLC studies with silica plates, we need to ... often tolerate lower values of N (Section 15.4.1.1). 8.6 HYDROPHILIC INTERACTION CHROMATOGRAPHY (HILIC) Hydrophilic interaction chromatography (HILIC) can be regarded as normal-phase c...
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Introduction to Modern Liquid Chromatography, Third Edition part 46 pptx

Introduction to Modern Liquid Chromatography, Third Edition part 46 pptx

... i ii 100 80 60 40 20 0 100 80 60 40 20 0 0 5 10 15 2 0 (min) 5/25/40/100% B at 0/5/15/20min t G = 20 min = (0 .80 – 0.10) = 0.70 %B 0 5 10 15 20 (min) 10 -80% B in 20 min o = 10% B ≡ 0.10 f = 80% ... example, 5% acetonitrile/water in the A-reservoir and 95% acetonitrile/water in the B-reservoir. For the latter example, a (nominal) 0–100% B gradient would then correspond to 5–95% ac...
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