Introduction to Modern Liquid Chromatography, Third Edition part 13 ppsx

... Snyder and J. J. Kirkland, Introduction to Modern Liquid Chromatography, 2nd ed., Wiley-Interscience, New York, 1979, pp. 34–36. 46. Reviewer Guidance. Validation of Chromatographic Methods, Center ... Chromatogr. A, 1119 (2006) 140. 22. J. C. Giddings, Dynamics of Chromatography, Part 1, Principles and Theory, Dekker, New York, 1965. 23. G. Guiochon, in High-Performance Liquid...

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... 624 13. 6 Separation of Carbohydrates, 625 13. 6.1 Hydrophilic Interaction Chromatography, 625 13. 6.2 Ion-Moderated Partition Chromatography, 626 13. 6.3 High-Performance Anion-Exchange Chromatography, ... Size-Exclusion Chromatography, 653 13. 10.3.2 Interactive Liquid Chromatography, 653 13. 10.3.3 Liquid Chromatography under Critical Conditions, 655 13. 10.3.4 Other T...

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... in flow rate from 2.0 to 1.0 mL/min (not shown) results in an insignificant increase in resolution (to R s = 1.2), while run time is doubled to 10 min. Alternatively, the particle size can be reduced, ... (called ‘‘phase-optimized liquid chromatography’’ or POPLC ® from Bischoff). Experiments with individual columns define sample retention for each column type, and it is then possible t...

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... Section 9.2.2.5: (1) to clean the column between sample injections, (2) to shorten run time, or (3) to improve separation by adjusting selectivity for different parts of the chromatogram. Because ... gradient time to 13 minutes, which then requires an increase in final-%B to maintain k ∗ constant. As t G /Δφ = 31.6 for the ‘‘optimized’’ separation of Figure 9.18e, the new value of...

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... Da); 49% acetonitrile-pH-2.3 buffer. Note the column-packing pore-diameter at top of each figure; ‘‘ligand coverage’’ (x-axis) is proportional to surface area. Adapted from [10]. 13. 3.1.2 Particle ... inflammatory responses, and their introduction into drug products destined for human use must be avoided. 13. 3.1.4 Recovery of Mass and Biological Activity In applications where HPLC is us...

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... (TED) N CO O Me O N OH 2 O Figure 13. 20 Structure of two common IMAC ligands. Adapted from [55]. 13. 4 SEPARATION OF PEPTIDES AND PROTEINS 613 13.4.4 Hydrophilic Interaction Chromatography (HILIC) Water-soluble ... volatile, however, TFA is notorious for causing suppression in electrospray ionization [17]. 13. 4.4.3 Application of HILIC to Peptides and Proteins HILIC has been used succ...

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... found to be powerful chiral selectors with complementary enantioselectivity (different enantioselectivities and elution orders), leading to CSPs based on vancomycin [111], teicoplanin [112], ristocetin ... antibiotic columns, exemplified by enantiomer separations of Z-Ala (top) and 5-methyl-5-phenylhydantoin (bottom). Experimental conditions: Ristocetin A, 20:80 methanol/0.1% triethylammon...

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... as acetonitrile or acetone to a homogenized portion of a vegetable sample allows the extraction of pesticides into the solvent (step 2 of Fig. 16.7). Subsequent addition of salt (step 3) leads to ... extractors and thimbles are available for mg-size samples. The analyte concentration, the necessary mass to obtain a representative sample, and the chromatographic detector sensitivity, tog...

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... screwdriver or other metal tool, to avoid damaging the pump head. Do not use the piston to pry out the seal because the piston is likely to break. As you remove the seal, be sure to note how the seal ... sapphire piston. • Inspect the piston for any scratches or defects. A simple way to highlight any problems is to hold a laser pointer up to the end of the piston, which will cau...

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... (arrow). counter to automatically record the number of injection cycles, so as to allow the remaining rotor seal life to be estimated. When viewed in cross section, the grooves of the rotor shown in ... split and distorted peaks in Section 17.4.5.3); otherwise, it may be necessary to replace the column. Before putting the system back into service, consider if steps need to be taken...

Ngày tải lên: 04/07/2014, 01:20