Handbook of Microbiological Media, Fourth Edition part 200 pot

... llaloferax sulfurifontis, 793 llaloferax suifurifontis Medium 793 llaloferax volcanii, 787, 793, 794, 1846 llaloferax volcanii Low-Salt Medium, 793 llaloferax volcanii Medium, 793 llaloferax ... llaloferax mediterranei. 785,787,793,1346, 1420 llaloferax mediterranei Medium, 792 llaloferax mediterranei Minimal Medium I, 793 llaloferax mediterranei Minimal Medium 11. 793 llalofer...

Ngày tải lên: 03/07/2014, 18:21

... 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add 200. 0mL of sterile vitamin solution, 15.0mL of sterile glucose solution, and 1.0mL of sterile trace elments solution. Mix thoroughly. Aseptically ... Acid: Filter sterilize 40.0mL of acetic acid using a teflon filter. Preparation of Ethanol: Filter sterilize 30.0mL of ethanol using a teflon filter. Preparation of Mediu...

Ngày tải lên: 03/07/2014, 18:20

... of Acid Fuchsin Solution: Add Acid Fuchsin to 50.0mL of 5% aqueous dioxan. Mix well to dissolve. Caution: Acid Fuchsin is a potential carcinogen and care must be tak- en to avoid inhalation of ... for 15 min at 15 psi pressure–121°C. Aseptically add 0.6mL of sterile raffinose solution, 0.2mL of sterile Na 2 CO 3 solution, and 0.2mL of sterile CoCl 2 ·6H 2 O solution to each tube....

Ngày tải lên: 03/07/2014, 18:20

... elements solution 20.0mL Preparation of Base Solution: Sparge 2.0L of distilled/deionized water with 100% N 2 to remove O 2 . Add components to 2000 .0mL of O 2 -free distilled/deionized water. ... elements solution 20.0mL Preparation of Base Solution: Sparge 2.0L of distilled/deionized water with 100% N 2 to remove O 2 . Add components to 2000 .0mL of O 2 - free distilled/dei...

Ngày tải lên: 03/07/2014, 18:20

... 300.0mg Thiamine-HCl·2H 2 O 200. 0mg Nicotinic acid 200. 0mg Vitamin B 12 100.0mg Calcium pantothenate 100.0mg p-Aminobenzoic acid 80.0mg D(+)-Biotin 20.0mg Preparation of Seven Vitamin Solution: ... to 45°–50°C. Preparation of Medium: To 1.0L of sterile Eagle’s basal medium, aseptically add 1.0L of sterile, cooled agarose solution and 100.0mL of fetal calf serum. Mix thoroughly...

Ngày tải lên: 03/07/2014, 18:20

... 100.0mL of sterile vitamin B 12 solution. Mix thoroughly. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation and maintenance of Synechococcus species. For the isolation of ... 100.0mL of sterile vitamin B 12 solution. Mix thoroughly. Distribute into sterile tubes or flasks. Use: For the cultivation and maintenance of Synechococcus species. For the isolatio...

Ngày tải lên: 03/07/2014, 18:20

... 45°–50°C. Aseptically add 100.0mL of sterile asparagine solution, 100.0mL of ster- ile methionine solution, 100.0mL of sterile sorbose solution, 10.0mL of sterile yeast extract solution, 10.0mL of sterile sodium ... Nitrofurantoin Solution: Composition per 10.0mL: Sodium nitrofurantoin 0.01g Preparation of Sodium Nitrofurantoin Solution: Add sodium nitrofurantoin to distilled/dei...

Ngày tải lên: 03/07/2014, 18:20

... cultivation of Brevibacterium linens. Cherry Agar, CBS Formula Composition per liter: Pulp of sour stone cherries 200. 0g Agar 20.0g pH 3.8 ± 0.2 at 25°C Preparation of Medium: Add 1.0L of distilled/deionized ... isolation of various bacteria; with the addition of blood, for the cultivation of fastidious bacteria. For the cul- tivation of Bordetella pertussis for vaccine pro...

Ngày tải lên: 03/07/2014, 18:20

... 7.0mL into tubes that contain meat particles (1 part meat parti- cles to 5 parts fluid). Autoclave for 30 min at 15 psi pressure–121°C. Use: For the cultivation of various anaerobes. Chopped Meat ... 7.0mL into tubes that contain meat particles (1 part meat particles to 5 parts fluid). Autoclave for 30 min at 15 psi pressure–121°C. Use: For the cultivation of numerous anaerobes, inclu...

Ngày tải lên: 03/07/2014, 18:20

... pressure–121°C. Preparation of Medium: To each tube containing 8.9mL of sterile solution A, add (using a syringe) 1.0mL of sterile solution B, 0.1mL of sterile solution C, and 0.01mL of sterile solution ... anaerobically add 100.0mL of sterile phosphate solution, 100.0mL of sterile glucose so- lution, 100.0mL of sterile NaHCO 3 solution, 10.0mL of sterile L- cysteine·HCl so...

Ngày tải lên: 03/07/2014, 18:20