Handbook of Microbiological Media, Fourth Edition part 87 ppt

... min at 15 psi pressure–121°C. Cool to 45°–50°C. Preparation of Medium: Add 167.0mL of solution A, 167.0mL of so- lution B, and 1.0mL of resazurin solution to distilled/deionized water and bring ... aseptically and anaerobically add 0.1mL of sterile L-cysteine·HCl solution, 0.5mL of sterile NaHCO 3 solution, and 0.1mL of substrate solution per 10.0mL of medium in each tube....

Ngày tải lên: 03/07/2014, 18:20

... the numbers of particular species of microorganisms. Most often, mixtures of growth factors are used in microbiological media. Acid hydrolysates of casein commonly are used as sources of amino acids. ... digest of soybean meal. It has a high vitamin and a high carbohydrate content. Polypeptone™ Peptone A mixture of peptones composed of equal parts of pancreatic digest of...

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... 5.2g Preparation of A & A FeEDTA Solution: Dissolve 5.2g of KOH in 186.0mL of distilled/deionized water. Add 20.4g of disodium EDTA·2H 2 O. Add 13.7g of FeSO4·7H2O to 364.0mL of distilled/deion- ized ... pressure–121°C. Preparation of Medium: Saturate cooled solution A under 100% CO 2 at 0.05–0.1 atm pressure for 30 min with magnetic stirring. Add 860.0mL of solution B, 5...

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... com- bine 500.0mL of solution 1 with 500.0mL of solution 2. Nutrient Agar: Composition per liter: Agar 15.0g Pancreatic digest of gelatin 5.0g Beef extract 3.0g Preparation of Nutrient Agar: ... 0.1g Preparation of NAD Solution: Add NAD to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Add 10.0g of agar to 700.0mL...

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... 15 min at 15 psi pressure–121°C. Preparation of Medium: Aseptically combine 500.0mL of sterile solution A, 500.0mL of sterile solution C, and 1.0mL of sterile solution B. Mix thoroughly. Pour ... Preparation of Medium: Aseptically combine 1.0L of sterile solu- tion A and 1.0mL of sterile solution B. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use: For...

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... fragilis group. For the differentiation of Bacteroides species based on the hydrolysis of esculin and presence of catalase. After incu- bation for 48 hr, bacteria of the Bacteroides fragilis group ... For the cultivation of a wide variety of bacteria. BAGG Broth (Buffered Azide Glucose Glycerol Broth) Composition per liter: Pancreatic digest of casein 10.0g Peptic digest of a...

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... liter: Agar 20.0g NaCl 5.5g Pancreatic digest of casein 5.0g Peptic digest of animal tissue 5.0g Preparation of Bordet-Gengou Agar Base: Add components to 1.0L of 1% glycerol solution. Autoclave for ... 10.0g NaCl 5.0g Pancreatic digest of casein 5.0g Peptic digest of animal tissue 5.0g Charcoal 4.0g Nicotinic acid 1.0mg Preparation of Charcoal Agar Base: Add components of cha...

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... 45°–50°C. Preparation of Cellulose Overlay Agar: Aseptically combine 350.0mL of cooled, sterile solution A and 650.0mL of cooled, sterile solution B. Mix thoroughly. Preparation of Medium: Pour cooled, ... 0.25g Na 2 S·9H 2 O 0.25g Preparation of Alkaline Solution: Add components to 25.0mL of distilled/deionized water. Mix thoroughly. Prepare freshly. Preparation of Medium: Pre...

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... from other coliform colonies which have a salmon to red color. As part of an additional confirmation of E. coli, the inclusion of tryptophan improves the indole reaction, thereby increasing detection ... For the detection of E.coli and total coliforms. The combination of suitable peptones and the buffering using MOPS allows rapid growth of coliforms and an optimal transformation...

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... anaerobically combine 870 .0mL of sterile solution A with 1.0mL of sterile solution B, 100.0mL of sterile solution C, 10.0mL of sterile solution D, 10.0mL of sterile solution E, and 10.0mL of sterile solution ... Medium: To 1.0L of sterile solution A, add in or- der: 1.0mL of sterile solution B, 2.0mL of sterile solution C, 50.0mL of sterile solution D, 10.0mL of steril...

Ngày tải lên: 03/07/2014, 18:20