Introduction to Modern Liquid Chromatography, Third Edition part 48 pps
Introduction to Modern Liquid Chromatography, Third Edition part 48 pps
... conditions
that affect k*. The reader may choose to skip to Section 9.3, and return to this
section at a later time—or as needed. However, this treatment can add to the
reader’s intuitive understanding ... increase in gradient time from 5to2 0
minutes (Fig. 9.13b), corresponding to an increase in average k
∗
from 5 to 20. As a
result the retention of peak 2 relative to that of p...
Introduction to Modern Liquid Chromatography, Third Edition part 3 ppsx
... RPC-
5
Chromatography, 623
13.5.3 Hydrophobic Interaction
Chromatography, 624
13.6 Separation of Carbohydrates, 625
13.6.1 Hydrophilic Interaction
Chromatography, 625
13.6.2 Ion-Moderated Partition
Chromatography, ... 648
13.10 Synthetic Polymers, 648
13.10.1 Background, 648
13.10.2 Techniques for Polymer Analysis, 651
13.10.3 Liquid- Chromatography Modes for Polymer
Analysis, 653...
Introduction to Modern Liquid Chromatography, Third Edition part 6 pps
... the early days of HPLC the technique was sometimes referred to
as ‘‘high-pressure liquid chromatography’’ or ‘‘high-speed liquid chromatography, ’
for reasons suggested by Figure 1.5g. Figure 1.5h ... liquid liquid partition chromatography that was
later improved in various ways. HPLC with a liquid stationary phase was since
replaced by bonded-phase HPLC, the use of CCC as an alter...
Introduction to Modern Liquid Chromatography, Third Edition part 13 ppsx
... Snyder and J. J. Kirkland, Introduction to Modern Liquid Chromatography, 2nd
ed., Wiley-Interscience, New York, 1979, pp. 34–36.
46. Reviewer Guidance. Validation of Chromatographic Methods, Center ... Chromatogr. A, 1119 (2006) 140.
22. J. C. Giddings, Dynamics of Chromatography, Part 1, Principles and Theory, Dekker,
New York, 1965.
23. G. Guiochon, in High-Performance Liquid...
Introduction to Modern Liquid Chromatography, Third Edition part 14 pps
... 130
3.8.5 Report Generation, 130
3.8.6 Regulatory Functions, 130
3.9 EXTRA-COLUMN EFFECTS, 131
Introduction to Modern Liquid Chromatography, Third Edition, by Lloyd R. Snyder,
Joseph J. Kirkland, ... the pump. The frit acts as a weight to keep the inlet tubing at the
bottom of the reservoir, but its primary function is to provide backup filtration to
remove particulate matte...
Introduction to Modern Liquid Chromatography, Third Edition part 33 pps
... developed, it is likely to be used over an extended period and in different
laboratories. Columns degrade during routine use and must therefore be replaced
from time to time. As discussed in ... has lim-
ited practical application. The reader may therefore wish to skip to following
Section 6.3.6.
284 REVERSED-PHASE CHROMATOGRAPHY FOR NEUTRAL SAMPLES
0 10203040
Original (primary) metho...
Introduction to Modern Liquid Chromatography, Third Edition part 34 ppsx
... in flow rate
from 2.0 to 1.0 mL/min (not shown) results in an insignificant increase in resolution
(to R
s
= 1.2), while run time is doubled to 10 min. Alternatively, the particle size
can be reduced, ... (called
‘‘phase-optimized liquid chromatography’’ or POPLC
®
from Bischoff). Experiments
with individual columns define sample retention for each column type, and it is then
possible t...
Introduction to Modern Liquid Chromatography, Third Edition part 40 pps
... the analysis of any mixture that contained organic acids or bases. Together
with liquid liquid partition and adsorption chromatography, IEC then accounted
for most of the reported separations ... CHROMATOGRAPHY (IEC)
Ion-exchange chromatography (IEC) is an important separation technique, but for
the most part, today, with limited applications:
• mixtures of inorganic ions (ion chromatogr...
Introduction to Modern Liquid Chromatography, Third Edition part 41 pps
... chromatography
(NPC). Another term used to describe NPC is adsorption chromatography,in
recognition of the fact that retained solute molecules are attached to (or adsorbed
onto) the surface of particles ... standards by anion-exchange chro-
matography with amperometric detection. Sample: 1, myo-inositol; 2,
D-sorbitol; 3, lactitol;
4,
L-fructose; 5, rhamnose; 6, D-galactose; 7, D-glucosam...
Introduction to Modern Liquid Chromatography, Third Edition part 43 pps
... affected by which isomer can better position itself adjacent to the
surface, so as to allow each polar methoxy group in the molecule to interact
with an adjacent silanol group on the surface. In ... silica
columns—compared to polar-bonded-phase columns. Consequently a change from
silica to a polar-bonded-phase column, with further variation of the mobile phase
(change in %B and/or B...
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