Introduction to Modern Liquid Chromatography, Third Edition part 10 ppt

... SEPARATION 10 6 10 5 10 4 10 3 10 −1 10 −3 10 −2 10 −4 0.1 1 10 1000 N t 0 (min) d p = 10 mm (a) (b) 5 mm 2 mm 5 mm 2 mm 1 t 0 /N (sec) N 10 6 10 5 10 4 10 3 10 2 d p = 10 mm P = 2000 psi t 0 = 100 0 ... chromatograms during method development. Numerical values of 50 BASIC CONCEPTS AND THE CONTROL OF SEPARATION N = 10 6 10 5 10 4 10 3 100 10 1 N =...

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... most laboratories that deal with a need for analyzing chemical mixtures are equipped for HPLC; it is often the first choice of technique Introduction to Modern Liquid Chromatography, Third Edition, ... A SHORT HISTORY OF HPLC, 6 1.3 SOME ALTERNATIVES TO HPLC, 8 1.3.1 Gas Chromatography (GC), 8 1.3.2 Thin-Layer Chromatography (TLC), 9 1.3.3 Supercritical Fluid Chromatography (SFC...

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... Based on Solute Structure, 80 Introduction to Modern Liquid Chromatography, Third Edition, by Lloyd R. Snyder, Joseph J. Kirkland, and John W. Dolan Copyright © 2 010 John Wiley & Sons, Inc. 19 ... Chrommatogr., 5 (1967) 183. 25. J. J. Kirkland, ed., Modern Practice of Liquid Chromatography, Wiley-Interscience, New York, 1971. 26. T. Braumann, G. Weber, and L. H. Grim...

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... SEPARATION P (psi) = 2K 5K 15K 10 5 10 4 10 3 10 2 d p = 10 μm 0.1 1.0 10 100 F (mL/min) N (a) (b) 150 × 4.6-mm columns 15,000 10, 000 5,000 N 0123456 F ( mL/min ) 80°C, 200 Da 100 × 4.6-mm, 3-μm column 30°C, 6,000 ... cell) add to the total pressure measured at the pump outlet, but the sum of these contributions is usually minor (10 20%) compared to values of P from Equatio...

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... laboratories have preferred columns packed with -5μm particles. Such columns are less demanding in terms of equipment (Section 3.9), and are less likely to be plugged by particulates. Today, ... peak heights are normalized to 100 % for tallest peak in each chromatogram. Simulated chro- matograms based on data of [50, 51]. changes in both %B and temperature, it is possible to achieve a...

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... outlined in Sections 3 .10. 1.2 and 3 .10. 1.3 may be used to compare the test results to the manufacturer’s specifications (gener- ally found in the back of one or more of the operator’s manuals). The ... controller is set to deliver a series of solvent mixtures in a stair-step design. A good choice for this test is to use a 10% step size, so that mixtures of 0, 10, 20, , 80, 90, 1...

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... Detectors, 190 4.15 OTHER HYPHENATED DETECTORS, 191 Introduction to Modern Liquid Chromatography, Third Edition, by Lloyd R. Snyder, Joseph J. Kirkland, and John W. Dolan Copyright © 2 010 John ... on detector noise. Raw detector signal with ≈16 mAU short-term noise; 1-sec time constant reduces noise to <1mAU. 012345 Time (min) 0 −5 10 −15 5 10 15 0 −5 10 −15 5 10 1...

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... cell dual photodiode detector output (a) photodiode arra y (b) Figure 4.22 Schematic of a deflection refractive index (RI) detector. (a) Dual-photodiode detector; (b) photodiode array detector (lamp and flow ... heat-exchanger required Sensitive to temperature changes Reliable, fairly easy to operate Nondestructive of photodiode-array technology to the RI detector allows multiple photodio...

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... atmospheric pressure (760 torr) to 10 −5 to 10 −6 torr within the 5 to 10 cm flow path of the interface. Pressure is reduced by pumping most of the vaporized sample and mobile phase to waste (no concentration ... detector response to reagents to reduce extra-column peak broadening during reaction is to use a knitted reactor, where narrow-bore flexible tubing is knitted into...

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... connected to a network of smaller (e.g., 30- to 100 -nm) pores. By comparison, most other particles have pore diameters of 8 to 30 nm (diffusive pores). At high flow rates, solutes are carried into and ... J. N. Seiber, Anal. Chem., 49 (1977) 326. 11. L. R. Snyder and J. J. Kirkland, Introduction to Modern Liquid Chromatography,1 st ed., Wiley, New York, 1979, p. 136. 12. J. W....

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