Handbook of Microbiological Media, Fourth Edition part 150 potx
Handbook of Microbiological Media, Fourth Edition part 150 potx
... refrigerator. Much of the particulate matter settles out.
Use the supernatant fluid.
Preparation of Medium: Combine 45.0mL of mineral solution I,
45.0mL of mineral solution II, 0.3mL of solution III, ... fluid 120.0mL
Preparation of Rumen Fluid: Filter rumen contents, obtained
from a cow on an alfalfa-hay concentrate ration, through two layers of
cheesecloth to remove larger par...
Handbook of Microbiological Media, Fourth Edition part 4 potx
... Acid: Filter sterilize 40.0mL of acetic acid
using a teflon filter.
Preparation of Ethanol: Filter sterilize 30.0mL of ethanol using a
teflon filter.
Preparation of Medium: Add components, except ... anaerobically add 0.1mL of sterile
glucose, 0.1mL of sterile
L-cysteine·HCl·H
2
O solution, and 0.1mL of
sterile Na
2
S·9H
2
O solution to each tube. Mix thoroughly.
Use: For th...
Handbook of Microbiological Media, Fourth Edition part 7 potx
... for 15 min at 15 psi
pressure–121°C. Aseptically add 0.6mL of sterile raffinose solution,
0.2mL of sterile Na
2
CO
3
solution, and 0.2mL of sterile CoCl
2
·6H
2
O
solution to each tube. Mix thoroughly. ... of Acid Fuchsin Solution: Add Acid Fuchsin to
50.0mL of 5% aqueous dioxan. Mix well to dissolve.
Caution: Acid Fuchsin is a potential carcinogen and care must be tak-
en to avoid...
Handbook of Microbiological Media, Fourth Edition part 38 potx
... 7.0mL into tubes that contain meat particles (1 part meat parti-
cles to 5 parts fluid). Autoclave for 30 min at 15 psi pressure–121°C.
Use: For the cultivation of various anaerobes.
Chopped Meat ... 7.0mL into
tubes that contain meat particles (1 part meat particles to 5 parts fluid).
Autoclave for 30 min at 15 psi pressure–121°C.
Use: For the cultivation of numerous anaerobes, inclu...
Handbook of Microbiological Media, Fourth Edition part 48 potx
... of Lactobacillus leichmannii suspension. Mix thor-
oughly. Pour into sterile 150mm Petri dishes in 50.0mL volumes.
Allow agar surface to dry before using.
Use: For the microbiological assay of ... Two
drops of sterile rabbit serum added per tube prior to solidification en-
hances the recovery of Corynebacterium diphtheriae.
Use: For the cultivation and maintenance of a variety of...
Handbook of Microbiological Media, Fourth Edition part 69 potx
...
Preparation of Medium: To 916.0mL of sterile solution A, add
70.0mL of sterile solution B, 10.0mL of sterile solution C, 10.0mL of
sterile solution D, and 10.0mL of sterile solution E. Mix thoroughly.
Anaerobically ... concentrated 15.0mL
Preparation of Trout Tissue Extract Solution: Add 1.0L of dis-
tilled/deionized water to brook trout and blend for 20–30 min. Add 1.0g
of...
Handbook of Microbiological Media, Fourth Edition part 92 potx
... add 100.0mL of sterile glucose solution,
10.0mL of sterile NaHCO
3
solution, 10.0mL of sterile CaCl
2
·2H
2
O so-
lution, 10.0mL of sterile
L-cysteine·HCl·H
2
O solution, 10.0mL of ster-
ile ... For the cultivation of pathogens in cases of bacteremia. The
medium is inoculated with blood from a patient. Approximately 10-
15mL of blood normally is inoculated into 50mL of med...
Handbook of Microbiological Media, Fourth Edition part 95 potx
... Agar
Preparation of Rabbit Blood Solution: Add 5.0mL of whole
rabbit blood to 5.0mL of sterile distilled/deionized water. Freeze and
thaw twice to lyse blood cells.
Preparation of Medium: Add components, ... of Escherichia coli.
LB Medium with 50mg of Kanamycin
Composition per liter:
NaCl 10.0g
Pancreatic digest of casein 10.0g
Yeast extract 5.0g
Kanamycin 0.05g
pH 7.0 ± 0.2 at...
Handbook of Microbiological Media, Fourth Edition part 96 potx
... 1.0mg
Preparation of Synthetic Seawater: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add 10.0g of pancreatic digest of casein to
1.0L of synthetic ... screening of blood specimens from suspected cases of
bacteremia.
Listeria Enrichment Broth
Composition per liter:
Pancreatic digest of casein 17.0g
Yeast extrac...
Handbook of Microbiological Media, Fourth Edition part 100 potx
... aseptically dilute 200.0mL of 5× stock solution
with 789.0mL of sterile distilled/deionized water. Aseptically add
10.0mL of sterile carbohydrate solution and 1.0mL of sterile
MgSO
4
·7H
2
O solution. ... anaerobically add
20.0mL of Wolfe’s vitamin solution, 10.0mL of sterile NaHCO
3
solu-
tion, 10.0mL of sterile Na
2
S·9H
2
O solution, 1.0mL Na
2
SeO
3
/Na
2
WO
4
solution, 1.0...
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