Handbook of Microbiological Media, Fourth Edition part 65 ppsx
Handbook of Microbiological Media, Fourth Edition part 65 ppsx
... 14.0g Lactose 9.4g Peptones (pancreatic digest of casein 65% and yeast extract 35%) 7.5g NaCl 3.7g Pancreatic digest of casein 3.7g Peptic digest of animal tissue 3.7g K 2 HPO 4 3.3g Na 2 SO 3 ... to avoid inhalation of the powdered dye and contact with the skin. Preparation of Basic Fuchsin Solution: Add Basic Fuchsin to 10.0mL of ethanol. Mix thoroughly. Preparation of Me...
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Handbook of Microbiological Media, Fourth Edition part 6 ppsx
... add 5.0mL of sterile ferric citrate solution and 1.0mL of sterile trace elements solution. Mix thoroughly. Asepti- cally distribute into sterile tubes or flasks. Use: For the cultivation of Rhodopseudomonas ... inoculation, aseptically and anaerobically add 0.25–0.50mL of neutralized sulfide solution. Use: For the cultivation and maintenance of members of the family Rhodospirillac...
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Handbook of Microbiological Media, Fourth Edition part 21 ppsx
... 100.0mL: FeSO 4 ·7H 2 O 0.2g Preparation of FeSO 4 ·7H 2 O Solution: Dissolve FeSO 4 ·7H 2 O in 100.0mL of distilled/deionized water. Add three drops of concentrated HCl. Mix thoroughly. Vitamin ... isolation of Gram-negative enteric bacilli from clinical and nonclinical specimens. For the isolation and identifi- cation of microorganisms from fecal specimens. For the isolation of...
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Handbook of Microbiological Media, Fourth Edition part 24 ppsx
... Aseptically add 150.0mL of sterile liver extract, 50.0mL of sterile horse blood, 10.0mL of sterile L- cysteine·HCl solution, 10.0 mL of sterile solution A, and 5.0mL of ster- ile solution B. ... final concentration of 7%. Pour into sterile Petri dishes. Use: For the isolation, cultivation, and detection of hemolytic activity of streptococci, pneumococci, and other particularl...
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Handbook of Microbiological Media, Fourth Edition part 28 ppsx
... aseptically add 27.0mL of sterile lactose solution, 10.0mL of sterile Bromthymol Blue solution, 2.0mL of sterile sodium thiosulfate solution, 1.2mL of sterile glucose so- lution, and 1.0mL of sterile maranil ... 45°–50°C. Aseptically add 10.0mL of sterile horse serum, 1.0mL of sterile ampicillin solution, 0.25mL of ster- ile urea solution, 0.1mL of sterile nystatin solution,...
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Handbook of Microbiological Media, Fourth Edition part 29 ppsx
... 25.0g Preparation of Glucose Solution: Add 25.0g of glucose to 50.0mL of distilled/deionized water. Mix thoroughly and gently heat to dissolve. Filter sterilize. Preparation of Medium: Add components, ... sterilize. Preparation of Medium: Add components, except gelatin solution and rabbit serum, to 628.0mL of glass-distilled water. Mix thoroughly. Adjust pH to 7.6–7 .65. Add 200.0...
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Handbook of Microbiological Media, Fourth Edition part 44 ppsx
... 10.0mL of medium, 0.4mL of sterile Na 2 CO 3 solution, 0.5mL of sterile glucose solution, and 0.5mL of sterile Na 2 S·9H 2 O solution. Af- ter addition of the Na 2 CO 3 solution, the pH of the ... anaerobically add 0.4mL of sterile Na 2 CO 3 solution to each tube containing 10.0mL of medium and 0.5mL of sterile cellobiose solution to each tube. pH of the medium af- ter...
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Handbook of Microbiological Media, Fourth Edition part 47 ppsx
... 100% N 2 . Preparation of Medium: Aseptically and anoxically combine 650 .0mL of solution A with 350.0mL of Clostridum sporogenes super- natant. Use: For the cultivation of Cryptoanaerobacter spp. Cryptococcus neoformans ... 167.0mL of sterile artificial seawater and 2.0mL of sterile vitamin solution. Mix thoroughly. Preparation of Medium: Aseptically combine 500.0mL of ste...
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Handbook of Microbiological Media, Fourth Edition part 51 ppsx
... anaerobically add 50.0mL of sterile NaHCO 3 solution, 20.0mL of sterile KOH solution, 20.0mL of sterile sodium fumarate solution, 20.0mL of ster- ile sodium- L-lactate solution, 10.0mL of sterile Wolfe’s ... Aseptically and anaerobically add 0.1mL of sterile Na 2 S·9H 2 O solution, 0.1mL of sterile sodium pyruvate solution, 0.1mL of sterile vitamin solution, and 0.05mL of s...
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Handbook of Microbiological Media, Fourth Edition part 78 ppsx
... toxic. Preparation of Medium: To 65. 0mL of cooled, sterile base agar, aseptically add 10.0mL of sterile yeast dialysate, 20.0mL of horse se- rum, 2.0mL of sterile penicillin solution, and 1.0mL of sterile ... to solidify. To 65. 0mL of cooled, sterile base broth, aseptically add 10.0mL of sterile yeast di- alysate, 20.0mL of horse serum, 2.0mL of sterile penicillin solu...
Ngày tải lên: 03/07/2014, 18:20