Introduction to Modern Liquid Chromatography, Third Edition part 62 ppt
Introduction to Modern Liquid Chromatography, Third Edition part 62 ppt
... Chromatography, 625
13.6.2 Ion-Moderated Partition Chromatography, 626
13.6.3 High-Performance Anion-Exchange Chromatography, 628
Introduction to Modern Liquid Chromatography, Third Edition, by Lloyd ... Anion-Exchange Chromatography, 619
13.5.2 Reversed-Phase Chromatography, 620
13.5.3 Hydrophobic Interaction Chromatography, 624
13.6 SEPARATION OF CARBOHYDRA...
Introduction to Modern Liquid Chromatography, Third Edition part 5 pptx
... most laboratories that deal with a need for analyzing chemical mixtures are
equipped for HPLC; it is often the first choice of technique
Introduction to Modern Liquid Chromatography, Third Edition, ... Do?, 4
1.2 A SHORT HISTORY OF HPLC, 6
1.3 SOME ALTERNATIVES TO HPLC, 8
1.3.1 Gas Chromatography (GC), 8
1.3.2 Thin-Layer Chromatography (TLC), 9
1.3.3 Supercritical Fluid Chromato...
Introduction to Modern Liquid Chromatography, Third Edition part 7 pptx
... Switching, 79
2.7.7 Retention Predictions Based on Solute Structure, 80
Introduction to Modern Liquid Chromatography, Third Edition, by Lloyd R. Snyder,
Joseph J. Kirkland, and John W. Dolan
Copyright ... Chrommatogr., 5 (1967) 183.
25. J. J. Kirkland, ed., Modern Practice of Liquid Chromatography, Wiley-Interscience, New
York, 1971.
26. T. Braumann, G. Weber, and L. H. Gri...
Introduction to Modern Liquid Chromatography, Third Edition part 9 ppt
... through detector +
connecting tubing
(extra-column peak broadenin
g
)
(e)(f )
1
2
3
4
5
6
7
9
8
2
3
1
2
3
4
5
6
8
7
9
Detector
particle
pore
Figure 2.12 Illustration of various contributions to band ... increase in proportion
to retention time. A continual increase in peak width from the beginning to the end
of the chromatogram is therefore observed; for example, see the chromatogram o...
Introduction to Modern Liquid Chromatography, Third Edition part 10 ppt
... R
s
≥ 2), normally corresponding to a one -third
safety factor (vs. baseline separation with R
s
≈ 1.5, for peaks of comparable size).
The goal of R
s
≥ 2 takes into account minor peak tailing, ... choice of column length, particle size, and
flow rate will always correspond to a minimum value of h ≈ 2(orν ≈ 3). Because
commercial columns are limited to certain lengths and particle sizes...
Introduction to Modern Liquid Chromatography, Third Edition part 11 ppt
... laboratories have preferred columns packed with -5μm
particles. Such columns are less demanding in terms of equipment (Section 3.9), and
are less likely to be plugged by particulates. Today, ... by
conventional HPLC systems (with maximums of 6000 psi or 400 bar) is referred to
as ultra–high-pressure liquid chromatography, or U-HPLC. U-HPLC, with pressures
>
6000 psi can be used for...
Introduction to Modern Liquid Chromatography, Third Edition part 18 ppt
... a graphical chromatogram for a visual inspection of the
quality of the data. As laboratories move to paperless records, the chromatogram
may be viewed only on a computer monitor. For qualitative ... result, when
a regulatory auditor reviews the data. It is expected that as additional regulatory
requirements are placed on the HPLC laboratory, software manufacturers will
continue to provide...
Introduction to Modern Liquid Chromatography, Third Edition part 20 pptx
... Quadrupoles and Ion Traps, 188
4.14.3 Other MS Detectors, 190
4.15 OTHER HYPHENATED DETECTORS, 191
Introduction to Modern Liquid Chromatography, Third Edition, by Lloyd R. Snyder,
Joseph J. Kirkland, ... 158
4.3 INTRODUCTION TO INDIVIDUAL DETECTORS, 160
4.4 UV-VISIBLE DETECTORS, 160
4.4.1 Fixed-Wavelength Detectors, 163
4.4.2 Variable-Wavelength Detectors, 164
4.4.3 Diode-Arr...
Introduction to Modern Liquid Chromatography, Third Edition part 23 ppt
... cell
dual
photodiode
detector
output
(a)
photodiode
arra
y
(b)
Figure 4.22 Schematic of a deflection refractive index (RI) detector. (a) Dual-photodiode
detector; (b) photodiode array detector (lamp and flow ... heat-exchanger required
Sensitive to temperature changes
Reliable, fairly easy to operate
Nondestructive
of photodiode-array technology to the RI detector allows multiple photodio...
Introduction to Modern Liquid Chromatography, Third Edition part 24 ppt
... detector response to reagents
to reduce extra-column peak broadening during reaction is to use a knitted reactor,
where narrow-bore flexible tubing is knitted into a series of tight radius bends to
create ... fast reaction is desired to
minimize the reactor volume requirements. Also reactions that go to completion tend
to be more reproducible than those that only go partially to...
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