Handbook of Microbiological Media, Fourth Edition part 129 pdf
... 4.0mg Preparation of Page’s Amoeba Saline: Add components to dis- tilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Preparation of Medium: Add the glucose and ox liver digest to 1.0L of Page’s ... Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add 100.0mL of sterile solution A, 3.0mL of sterile solution B, and 1.0mL of sterile trace elements so- lutio...
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... dilution assay of pen- icillins and other antibiotics. Used in the turbidimetric assay of penicillin and tetracycline with Staphylococcus aureus. For the cultivation and main- tenance of Bacillus ... digest of gelatin 6.0g © 2010 by Taylor and Francis Group, LLC Antibiotic HiVeg Assay Medium No. 39 129 Source: This medium is available as a premixed powder from Hi- Media. Preparation...
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... Aseptically add 0.05g of chloramphenicol. Mix thoroughly. Pour into sterile Petri dishes. Use: For the isolation and presumptive identification of Cryptococcus neoformans. Cryptococcus neoformans appears ... water and bring volume to 200.0mL. Mix thoroughly. Preparation of Medium: To 400.0mL of solution A, add 400.0mL of solution B and 200.0mL of solution C. Adjust pH to 5.0. Add...
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Handbook of Microbiological Media, Fourth Edition part 31 pdf
... inhalation. Preparation of Medium: To 950.0mL of cooled sterile basal medi- um, aseptically add 50.0mL of lysed (fresh, frozen, and thawed) horse blood, 4.0mL of sterile FBP solution, 8.0mL of sterile cefoperazone ... inhalation. Preparation of Medium: To 950.0mL of cooled sterile basal medi- um, aseptically add 50.0mL of lysed (fresh, frozen, and thawed) horse blood, 4.0mL o...
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Handbook of Microbiological Media, Fourth Edition part 32 pdf
... to 25°C. Add 0.5mL of sterile carbohydrate solution to each tube. Use: For the determination of carbohydrate fermentation reactions of microorganisms, particularly members of the Enterobacteriaceae. ... to 25°C. Add 0.5mL of sterile carbohydrate solution to each tube. Use: For the determination of carbohydrate fermentation reactions of microorganisms, particularly members of t...
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Handbook of Microbiological Media, Fourth Edition part 33 pdf
... Aseptically add 50.0mL of sterile blood and 1.5mL of sterile water-lysed blood solution (one part blood to three parts water). Water-lysed blood may be omitted if sterile blood is partially lysed due ... to 50°C. Aseptically add 50.0mL of sterile rabbit blood and 1.5mL of sterile water-lysed blood solution. Water-lysed blood may be omitted if sterile blood is partially lysed due to...
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Handbook of Microbiological Media, Fourth Edition part 37 pdf
... 0.2mL of sterile solution A, 0.2mL of sterile solution B, and 0.1mL of sterile solution C for each 10.0mL of medium. Mix thoroughly. Use immediately. Use: For the cultivation and maintenance of ... intensity of 300–500 lux. For heavy cell suspension supplement periodically with sterile yeast extract solution to yield a final concen- tration of 0.1%. Use: For the growth and maint...
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Handbook of Microbiological Media, Fourth Edition part 64 pdf
... remove a sufficient amount of medium to permit the addition of feeding medium. Add 1.0mL of feeding solution per each 100.0mL of medium. Use: For the isolation and cultivation of Ectothiorhodospira ... to 25°C. Aseptically add 10.0mL of ster- ile MgCl 2 ·6H 2 O solution, 6.0mL of sterile Na 2 S·9H 2 O solution, 5.0mL of sterile CaCl 2 ·2H 2 O solution, and 4.0mL of sterile N...
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Handbook of Microbiological Media, Fourth Edition part 68 pdf
... 1.0g Preparation of Rosolic Acid Solution: Add rosolic acid to 0.2N NaOH and bring volume to 100.0L. Mix thoroughly. Preparation of Medium: Add 10.0mL of rosolic acid solution to 950.0mL of distilled/deionized ... 1.0g Preparation of Rosolic Acid Solution: Add rosolic acid to 0.2N NaOH and bring volume to 100.0L. Mix thoroughly. Preparation of Medium: Add 10.0mL of rosolic aci...
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Handbook of Microbiological Media, Fourth Edition part 86 pdf
... rubber stoppers under a gas phase of 80% H 2 + 20% CO 2 (300 kPa). Use: For the cultivation of Persephonella hydrogeniphila and Hydro- genivirga caldilitoris. Hofer’s Alkaline Medium Composition ... separate flask, add potato starch to 50.0mL of distilled/deionized water. Add the starch solution to 450.0mL of boil- ing distilled/deionized water. Add 10.0mL of solution 8 and the ag...
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