Handbook of Microbiological Media, Fourth Edition part 125 pptx

... add 20.0mL of sterile glucose solution and 10.0mL of sterile yeast extract solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use: For the cultivation of Acidiphilium ... in tubes. Use: For the cultivation of Runella slithyformis. MS 1 Agar Composition per liter: Agar 15.0g Seawater 1.0L Preparation of Medium: Add agar to 1.0L of natural seawater. Mix t...

Ngày tải lên: 03/07/2014, 18:21

... the numbers of particular species of microorganisms. Most often, mixtures of growth factors are used in microbiological media. Acid hydrolysates of casein commonly are used as sources of amino acids. ... digest of soybean meal. It has a high vitamin and a high carbohydrate content. Polypeptone™ Peptone A mixture of peptones composed of equal parts of pancreatic digest of...

Ngày tải lên: 03/07/2014, 18:20

... com- bine 500.0mL of solution 1 with 500.0mL of solution 2. Nutrient Agar: Composition per liter: Agar 15.0g Pancreatic digest of gelatin 5.0g Beef extract 3.0g Preparation of Nutrient Agar: ... 0.1g Preparation of NAD Solution: Add NAD to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Add 10.0g of agar to 700.0mL...

Ngày tải lên: 03/07/2014, 18:20

... temperature for 20 min. Add 20.0mL of dis- tilled/deionized H 2 O. Filter through a layer of cheesecloth. To 90– 100.0mL of filtrate add 0.8% NaCl solution to bring volume to 125. 0mL. Autoclave for 20 ... fragilis group. For the differentiation of Bacteroides species based on the hydrolysis of esculin and presence of catalase. After incu- bation for 48 hr, bacteria of the Bact...

Ngày tải lên: 03/07/2014, 18:20

... 45°–50°C. Preparation of Cellulose Overlay Agar: Aseptically combine 350.0mL of cooled, sterile solution A and 650.0mL of cooled, sterile solution B. Mix thoroughly. Preparation of Medium: Pour cooled, ... 0.25g Na 2 S·9H 2 O 0.25g Preparation of Alkaline Solution: Add components to 25.0mL of distilled/deionized water. Mix thoroughly. Prepare freshly. Preparation of Medium: Pre...

Ngày tải lên: 03/07/2014, 18:20

... combine 870.0mL of sterile solution A with 1.0mL of sterile solution B, 100.0mL of sterile solution C, 10.0mL of sterile solution D, 10.0mL of sterile solution E, and 10.0mL of sterile solution ... Medium: To 1.0L of sterile solution A, add in or- der: 1.0mL of sterile solution B, 2.0mL of sterile solution C, 50.0mL of sterile solution D, 10.0mL of sterile solution E, 1....

Ngày tải lên: 03/07/2014, 18:20

... combine 870.0mL of sterile solution A with 1.0mL of sterile solution B, 100.0mL of sterile solution C, 10.0mL of sterile solution D, 10.0mL of sterile solution E, and 10.0mL of sterile solution ... 5.0mg Riboflavin 5.0mg Thiamine·HCl 5.0mg Biotin 5.0mg Folic acid 5.0mg Vitamin B 12 5.0mg Hemin 5.0mg Preparation of Solution E (Vitamin Solution): Add 1,4-naphtho- quinone and hemi...

Ngày tải lên: 03/07/2014, 18:20

... 1.0mL of sterile solution E, 1.0mL of sterile solution F, 10.0mL of sterile solution G, 1.0mL of sterile solution H, 6.6mL of sterile solution I, 20.0mL of sterile solution J, and 10.0mL of sterile ... 10.0mL of sterile solution D, 1.0mL of sterile solution E, 10.0mL of sterile solution F, 6.6mL of sterile solution G, 20.0mL of sterile solution H, and 10.0mL of steri...

Ngày tải lên: 03/07/2014, 18:20

... To 1.0L of sterile solution A, add in the fol- lowing order: 1.0mL of sterile solution B, 2.0mL of sterile solution C, 50.0mL of sterile solution D, 10.0mL of sterile solution E, 1.0mL of ster- ile ... solution B, 100.0mL of sterile solution C, 10.0mL of sterile solution D, 10.0mL of sterile solution E, 6.6mL of sterile solution F, 20.0mL of sterile solution G, and 10.0m...

Ngày tải lên: 03/07/2014, 18:20

... freshly. Preparation of Medium: To 920.0mL of cooled, sterile solution A, aseptically and anaerobically add in the following order: 1.0mL of ster- ile solution B, 50.0mL of sterile solution C, 10.0mL of sterile ... anaerobically add 10.0mL of sterile Na 2 S·9H 2 O solution and 5.0g of sterile powdered sulfur. Mix thor- oughly. Final pH of medium should be 5.5. Use: For the cul...

Ngày tải lên: 03/07/2014, 18:20