Introduction to Modern Liquid Chromatography, Third Edition part 17 pdf
Introduction to Modern Liquid Chromatography, Third Edition part 17 pdf
... cycle time to just a few seconds. Three common autosampler designs are in common use: • pull -to- fill • push -to- fill • needle-in-loop 3.6.2.1 Pull -to- Fill Autosamplers The pull -to- fill autosampler ... detector. One way to avoid this is to use a switching valve to divert to waste the portion of the column effluent that contains most of the protein—usually the early portion of the...
Ngày tải lên: 04/07/2014, 01:20
Introduction to Modern Liquid Chromatography, Third Edition part 95 pdf
... rules, 583–584 structure vs. chromatography, 583–584 Proteomics, multidimensional liquid chromatography, 616–618 Protocol; see Validation, protocol Pseudocritical chromatography, 654 Pump(s), 104–113 active ... U-HPLC High-pressure liquid chromatography, 8 High-priced liquid chromatography; see U-HPLC High-speed liquid chromatography, 8 HILIC, see Hydrophilic interaction chr...
Ngày tải lên: 04/07/2014, 01:20
Introduction to Modern Liquid Chromatography, Third Edition part 1 pdf
... Hybrid Systems, 111 INTRODUCTION TO MODERN LIQUID CHROMATOGRAPHY Third Edition LLOYD R. SNYDER LC Resources, Inc. Orinda, CA JOSEPH J. KIRKLAND Advanced Materials Technology Wilmington, DE JOHN W. ... Lloyd R. Introduction to modern liquid chromatography / Lloyd R. Snyder, Joseph J. Kirkland. – 3rd ed. / John W. Dolan. p. cm. Includes index. ISBN 978-0-470-16754-0 (cloth)...
Ngày tải lên: 04/07/2014, 01:20
Introduction to Modern Liquid Chromatography, Third Edition part 4 pdf
... Leaks, 825 17. 4.1.4 Autosampler Leaks, 825 17. 4.1.5 Column Leaks, 828 17. 4.1.6 Detector Leaks, 828 17. 4.2 Abnormal Pressure, 830 17. 4.2.1 Pressure Too High, 831 17. 4.2.2 Pressure Too Low, 832 17. 4.2.3 ... 835 17. 4.3.5 Temperature Problems, 836 17. 4.3.6 Retention-Problem Symptoms, 836 17. 4.4 Peak Area, 838 17. 4.4.1 Peak Area Too Large, 839 17. 4.4.2 Peak Area Too Small,...
Ngày tải lên: 04/07/2014, 01:20
Introduction to Modern Liquid Chromatography, Third Edition part 16 pdf
... Accumulator-Piston Pumps An alternative design for the dual-piston pump is the accumulator-piston, or tandem-piston design shown in Figure 3.13d. In this case, one piston feeds into the other piston ... are connected to opposite ends of the loop, and the pump is connected to the column. To inject, the rotor is moved to the inject position (3.17b), and the contents of the loop are swep...
Ngày tải lên: 04/07/2014, 01:20
Introduction to Modern Liquid Chromatography, Third Edition part 19 pdf
... operator’s manual, replacement of the needle seal every six months will help to avoid problems. The autosampler is the component of the HPLC system that is most likely to leak; it is wise to inspect ... proportioning-manifold replacement, injector rotor, detector lamp, tubing and fittings Factory-trained Proportioning-manifold repair, electronics, repairs involving module subassemblies,...
Ngày tải lên: 04/07/2014, 01:20
Introduction to Modern Liquid Chromatography, Third Edition part 21 pdf
... magnetic resonance Radioactivity Conductivity Chemiluminescent nitrogen Chiral 4.3 INTRODUCTION TO INDIVIDUAL DETECTORS The remainder of this chapter (Sections 4.4–4.16) provides a discussion of most HPLC detectors in use today. In Table 4.4, detectors are grouped ... be found in [16, 17] . 4.4 UV-VISIBLE DETECTORS The most widely used detectors in modern HPLC are photometers based on...
Ngày tải lên: 04/07/2014, 01:20
Introduction to Modern Liquid Chromatography, Third Edition part 31 pdf
... the interface between the stationary and mobile phases. Partition (Fig. 6.4c) considers the stationary phase to be similar to a liquid phase, into which the solute molecule dissolves. Notice that ... back onto or into the stationary phase—rather than extending into the mobile phase as shown. Presumably there is a decreased interaction with the column for solute molecules that are too lo...
Ngày tải lên: 04/07/2014, 01:20
Introduction to Modern Liquid Chromatography, Third Edition part 52 pdf
... software that facilitates method development. In this chapter we will Introduction to Modern Liquid Chromatography, Third Edition, by Lloyd R. Snyder, Joseph J. Kirkland, and John W. Dolan Copyright ... Waeghe, J. Chromatogr., 803 (1998) 1. 43. A. P. Schellinger, D. R. Stoll, and P. W. Carr, J. Chromatogr. A, 1064 (2005) 143. 44. A. P. Schellinger, D. R. Stoll, and P. W. Carr, J....
Ngày tải lên: 04/07/2014, 01:20
Introduction to Modern Liquid Chromatography, Third Edition part 65 pdf
... selectivity relative to other modes of col- umn chromatography. Three other chromatographic techniques (chromatofocusing, hydroxyapaptite chromatography, and immobilized-metal affinity chromatography; Sections ... point, binding to an anion exchanger below the protein pI, or binding to a cation exchanger above the pI. Similarly a protein may fail to bind to an anion exchanger above...
Ngày tải lên: 04/07/2014, 01:20