Handbook of Microbiological Media, Fourth Edition part 70 pptx
... 100.0mg ZnCl 2 70. 0mg Na 2 MoO 4 ·2H 2 O 36.0mg NiCl 2 ·6H 2 O 24.0mg CuCl 2 ·2H 2 O 2.0mg HCl (25% solution) 10.0mL Preparation of Trace Elements Solution SL-10: Add FeCl 2 ·4H 2 O to 10.0mL of HCl ... psi pressure–121°C. Cool to 45°–50°C. Aseptically add 10.0mL of sterile vitamin solution, 10.0mL of sterile glucose solution, and 1.0mL of sterile trace elments solution. Mix thor...
Ngày tải lên: 03/07/2014, 18:20
... the numbers of particular species of microorganisms. Most often, mixtures of growth factors are used in microbiological media. Acid hydrolysates of casein commonly are used as sources of amino acids. ... digest of soybean meal. It has a high vitamin and a high carbohydrate content. Polypeptone™ Peptone A mixture of peptones composed of equal parts of pancreatic digest of...
Ngày tải lên: 03/07/2014, 18:20
... 0.1g Preparation of NAD Solution: Add NAD to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Add 10.0g of agar to 700 .0mL of PPLO broth ... com- bine 500.0mL of solution 1 with 500.0mL of solution 2. Nutrient Agar: Composition per liter: Agar 15.0g Pancreatic digest of gelatin 5.0g Beef extract 3.0g Preparation...
Ngày tải lên: 03/07/2014, 18:20
Handbook of Microbiological Media, Fourth Edition part 20 pptx
... fragilis group. For the differentiation of Bacteroides species based on the hydrolysis of esculin and presence of catalase. After incu- bation for 48 hr, bacteria of the Bacteroides fragilis group ... For the cultivation of a wide variety of bacteria. BAGG Broth (Buffered Azide Glucose Glycerol Broth) Composition per liter: Pancreatic digest of casein 10.0g Peptic digest of a...
Ngày tải lên: 03/07/2014, 18:20
Handbook of Microbiological Media, Fourth Edition part 35 pptx
... 45°–50°C. Preparation of Cellulose Overlay Agar: Aseptically combine 350.0mL of cooled, sterile solution A and 650.0mL of cooled, sterile solution B. Mix thoroughly. Preparation of Medium: Pour cooled, ... 0.25g Na 2 S·9H 2 O 0.25g Preparation of Alkaline Solution: Add components to 25.0mL of distilled/deionized water. Mix thoroughly. Prepare freshly. Preparation of Medium: Pre...
Ngày tải lên: 03/07/2014, 18:20
Handbook of Microbiological Media, Fourth Edition part 53 pptx
... anaerobically combine 870. 0mL of sterile solution A with 1.0mL of sterile solution B, 100.0mL of sterile solution C, 10.0mL of sterile solution D, 10.0mL of sterile solution E, and 10.0mL of sterile solution ... Medium: To 1.0L of sterile solution A, add in or- der: 1.0mL of sterile solution B, 2.0mL of sterile solution C, 50.0mL of sterile solution D, 10.0mL of steril...
Ngày tải lên: 03/07/2014, 18:20
Handbook of Microbiological Media, Fourth Edition part 54 pptx
... anaerobically combine 870. 0mL of sterile solution A with 1.0mL of sterile solution B, 100.0mL of sterile solution C, 10.0mL of sterile solution D, 10.0mL of sterile solution E, and 10.0mL of sterile solution ... A: Composition per 870. 0mL: NaCl 20.0g Na 2 SO 4 1.42g KCl 0.67g NH 4 Cl 0.1g KH 2 PO 4 0.01g MgSO 4 ·7H 2 O 0.02g Resazurin 0.5mg Preparation of Solution A: Add c...
Ngày tải lên: 03/07/2014, 18:20
Handbook of Microbiological Media, Fourth Edition part 55 pptx
... 1.0mL of sterile solution E, 1.0mL of sterile solution F, 10.0mL of sterile solution G, 1.0mL of sterile solution H, 6.6mL of sterile solution I, 20.0mL of sterile solution J, and 10.0mL of sterile ... 10.0mL of sterile solution D, 1.0mL of sterile solution E, 10.0mL of sterile solution F, 6.6mL of sterile solution G, 20.0mL of sterile solution H, and 10.0mL of steri...
Ngày tải lên: 03/07/2014, 18:20
Handbook of Microbiological Media, Fourth Edition part 57 pptx
... To 1.0L of sterile solution A, add in the fol- lowing order: 1.0mL of sterile solution B, 2.0mL of sterile solution C, 50.0mL of sterile solution D, 10.0mL of sterile solution E, 1.0mL of ster- ile ... solution B, 100.0mL of sterile solution C, 10.0mL of sterile solution D, 10.0mL of sterile solution E, 6.6mL of sterile solution F, 20.0mL of sterile solution G, and 10.0m...
Ngày tải lên: 03/07/2014, 18:20
Handbook of Microbiological Media, Fourth Edition part 58 pptx
... freshly. Preparation of Medium: To 920.0mL of cooled, sterile solution A, aseptically and anaerobically add in the following order: 1.0mL of ster- ile solution B, 50.0mL of sterile solution C, 10.0mL of sterile ... anaerobically add 10.0mL of sterile Na 2 S·9H 2 O solution and 5.0g of sterile powdered sulfur. Mix thor- oughly. Final pH of medium should be 5.5. Use: For the cul...
Ngày tải lên: 03/07/2014, 18:20