Handbook of Microbiological Media, Fourth Edition part 104 pps

... formate solution, 10.0mL of sterile cysteine solution, and 10.0mL of sterile Na 2 S·9H 2 O solution. Mix thoroughly. The final pH should be 6.5. Use: For the cultivation of Methanofollis aquaemaris ... Cool to 45°–50°C. Aseptically add 20.0mL of sterile Sarci- na lutea suspension, 20.0mL of sterile horse serum, 10.0mL of supplement solution, and 10.0mL of sterile PCAT inhibitor....

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... examination of water and food. Sources of Media The Handbook of Microbiological Media includes the media produced by major suppliers of dehydrated media, including Oxoid Unipath, HiMedia, and ... to cultivate microorganisms. Uses of Media The Handbook of Microbiological Media contains all the media used to cultivate bacteria, archaea, fungi, and protists of the Americ...

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... tubes. Use: For the maintenance of cultures of Escherichia coli and Staphy- lococcus aureus. For the detection of antibacterial activity of fabrics. Test cultures of Escherichia coli or Staphylococcus ... Prior to inoculation of cultures, aseptically and anaerobically add 0.1mL of sterile reducing agent solution and 0.5mL of sterile glucose solution to each tube containing 9.4...

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... add 5.0mL of sterile ferric citrate solution and 1.0mL of sterile trace elements solution. Mix thoroughly. Asepti- cally distribute into sterile tubes or flasks. Use: For the cultivation of Rhodopseudomonas ... inoculation, aseptically and anaerobically add 0.25–0.50mL of neutralized sulfide solution. Use: For the cultivation and maintenance of members of the family Rhodospirillac...

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... anaerobi- cally add 20.0mL of sterile glucose solution. Aseptically and anaerobically distribute into sterile tubes or bottles. Use: For the cultivation of Anaerofilum agile and Anaerofilum pentos- ovorans. ... dishes or leave in tubes. Use: For the cultivation of a variety of anaerobic microorganisms. With added blood for the detection of hemolytic activity of clostridia, strepto...

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... 100.0mL: FeSO 4 ·7H 2 O 0.2g Preparation of FeSO 4 ·7H 2 O Solution: Dissolve FeSO 4 ·7H 2 O in 100.0mL of distilled/deionized water. Add three drops of concentrated HCl. Mix thoroughly. Vitamin ... isolation of Gram-negative enteric bacilli from clinical and nonclinical specimens. For the isolation and identifi- cation of microorganisms from fecal specimens. For the isolation of...

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... Aseptically add 150.0mL of sterile liver extract, 50.0mL of sterile horse blood, 10.0mL of sterile L- cysteine·HCl solution, 10.0 mL of sterile solution A, and 5.0mL of ster- ile solution B. ... final concentration of 7%. Pour into sterile Petri dishes. Use: For the isolation, cultivation, and detection of hemolytic activity of streptococci, pneumococci, and other particularl...

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... the detection of E. coli and coliforms. Bile and Brilliant Green extensively inhibit the growth of accompanying flora, in partic- ular Gram-positive microorganisms. The presence of E. coli results ... tube to yield a final carbohydrate concentration of 5%. Use: For the differentiation of a variety of microorganisms based on their fermentation of specific carbohydrates. Bacteria...

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... aseptically add 27.0mL of sterile lactose solution, 10.0mL of sterile Bromthymol Blue solution, 2.0mL of sterile sodium thiosulfate solution, 1.2mL of sterile glucose so- lution, and 1.0mL of sterile maranil ... 45°–50°C. Aseptically add 10.0mL of sterile horse serum, 1.0mL of sterile ampicillin solution, 0.25mL of ster- ile urea solution, 0.1mL of sterile nystatin solution,...

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... 25.0g Preparation of Glucose Solution: Add 25.0g of glucose to 50.0mL of distilled/deionized water. Mix thoroughly and gently heat to dissolve. Filter sterilize. Preparation of Medium: Add components, ... medium. Aseptically add 72.0mL of sterile rabbit serum. Use: For the cultivation of a wide variety of microorganisms in a chemically defined medium. For the cultivation of Bo...

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