Introduction to Modern Liquid Chromatography, Third Edition part 36 pptx
Introduction to Modern Liquid Chromatography, Third Edition part 36 pptx
... to ±1.5 unit for higher concentrations of the
buffer). The buffer capacity of the mobile phase is proportional to buffer concentra-
tion, which typically falls within a range of 5 to 25 mM. To ... reader
may wish to skip this digression, proceed to Section 7.2.1.2, andreturntothepresent
section as needed.
We can define the ‘‘effective buffer capacity’’ of the mobile phase to mean...
Introduction to Modern Liquid Chromatography, Third Edition part 5 pptx
... most laboratories that deal with a need for analyzing chemical mixtures are
equipped for HPLC; it is often the first choice of technique
Introduction to Modern Liquid Chromatography, Third Edition, ... Do?, 4
1.2 A SHORT HISTORY OF HPLC, 6
1.3 SOME ALTERNATIVES TO HPLC, 8
1.3.1 Gas Chromatography (GC), 8
1.3.2 Thin-Layer Chromatography (TLC), 9
1.3.3 Supercritical Fluid Chromato...
Introduction to Modern Liquid Chromatography, Third Edition part 7 pptx
... Switching, 79
2.7.7 Retention Predictions Based on Solute Structure, 80
Introduction to Modern Liquid Chromatography, Third Edition, by Lloyd R. Snyder,
Joseph J. Kirkland, and John W. Dolan
Copyright ... Chrommatogr., 5 (1967) 183.
25. J. J. Kirkland, ed., Modern Practice of Liquid Chromatography, Wiley-Interscience, New
York, 1971.
26. T. Braumann, G. Weber, and L. H. Gri...
Introduction to Modern Liquid Chromatography, Third Edition part 20 pptx
... Quadrupoles and Ion Traps, 188
4.14.3 Other MS Detectors, 190
4.15 OTHER HYPHENATED DETECTORS, 191
Introduction to Modern Liquid Chromatography, Third Edition, by Lloyd R. Snyder,
Joseph J. Kirkland, ... 158
4.3 INTRODUCTION TO INDIVIDUAL DETECTORS, 160
4.4 UV-VISIBLE DETECTORS, 160
4.4.1 Fixed-Wavelength Detectors, 163
4.4.2 Variable-Wavelength Detectors, 164
4.4.3 Diode-Arr...
Introduction to Modern Liquid Chromatography, Third Edition part 26 pptx
... heating,
microparticles of silica spherical composit
e
Figure 5.10 Aggregation of microparticles to form totally porous particles.
212 THE COLUMN
and the particles are strengthened by sintering. Small particles ... sol particles. Small, silica-sol particles are used to prepare particles
with smaller pores; larger pores are formed from larger silica sol-particles. This
aggregation method c...
Introduction to Modern Liquid Chromatography, Third Edition part 32 pptx
... 5,5-diphenylhydantoin; 3, acetophenone; 4,
benzonitrile; 5, 5-phenylpentanol; 6,anisole;7, toluene; 8, cis-chalcone; 9, ethylbenzene; 10,
trans-chalcone. Conditions: 150 × 4.6-mm (5-μm) columns; 45% acetonitrile-water; ... 5,
3,4-dichloroaniline; 6, 2-nitrotoluene; 7, 3-nitrotoluene; 8, toluene; 9,3-nitro-o-xylene; 10,
4-nitro-m-xylene. Conditions: 250 × 4.6-mm (5-μm) C
8
column; acetonitrile...
Introduction to Modern Liquid Chromatography, Third Edition part 39 pptx
... sulfonate concen-
tration of 6 to 7 mM would provide maximum resolution of peaks 4 to 7 (placing
peak 5 midway between peaks 4 and 6, but without moving peak 7 too close to
peak 6).
Figure 7.15d–f ... C
8
column (3-μmparti-
cles); 15% methanol- buffer (pH-3.2); 35
◦
C; 2.0 mL/min. Chromatograms recreated from
data in [48].
338 IONIC SAMPLES: REVERSED-PHASE, ION-PAIR, AND ION-EXCHANGE CH...
Introduction to Modern Liquid Chromatography, Third Edition part 44 pptx
... required
to achieve the desired retention range of 1 ≤ k ≤ 10, as well as to take advantage of
solvent-strength selectivity.
At the conclusion of exploratory TLC studies with silica plates, we need
to ... often tolerate lower values of N (Section 15.4.1.1).
8.6 HYDROPHILIC INTERACTION CHROMATOGRAPHY
(HILIC)
Hydrophilic interaction chromatography (HILIC) can be regarded as normal-phase
c...
Introduction to Modern Liquid Chromatography, Third Edition part 46 pptx
... behavior can make it
extremely difficult to obtain reproducible isocratic separations of macromolecules in
different laboratories, or even within the same laboratory. Furthermore the isocratic
separation ... separations by RPC (with fixed run times,
for automated analysis) are only practical by means of gradient elution and are
commonly used to assay combinatorial libraries [3], as well as...
Introduction to Modern Liquid Chromatography, Third Edition part 57 pptx
... need to be
applied to this value before the final sample concentration is reported.
11.4 QUANTITATIVE ANALYSIS 521
it in water or buffer to make a primary stock. This stock then is added to the ... Detector (CLND)
The CLND (Section 4.9) responds to the nitrogen content of the analyte. Because the
detector response is proportional to the molar content of nitrogen in the sample, the...
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