Handbook of Microbiological Media, Fourth Edition part 88 ppt
Handbook of Microbiological Media, Fourth Edition part 88 ppt
... Aseptically and anaerobically add 20.0mL of sterile NaHCO 3 solution, 10.0mL of sterile sodium L-tartrate solution, 10.0mL of sterile yeast extract solution, and 10.0mL of sterile Na 2 S·9H 2 O solution. ... 1.0L of sterile solution A, add 10.0mL of sterile solution B and 1.0mL of sterile vitamin solution. Mix thor- oughly. Adjust final pH to 7.2–7.4. Use: For the cultivation a...
Ngày tải lên: 03/07/2014, 18:20
Handbook of Microbiological Media, Fourth Edition part 1 pptx
... the numbers of particular species of microorganisms. Most often, mixtures of growth factors are used in microbiological media. Acid hydrolysates of casein commonly are used as sources of amino acids. ... digest of soybean meal. It has a high vitamin and a high carbohydrate content. Polypeptone™ Peptone A mixture of peptones composed of equal parts of pancreatic digest of...
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Handbook of Microbiological Media, Fourth Edition part 10 ppt
... 5.2g Preparation of A & A FeEDTA Solution: Dissolve 5.2g of KOH in 186.0mL of distilled/deionized water. Add 20.4g of disodium EDTA·2H 2 O. Add 13.7g of FeSO4·7H2O to 364.0mL of distilled/deion- ized ... pressure–121°C. Preparation of Medium: Saturate cooled solution A under 100% CO 2 at 0.05–0.1 atm pressure for 30 min with magnetic stirring. Add 860.0mL of solution B, 5...
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Handbook of Microbiological Media, Fourth Edition part 17 pptx
... pneumoniae 10 12 cells Preparation of Heat-Killed Bacterial Suspension: Inoculate a loopful of Klebsiella pneumoniae subsp. pneumoniae ATCC 2 7889 into 5.0mL of nutrient broth. Incubate at 35°C ... com- bine 500.0mL of solution 1 with 500.0mL of solution 2. Nutrient Agar: Composition per liter: Agar 15.0g Pancreatic digest of gelatin 5.0g Beef extract 3.0g Preparation of Nut...
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Handbook of Microbiological Media, Fourth Edition part 19 ppt
... 15 min at 15 psi pressure–121°C. Preparation of Medium: Aseptically combine 500.0mL of sterile solution A, 500.0mL of sterile solution C, and 1.0mL of sterile solution B. Mix thoroughly. Pour ... Preparation of Medium: Aseptically combine 1.0L of sterile solu- tion A and 1.0mL of sterile solution B. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use: For...
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Handbook of Microbiological Media, Fourth Edition part 20 pptx
... fragilis group. For the differentiation of Bacteroides species based on the hydrolysis of esculin and presence of catalase. After incu- bation for 48 hr, bacteria of the Bacteroides fragilis group ... For the cultivation of a wide variety of bacteria. BAGG Broth (Buffered Azide Glucose Glycerol Broth) Composition per liter: Pancreatic digest of casein 10.0g Peptic digest of a...
Ngày tải lên: 03/07/2014, 18:20
Handbook of Microbiological Media, Fourth Edition part 25 ppt
... liter: Agar 20.0g NaCl 5.5g Pancreatic digest of casein 5.0g Peptic digest of animal tissue 5.0g Preparation of Bordet-Gengou Agar Base: Add components to 1.0L of 1% glycerol solution. Autoclave for ... 10.0g NaCl 5.0g Pancreatic digest of casein 5.0g Peptic digest of animal tissue 5.0g Charcoal 4.0g Nicotinic acid 1.0mg Preparation of Charcoal Agar Base: Add components of cha...
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Handbook of Microbiological Media, Fourth Edition part 35 pptx
... 45°–50°C. Preparation of Cellulose Overlay Agar: Aseptically combine 350.0mL of cooled, sterile solution A and 650.0mL of cooled, sterile solution B. Mix thoroughly. Preparation of Medium: Pour cooled, ... 0.25g Na 2 S·9H 2 O 0.25g Preparation of Alkaline Solution: Add components to 25.0mL of distilled/deionized water. Mix thoroughly. Prepare freshly. Preparation of Medium: Pre...
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Handbook of Microbiological Media, Fourth Edition part 45 ppt
... from other coliform colonies which have a salmon to red color. As part of an additional confirmation of E. coli, the inclusion of tryptophan improves the indole reaction, thereby increasing detection ... For the detection of E.coli and total coliforms. The combination of suitable peptones and the buffering using MOPS allows rapid growth of coliforms and an optimal transformation...
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Handbook of Microbiological Media, Fourth Edition part 53 pptx
... combine 870.0mL of sterile solution A with 1.0mL of sterile solution B, 100.0mL of sterile solution C, 10.0mL of sterile solution D, 10.0mL of sterile solution E, and 10.0mL of sterile solution ... Medium: To 1.0L of sterile solution A, add in or- der: 1.0mL of sterile solution B, 2.0mL of sterile solution C, 50.0mL of sterile solution D, 10.0mL of sterile solution E, 1....
Ngày tải lên: 03/07/2014, 18:20