Handbook of Microbiological Media, Fourth Edition part 125 pptx
Handbook of Microbiological Media, Fourth Edition part 125 pptx
... add20.0mL of sterile glucose solution and 10.0mL of sterile yeast extractsolution. Mix thoroughly. Aseptically distribute into sterile tubes orflasks.Use: For the cultivation of Acidiphilium ... in tubes.Use: For the cultivation of Runella slithyformis.MS 1 AgarComposition per liter:Agar 15.0gSeawater 1.0LPreparation of Medium: Add agar to 1.0L of natural seawater. Mixthoroughly. ... 15 min at 15 psi pressure–121°C. Use: For the cultivation of Lactobacillus kefiranofaciens.MRS, ModifiedSee: Lactobacillus Heteroferm Screen BrothMRS Medium with L-CysteineComposition per...
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Handbook of Microbiological Media, Fourth Edition part 1 pptx
... the numbers of particular species of microorganisms. Most often, mixtures of growth factors are used in microbiological media. Acid hydrolysates of casein commonly are usedas sources of amino acids. ... digest of soybean meal. It has a high vitamin and a highcarbohydrate content. Polypeptone™ PeptoneA mixture of peptones composed of equal parts of pancreaticdigest of casein and peptic digest of ... high levels of amino acids. It contains total nitrogen of 11.9% and NaCl of 1.4%. Liver Digest NeutralizedA papaic digest of liver that contains total nitrogen of 11.0%and NaCl of 1.6%. Mycological...
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Handbook of Microbiological Media, Fourth Edition part 17 pptx
... com-bine 500.0mL of solution 1 with 500.0mL of solution 2.Nutrient Agar:Composition per liter:Agar 15.0gPancreatic digest of gelatin 5.0gBeef extract 3.0gPreparation of Nutrient Agar: ... 0.1gPreparation of NAD Solution: Add NAD to distilled/deionizedwater and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Add 10.0g of agar to 700.0mL of PPLObroth ... 25°CPreparation of Medium: Add components to distilled/deionizedwater and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 minat 15 psi pressure–121°C. Use: For the maintenance of cultures of streptococci...
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Handbook of Microbiological Media, Fourth Edition part 20 pptx
... temperature for 20 min. Add 20.0mL of dis-tilled/deionized H2O. Filter through a layer of cheesecloth. To 90–100.0mL of filtrate add 0.8% NaCl solution to bring volume to 125. 0mL. Autoclave for 20 ... fragilis group. For the differentiation of Bacteroides speciesbased on the hydrolysis of esculin and presence of catalase. After incu-bation for 48 hr, bacteria of the Bacteroides fragilis group ... For the cultivation of a wide variety of bacteria. BAGG Broth(Buffered Azide Glucose Glycerol Broth)Composition per liter:Pancreatic digest of casein 10.0gPeptic digest of animal tissue 10.0gGlucose...
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Handbook of Microbiological Media, Fourth Edition part 35 pptx
... 45°–50°C.Preparation of Cellulose Overlay Agar: Aseptically combine350.0mL of cooled, sterile solution A and 650.0mL of cooled, sterilesolution B. Mix thoroughly. Preparation of Medium: Pour cooled, ... 0.25gNa2S·9H2O 0.25gPreparation of Alkaline Solution: Add components to 25.0mL of distilled/deionized water. Mix thoroughly. Prepare freshly.Preparation of Medium: Prepare 975.0mL of basal medium. Heatto ... 50.0mL of ster-ile cellobiose solution, 10.0mL of sterile NaHCO3 solution, and10.0mL of sterile L-cysteine·HCl solution. Mix thoroughly.Use: For the cultivation and maintenance of Cellulomonas...
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Handbook of Microbiological Media, Fourth Edition part 53 pptx
... combine870.0mL of sterile solution A with 1.0mL of sterile solution B,100.0mL of sterile solution C, 10.0mL of sterile solution D, 10.0mL of sterile solution E, and 10.0mL of sterile solution ... Medium: To 1.0L of sterile solution A, add in or-der: 1.0mL of sterile solution B, 2.0mL of sterile solution C, 50.0mL of sterile solution D, 10.0mL of sterile solution E, 1.0mL of sterile so-lution ... freshly.Preparation of Medium: To 900.0mL of cooled, sterile solution A,aseptically and anaerobically add in the following order: 1.0mL of sterilesolution B, 50.0mL of sterile solution C, 10.0mL of sterile...
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Handbook of Microbiological Media, Fourth Edition part 54 pptx
... combine870.0mL of sterile solution A with 1.0mL of sterile solution B,100.0mL of sterile solution C, 10.0mL of sterile solution D, 10.0mL of sterile solution E, and 10.0mL of sterile solution ... 5.0mgRiboflavin 5.0mgThiamine·HCl 5.0mgBiotin 5.0mgFolic acid 5.0mgVitamin B12 5.0mgHemin 5.0mgPreparation of Solution E (Vitamin Solution): Add 1,4-naphtho-quinone and hemin to 10.0mL of ... 5.0mgPyridoxine·HCl 5.0mgRiboflavin 5.0mgThioctic acid 5.0mgNaOH (0.1N solution) 5.0mLPreparation of Vitamin Solution: Add thioctic acid, 1,4-naphtho-quinone, and hemin to 5.0mL of 0.1N NaOH solution....
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Handbook of Microbiological Media, Fourth Edition part 55 pptx
... 1.0mL of sterile solution E, 1.0mL of sterile solution F, 10.0mL of sterile solution G, 1.0mL of sterile solution H, 6.6mL of sterile solutionI, 20.0mL of sterile solution J, and 10.0mL of sterile ... 10.0mL of sterile solutionD, 1.0mL of sterile solution E, 10.0mL of sterile solution F, 6.6mL of sterile solution G, 20.0mL of sterile solution H, and 10.0mL of sterilesolution I. Mix thoroughly. ... solu-tion.Preparation of Medium: To 890.0mL of cooled, sterile solution A,aseptically and anaerobically add in the following order: 1.0mL of ster-ile solution B, 50.0mL of sterile solution C, 10.0mL of sterile...
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Handbook of Microbiological Media, Fourth Edition part 57 pptx
... To 1.0L of sterile solution A, add in the fol-lowing order: 1.0mL of sterile solution B, 2.0mL of sterile solution C,50.0mL of sterile solution D, 10.0mL of sterile solution E, 1.0mL of ster-ile ... solution B,100.0mL of sterile solution C, 10.0mL of sterile solution D, 10.0mL of sterile solution E, 6.6mL of sterile solution F, 20.0mL of sterile solution G,and 10.0mL of sterile solution ... F, 10.0mL of sterile solution G, 67.0mL of sterile solution H,and 13.0mL of sterile solution I. Mix thoroughly. Final pH of mediumshould be 7.2. Prior to inoculation, add 10.0–20.0mg of sodium...
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Handbook of Microbiological Media, Fourth Edition part 58 pptx
... freshly.Preparation of Medium: To 920.0mL of cooled, sterile solution A,aseptically and anaerobically add in the following order: 1.0mL of ster-ile solution B, 50.0mL of sterile solution C, 10.0mL of sterile ... anaerobically add 10.0mL of sterileNa2S·9H2O solution and 5.0g of sterile powdered sulfur. Mix thor-oughly. Final pH of medium should be 5.5.Use: For the cultivation and maintenance of Desulfurococcus ... 15 psi pressure–121°C.Preparation of Sulfur: Add 10.0g of powdered sulfur to a flask andsterilize by steaming for 3 hr on 3 consecutive days.Preparation of Medium: Prepare and dispense medium...
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