Handbook of Microbiological Media, Fourth Edition part 128 pps
Handbook of Microbiological Media, Fourth Edition part 128 pps
... Hydrolysate of Lactalbumin:
Composition
per 100.0mL:
Enzymatic hydrolysate of lactalbumin 5.0g
Preparation of Enzymatic Hydrolysate of Lactalbumin: Add
enzymatic hydrolysate of lactalbumin to 100.0mL of ... hydrolysate of lactalbumin 5.0g
Phosphate buffered saline, 1X, pH7.0 100.0mL
Preparation of Enzymatic Hydrolysate of Lactalbumin: Add
enzymatic hydrolysate of lactalbu...
Handbook of Microbiological Media, Fourth Edition part 2 pps
... examination of water and food.
Sources of Media
The Handbook of Microbiological Media includes the media produced
by major suppliers of dehydrated media, including Oxoid Unipath,
HiMedia, and ... to
cultivate microorganisms.
Uses of Media
The Handbook of Microbiological Media contains all the media used to
cultivate bacteria, archaea, fungi, and protists of the Americ...
Handbook of Microbiological Media, Fourth Edition part 3 pps
... tubes.
Use: For the maintenance of cultures of Escherichia coli and Staphy-
lococcus aureus. For the detection of antibacterial activity of fabrics.
Test cultures of Escherichia coli or Staphylococcus ... Prior to inoculation of cultures, aseptically and
anaerobically add 0.1mL of sterile reducing agent solution and 0.5mL
of sterile glucose solution to each tube containing 9.4...
Handbook of Microbiological Media, Fourth Edition part 6 ppsx
... add 5.0mL of sterile ferric citrate solution
and 1.0mL of sterile trace elements solution. Mix thoroughly. Asepti-
cally distribute into sterile tubes or flasks.
Use: For the cultivation of Rhodopseudomonas ... inoculation, aseptically and anaerobically
add 0.25–0.50mL of neutralized sulfide solution.
Use: For the cultivation and maintenance of members of the family
Rhodospirillac...
Handbook of Microbiological Media, Fourth Edition part 12 pps
... anaerobi-
cally add 20.0mL of sterile glucose solution. Aseptically and
anaerobically distribute into sterile tubes or bottles.
Use: For the cultivation of Anaerofilum agile and Anaerofilum pentos-
ovorans. ... dishes or leave in tubes.
Use: For the cultivation of a variety of anaerobic microorganisms.
With added blood for the detection of hemolytic activity of clostridia,
strepto...
Handbook of Microbiological Media, Fourth Edition part 21 ppsx
... 100.0mL:
FeSO
4
·7H
2
O 0.2g
Preparation of FeSO
4
·7H
2
O Solution: Dissolve FeSO
4
·7H
2
O in
100.0mL of distilled/deionized water. Add three drops of concentrated
HCl. Mix thoroughly.
Vitamin ... isolation of Gram-negative enteric bacilli
from clinical and nonclinical specimens. For the isolation and identifi-
cation of microorganisms from fecal specimens. For the isolation of...
Handbook of Microbiological Media, Fourth Edition part 24 ppsx
... Aseptically add 150.0mL of sterile
liver extract, 50.0mL of sterile horse blood, 10.0mL of sterile
L-
cysteine·HCl solution, 10.0 mL of sterile solution A, and 5.0mL of ster-
ile solution B. ... final concentration of
7%. Pour into sterile Petri dishes.
Use: For the isolation, cultivation, and detection of hemolytic activity of
streptococci, pneumococci, and other particularl...
Handbook of Microbiological Media, Fourth Edition part 27 pps
... the detection of E. coli and coliforms. Bile and Brilliant
Green extensively inhibit the growth of accompanying flora, in partic-
ular Gram-positive microorganisms. The presence of E. coli results ... tube to yield a final
carbohydrate concentration of 5%.
Use: For the differentiation of a variety of microorganisms based on
their fermentation of specific carbohydrates. Bacteria...
Handbook of Microbiological Media, Fourth Edition part 28 ppsx
... aseptically add 27.0mL of
sterile lactose solution, 10.0mL of sterile Bromthymol Blue solution,
2.0mL of sterile sodium thiosulfate solution, 1.2mL of sterile glucose so-
lution, and 1.0mL of sterile maranil ... 45°–50°C. Aseptically add 10.0mL of
sterile horse serum, 1.0mL of sterile ampicillin solution, 0.25mL of ster-
ile urea solution, 0.1mL of sterile nystatin solution,...
Handbook of Microbiological Media, Fourth Edition part 29 ppsx
... 25.0g
Preparation of Glucose Solution: Add 25.0g of glucose to 50.0mL
of distilled/deionized water. Mix thoroughly and gently heat to dissolve.
Filter sterilize.
Preparation of Medium: Add components, ... medium. Aseptically add 72.0mL of sterile rabbit
serum.
Use: For the cultivation of a wide variety of microorganisms in a
chemically defined medium. For the cultivation of Bo...
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