Handbook of Microbiological Media, Fourth Edition part 166 doc

... For the differentiation of bacteria based on their ability to produce glucan. Production of glucan is indicated when the broth is partially or completely gelled—typical of Streptococcus sanguis—or ... on the bottom and walls of the tube—typi- cal of Streptococcus mutans. An increase in the viscosity indicates the production of slime (unknown polysaccharide)—typical of Streptococ...

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... 700.0mL of solu- tion A, 300.0mL of solution B, and 20.0mL of solution C. Aseptically adjust pH to 1.9–2.4 Use: For the cultivation of Sulfobacillus thermosulfidooxidans. Sulfolobus acidocaldarius ... freshly. Preparation of Medium: To 850.0mL of cooled, sterile solution A, aseptically and anaerobically add in the following order: 1.0mL of ster- ile solution B, 100.0mL of ster...

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... 11 Medium. 1664 SulfuraspiriUum Medium, 1663 SulfurospiriUum MV Medium. 1665 SulfurospiriUum species, 1043, 1666 Super HiVeg Broth. 1666 Super MMB Medium, 1666 Supcrbrotlu 1666 Supplement ... panilvinella. 1663 Sulfurimonas paralvinella Medium, 1662 Sulfurivirga caUiicttralii. 1198 Sulfuraspirillum arsenophilum, 1665 SulfurospiriUum barnesii. 1665 SulfurospiriUum del...

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... 10.0mL of CaCl 2 solution, 10.0mL of MgSO 4 ·7H 2 O solution, 10.0mL of NaNO 3 solution, 10.0mL of K 2 HPO 4 solution, 10.0mL of KH 2 PO 4 solution, and 10.0mL of NaCl solution to distilled/deionized water ... 1.0g Preparation of FeCl 3 Solution: Add FeCl 3 to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Preparation of Bristol's Solution: A...

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... pres- sure–121°C. Preparation of Medium: To 916.0mL of sterile solution A add 70.0mL of sterile solution B, 10.0mL of sterile solution C, and 10.0mL of sterile solution D. Mix thoroughly. Use: For the cultivation of anaerobic ... cultivation of a variety of anaerobic microorganisms, especially Clostridium species. Anaerobic Agar Composition per liter: Pancreatic digest of c...

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... to avoid inhalation of the powdered dye and contact with the skin. Use: For the determination of carbohydrate fermentation reactions of microorganisms, particularly members of the Enterobacteriaceae. ... to avoid inhalation of the powdered dye and contact with the skin. Use: For the determination of carbohydrate fermentation reactions of microorganisms, particularly members of...

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... determination of phenol coefficients of disinfectant prod- ucts containing cationic surface-active materials. Use according to Official Methods of Analysis of the Association of Official Analytical Chemists ... inoculation of cultures, add 0.25mL of sterile Na 2 S·9H 2 O solution to each tube containing 9.75mL of sterile basal medium. Use: For the cultivation and maintenance...

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... and anaerobically add 50.0mL of sterile cellobiose solution, 10.0mL of sterile Wolfe’s mineral solutionn, 10.0mL of sterile Wolfe’s vitamin so- lution, and 10.0mL of sterile Na 2 S·9H 2 O solution. ... Francis Group, LLC 166 Azospirillum Medium with 0.17% Agar Preparation of Bromthymol Blue Solution: Add Bromthymol Blue to 100.0mL of 0.2N KOH. Mix thoroughly. Preparation of M...

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... Aseptically add 50.0mL of sterile blood and 1.5mL of sterile water-lysed blood solution (one part blood to three parts water). Water-lysed blood may be omitted if sterile blood is partially lysed due ... Aseptically add 50.0mL of sterile blood and 1.5mL of sterile water-lysed blood solution (one part blood to three parts water). Water-lysed blood may be omitted if sterile blood is...

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... pressure–121°C. Add 0.25mL of sterile NaHCO 3 solution and 0.25mL of sterile sodium thioglycolate solu- tion for each 10.0mL of sterile basal medium. Use: For the cultivation and maintenance of Clostridium ... plate culture of other uri- nary organisms, while preventing the swarming of Proteus spp. One chromogen, X-Gluc, is targeted towards β-glucosidase, and allows the specific...

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