Handbook of Microbiological Media, Fourth Edition part 174 pot
Handbook of Microbiological Media, Fourth Edition part 174 pot
... 50.0mL of sterile solution D, 20.0mL of sterile solution
E, 10.0mL of sterile solution F, and 10.0mL of sterile solution G in
that order. Mix thoroughly.
Use: For the cultivation and maintenance of ... anaerobically add
20.0mL of sterile NaHCO
3
solution, 10.0mL of sterile CaCl
2
·2H
2
O so-
lution, 10.0mL of sterile sodium acetate solution, 10.0mL of sterile vi-
tamin solu...
Handbook of Microbiological Media, Fourth Edition part 4 potx
... Acid: Filter sterilize 40.0mL of acetic acid
using a teflon filter.
Preparation of Ethanol: Filter sterilize 30.0mL of ethanol using a
teflon filter.
Preparation of Medium: Add components, except ... anaerobically add 0.1mL of sterile
glucose, 0.1mL of sterile
L-cysteine·HCl·H
2
O solution, and 0.1mL of
sterile Na
2
S·9H
2
O solution to each tube. Mix thoroughly.
Use: For th...
Handbook of Microbiological Media, Fourth Edition part 7 potx
... of Acid Fuchsin Solution: Add Acid Fuchsin to
50.0mL of 5% aqueous dioxan. Mix well to dissolve.
Caution: Acid Fuchsin is a potential carcinogen and care must be tak-
en to avoid inhalation of ... for 15 min at 15 psi
pressure–121°C. Aseptically add 0.6mL of sterile raffinose solution,
0.2mL of sterile Na
2
CO
3
solution, and 0.2mL of sterile CoCl
2
·6H
2
O
solution to each tube....
Handbook of Microbiological Media, Fourth Edition part 9 pot
... of Medium: Aseptically combine 900.0mL of solu-
tion A with 100.0mL of solution B. Mix thoroughly. Pour into sterile
Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of ... elements solution 20.0mL
Preparation of Base Solution: Sparge 2.0L of distilled/deionized
water with 100% N
2
to remove O
2
. Add components to 2000.0mL of O
2
-
free distilled/deioni...
Handbook of Microbiological Media, Fourth Edition part 16 pot
... to 45°–50°C.
Preparation of Medium: To 1.0L of sterile Eagle’s basal medium,
aseptically add 1.0L of sterile, cooled agarose solution and 100.0mL of
fetal calf serum. Mix thoroughly. Pour into ... per 100.0mL:
Tris[hydroxymethyl]aminomethane buffer 25.0g
Preparation of Tris Buffer Solution: Add 25.0g of tris to 65.0mL of
distilled/deionized water. Titrate to pH 7.6–7.7 with con...
Handbook of Microbiological Media, Fourth Edition part 22 pot
... 100.0mL of sterile vitamin B
12
solution.
Mix thoroughly. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Synechococcus species.
For the isolation of ... 100.0mL of sterile vitamin B
12
solution.
Mix thoroughly. Distribute into sterile tubes or flasks.
Use: For the cultivation and maintenance of Synechococcus species.
For the isolatio...
Handbook of Microbiological Media, Fourth Edition part 23 pot
... 45°–50°C.
Aseptically add 100.0mL of sterile asparagine solution, 100.0mL of ster-
ile methionine solution, 100.0mL of sterile sorbose solution, 10.0mL of
sterile yeast extract solution, 10.0mL of sterile sodium ... Nitrofurantoin Solution:
Composition
per 10.0mL:
Sodium nitrofurantoin 0.01g
Preparation of Sodium Nitrofurantoin Solution: Add sodium
nitrofurantoin to distilled/dei...
Handbook of Microbiological Media, Fourth Edition part 36 pot
... isolation of various bacteria; with the
addition of blood, for the cultivation of fastidious bacteria. For the cul-
tivation of Bordetella pertussis for vaccine production and the mainte-
nance of stock ... isolation of various bacteria; with the
addition of blood, for the cultivation of fastidious bacteria. For the cul-
tivation of Bordetella pertussis for vaccine productio...
Handbook of Microbiological Media, Fourth Edition part 38 potx
... 7.0mL into tubes that contain meat particles (1 part meat parti-
cles to 5 parts fluid). Autoclave for 30 min at 15 psi pressure–121°C.
Use: For the cultivation of various anaerobes.
Chopped Meat ... 7.0mL into
tubes that contain meat particles (1 part meat particles to 5 parts fluid).
Autoclave for 30 min at 15 psi pressure–121°C.
Use: For the cultivation of numerous anaerobes, inclu...
Handbook of Microbiological Media, Fourth Edition part 43 pot
... pressure–121°C.
Preparation of Medium: To each tube containing 8.9mL of sterile
solution A, add (using a syringe) 1.0mL of sterile solution B, 0.1mL of
sterile solution C, and 0.01mL of sterile solution ... anaerobically add
100.0mL of sterile phosphate solution, 100.0mL of sterile glucose so-
lution, 100.0mL of sterile NaHCO
3
solution, 10.0mL of sterile L-
cysteine·HCl so...
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