Handbook of Microbiological Media, Fourth Edition part 203 pot

... species. 644 Rhynchosporium secalis, 206 Rhyzopltydium species, 980 Riboflavin assay, 1512 Riboflavin Assay Medium, 1512 Riboflavin Medium, 1512 Ribose Production Medium, 1513 Rice I'xlmcl ... 1600 Spirillum gracile Broth, 1601 Spirillum gracile Medium, 1601 Spirillum leptoferum, 1601 Spirillum lipoferum Medium. 1601 Spirillum Medium, 1602 Spirillum Nitrogen-Fixing Medium,...

Ngày tải lên: 03/07/2014, 18:21

... Acid: Filter sterilize 40.0mL of acetic acid using a teflon filter. Preparation of Ethanol: Filter sterilize 30.0mL of ethanol using a teflon filter. Preparation of Medium: Add components, except ... anaerobically add 0.1mL of sterile glucose, 0.1mL of sterile L-cysteine·HCl·H 2 O solution, and 0.1mL of sterile Na 2 S·9H 2 O solution to each tube. Mix thoroughly. Use: For th...

Ngày tải lên: 03/07/2014, 18:20

... of Acid Fuchsin Solution: Add Acid Fuchsin to 50.0mL of 5% aqueous dioxan. Mix well to dissolve. Caution: Acid Fuchsin is a potential carcinogen and care must be tak- en to avoid inhalation of ... for 15 min at 15 psi pressure–121°C. Aseptically add 0.6mL of sterile raffinose solution, 0.2mL of sterile Na 2 CO 3 solution, and 0.2mL of sterile CoCl 2 ·6H 2 O solution to each tube....

Ngày tải lên: 03/07/2014, 18:20

... of Medium: Aseptically combine 900.0mL of solu- tion A with 100.0mL of solution B. Mix thoroughly. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation and maintenance of ... elements solution 20.0mL Preparation of Base Solution: Sparge 2.0L of distilled/deionized water with 100% N 2 to remove O 2 . Add components to 2000.0mL of O 2 - free distilled/deioni...

Ngày tải lên: 03/07/2014, 18:20

... to 45°–50°C. Preparation of Medium: To 1.0L of sterile Eagle’s basal medium, aseptically add 1.0L of sterile, cooled agarose solution and 100.0mL of fetal calf serum. Mix thoroughly. Pour into ... per 100.0mL: Tris[hydroxymethyl]aminomethane buffer 25.0g Preparation of Tris Buffer Solution: Add 25.0g of tris to 65.0mL of distilled/deionized water. Titrate to pH 7.6–7.7 with con...

Ngày tải lên: 03/07/2014, 18:20

... 100.0mL of sterile vitamin B 12 solution. Mix thoroughly. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation and maintenance of Synechococcus species. For the isolation of ... 100.0mL of sterile vitamin B 12 solution. Mix thoroughly. Distribute into sterile tubes or flasks. Use: For the cultivation and maintenance of Synechococcus species. For the isolatio...

Ngày tải lên: 03/07/2014, 18:20

... 45°–50°C. Aseptically add 100.0mL of sterile asparagine solution, 100.0mL of ster- ile methionine solution, 100.0mL of sterile sorbose solution, 10.0mL of sterile yeast extract solution, 10.0mL of sterile sodium ... Nitrofurantoin Solution: Composition per 10.0mL: Sodium nitrofurantoin 0.01g Preparation of Sodium Nitrofurantoin Solution: Add sodium nitrofurantoin to distilled/dei...

Ngày tải lên: 03/07/2014, 18:20

... isolation of various bacteria; with the addition of blood, for the cultivation of fastidious bacteria. For the cul- tivation of Bordetella pertussis for vaccine production and the mainte- nance of stock ... isolation of various bacteria; with the addition of blood, for the cultivation of fastidious bacteria. For the cul- tivation of Bordetella pertussis for vaccine productio...

Ngày tải lên: 03/07/2014, 18:20

... 7.0mL into tubes that contain meat particles (1 part meat parti- cles to 5 parts fluid). Autoclave for 30 min at 15 psi pressure–121°C. Use: For the cultivation of various anaerobes. Chopped Meat ... 7.0mL into tubes that contain meat particles (1 part meat particles to 5 parts fluid). Autoclave for 30 min at 15 psi pressure–121°C. Use: For the cultivation of numerous anaerobes, inclu...

Ngày tải lên: 03/07/2014, 18:20

... pressure–121°C. Preparation of Medium: To each tube containing 8.9mL of sterile solution A, add (using a syringe) 1.0mL of sterile solution B, 0.1mL of sterile solution C, and 0.01mL of sterile solution ... anaerobically add 100.0mL of sterile phosphate solution, 100.0mL of sterile glucose so- lution, 100.0mL of sterile NaHCO 3 solution, 10.0mL of sterile L- cysteine·HCl so...

Ngày tải lên: 03/07/2014, 18:20