Handbook of Microbiological Media, Fourth Edition part 60 docx

... per 60. 0mL: Sheep blood, defibrinated 50.0mL Chapman tellurite solution 10.0mL Preparation of Tellurite Blood Solution: Aseptically combine 10.0mL of Chapman tellurite solution with 50.0mL of ... add 2.0mL of sterile tellurite soltuion and 50.0mL of sterile defibrinated blood. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation and maintenance of a wide v...

Ngày tải lên: 03/07/2014, 18:20

... 10.0mL of CaCl 2 solution, 10.0mL of MgSO 4 ·7H 2 O solution, 10.0mL of NaNO 3 solution, 10.0mL of K 2 HPO 4 solution, 10.0mL of KH 2 PO 4 solution, and 10.0mL of NaCl solution to distilled/deionized water ... 100.0mL: Lactic acid 60. 0g Preparation of Ammonium Lactate Solution: Dissolve lactic acid in 100.0mL of distilled/deionized water. Neutralize with NH 4 OH to pH 7....

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... determination of phenol coefficients of disinfectant prod- ucts containing cationic surface-active materials. Use according to Official Methods of Analysis of the Association of Official Analytical Chemists ... inoculation of cultures, add 0.25mL of sterile Na 2 S·9H 2 O solution to each tube containing 9.75mL of sterile basal medium. Use: For the cultivation and maintenance...

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... and anaerobically add 50.0mL of sterile cellobiose solution, 10.0mL of sterile Wolfe’s mineral solutionn, 10.0mL of sterile Wolfe’s vitamin so- lution, and 10.0mL of sterile Na 2 S·9H 2 O solution. ... pressure–121°C. Aseptically and anaerobically add 10.0mL of sterile Wolfe’s mineral solution, 10.0mL of sterile Wolfe’s vitamin so- lution, and 10.0mL of sterile Na 2 S·9H 2 O sol...

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... Aseptically add 50.0mL of sterile blood and 1.5mL of sterile water-lysed blood solution (one part blood to three parts water). Water-lysed blood may be omitted if sterile blood is partially lysed due ... Aseptically add 50.0mL of sterile blood and 1.5mL of sterile water-lysed blood solution (one part blood to three parts water). Water-lysed blood may be omitted if sterile blood is...

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... 15.0mL Preparation of Part B: Prepare a 1M solution of K 2 CO 3 and filter sterilize. Filter sterilize 25.0mL of 95% ethanol solution. Aseptically combine 20.0mL of sterile K 2 CO 3 solution and 15.0mL of ... 60% K 2 CO 3 solution. Use: For the isolation and cultivation of Clostridium kluyveri. Clostridium kluyveri Medium Composition per liter: Part A 965.0mL Part B 35....

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... Medium: To 80.0mL of cooled, sterile basal agar, aseptically add 20.0mL of sterile horse serum, 1.0mL of sterile fresh yeast extract solution, 1.0mL of sterile urea solution, 0.5mL of sterile CVA ... sterile glucose solution, 10.0mL of sterile Wolfe’s mineral solution, 10.0mL of sterile Wolfe’s vitamin solution, 10.0mL of sterile Na 2 S·9H 2 O solution, and 10.0mL of sterile...

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... of Solution C: Add NaHCO 3 to distilled/deionized water and bring volume to 14.0mL. Mix thoroughly. Sparge with 80% N 2 + 20% CO 2 . Filter sterilize. Preparation of Medium: Inject 1.0 ml of ... sterilize. Preparation of Medium: Inject 1.0 ml of solution B and 0.2 ml of solution C in each tube of solution A. Use: For the cultivation of Rhodovibrio sodomensis=Rhodospirillum...

Ngày tải lên: 03/07/2014, 18:20

... pressure–121°C. Preparation of Medium: To each tube containing 8.9mL of sterile solution A, add (using a syringe) 1.0mL of sterile solution B, 0.1mL of sterile solution C, and 0.01mL of sterile solution ... 50° 60 C. Aseptically add in the fol- lowing order: 2.0mL of sterile menadione solution, 10.0mL of sterile L-cysteine·HCl·H 2 O solution, 10.0mL of sterile dithiothreitol s...

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... 300.0mL of sterile solution B and 200.0mL of sterile solution C. Mix thoroughly. Aseptically dispense into sterile tubes or flasks. Use: For the general cultivation of a wide variety of bacteria. FSM ... emulsion. Combine 50.0mL of egg yolk emulsion and 50.0mL of 0.9% NaCl solution. Mix thoroughly. Crystal Violet Solution: Composition per 25.0mL: Crystal Violet 0.0115g Preparatio...

Ngày tải lên: 03/07/2014, 18:20