Handbook of Microbiological Media, Fourth Edition part 147 potx

... add 50.0mL of sterile glucose solu- tion, 34.0mL of sterile sodium citrate solution, 10.0mL of sterile ferric am- monium sulfate solution, 10.0mL of sterile KH 2 PO 4 solution, 10.0mL of sterile ... to each tube. Bring volume of each tube to 10.0mL with distilled/deionized water. Auto- clave for 15 min at 15 psi pressure–121°C. Use: For the microbiological assay of pyridoxine...

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... Acid: Filter sterilize 40.0mL of acetic acid using a teflon filter. Preparation of Ethanol: Filter sterilize 30.0mL of ethanol using a teflon filter. Preparation of Medium: Add components, except ... anaerobically add 0.1mL of sterile glucose, 0.1mL of sterile L-cysteine·HCl·H 2 O solution, and 0.1mL of sterile Na 2 S·9H 2 O solution to each tube. Mix thoroughly. Use: For th...

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... for 15 min at 15 psi pressure–121°C. Aseptically add 0.6mL of sterile raffinose solution, 0.2mL of sterile Na 2 CO 3 solution, and 0.2mL of sterile CoCl 2 ·6H 2 O solution to each tube. Mix thoroughly. ... of Acid Fuchsin Solution: Add Acid Fuchsin to 50.0mL of 5% aqueous dioxan. Mix well to dissolve. Caution: Acid Fuchsin is a potential carcinogen and care must be tak- en to avoid...

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... 7.0mL into tubes that contain meat particles (1 part meat parti- cles to 5 parts fluid). Autoclave for 30 min at 15 psi pressure–121°C. Use: For the cultivation of various anaerobes. Chopped Meat ... 7.0mL into tubes that contain meat particles (1 part meat particles to 5 parts fluid). Autoclave for 30 min at 15 psi pressure–121°C. Use: For the cultivation of numerous anaerobes, inclu...

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... Two drops of sterile rabbit serum added per tube prior to solidification en- hances the recovery of Corynebacterium diphtheriae. Use: For the cultivation and maintenance of a variety of fastidious microorganisms, ... cultivation and maintenance of a variety of fastidious microorganisms. For the detection of bacterial motility. Used, with added specific carbohydrate, for fermenta...

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... Preparation of Medium: To 916.0mL of sterile solution A, add 70.0mL of sterile solution B, 10.0mL of sterile solution C, 10.0mL of sterile solution D, and 10.0mL of sterile solution E. Mix thoroughly. Anaerobically ... concentrated 15.0mL Preparation of Trout Tissue Extract Solution: Add 1.0L of dis- tilled/deionized water to brook trout and blend for 20–30 min. Add 1.0g of...

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... add 100.0mL of sterile glucose solution, 10.0mL of sterile NaHCO 3 solution, 10.0mL of sterile CaCl 2 ·2H 2 O so- lution, 10.0mL of sterile L-cysteine·HCl·H 2 O solution, 10.0mL of ster- ile ... For the cultivation of pathogens in cases of bacteremia. The medium is inoculated with blood from a patient. Approximately 10- 15mL of blood normally is inoculated into 50mL of med...

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... Agar Preparation of Rabbit Blood Solution: Add 5.0mL of whole rabbit blood to 5.0mL of sterile distilled/deionized water. Freeze and thaw twice to lyse blood cells. Preparation of Medium: Add components, ... of Escherichia coli. LB Medium with 50mg of Kanamycin Composition per liter: NaCl 10.0g Pancreatic digest of casein 10.0g Yeast extract 5.0g Kanamycin 0.05g pH 7.0 ± 0.2 at...

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... 1.0mg Preparation of Synthetic Seawater: Add components to dis- tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Preparation of Medium: Add 10.0g of pancreatic digest of casein to 1.0L of synthetic ... screening of blood specimens from suspected cases of bacteremia. Listeria Enrichment Broth Composition per liter: Pancreatic digest of casein 17.0g Yeast extrac...

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... aseptically dilute 200.0mL of 5× stock solution with 789.0mL of sterile distilled/deionized water. Aseptically add 10.0mL of sterile carbohydrate solution and 1.0mL of sterile MgSO 4 ·7H 2 O solution. ... anaerobically add 20.0mL of Wolfe’s vitamin solution, 10.0mL of sterile NaHCO 3 solu- tion, 10.0mL of sterile Na 2 S·9H 2 O solution, 1.0mL Na 2 SeO 3 /Na 2 WO 4 solution, 1.0...

Ngày tải lên: 03/07/2014, 18:20