Handbook of Microbiological Media, Fourth Edition part 45 ppt

... min at 15 psi pres- sure–121°C. Cool to 45 –50°C. Preparation of Medium: Combine 1.0L of Columbia blood agar base with 9.0g of soluble starch and 10.0μg of resazurin. Mix thor- oughly. Gently ... from other coliform colonies which have a salmon to red color. As part of an additional confirmation of E. coli, the inclusion of tryptophan improves the indole reaction, thereby in...

Ngày tải lên: 03/07/2014, 18:20

... the numbers of particular species of microorganisms. Most often, mixtures of growth factors are used in microbiological media. Acid hydrolysates of casein commonly are used as sources of amino acids. ... digest of soybean meal. It has a high vitamin and a high carbohydrate content. Polypeptone™ Peptone A mixture of peptones composed of equal parts of pancreatic digest of...

Ngày tải lên: 03/07/2014, 18:20

... 5.2g Preparation of A & A FeEDTA Solution: Dissolve 5.2g of KOH in 186.0mL of distilled/deionized water. Add 20.4g of disodium EDTA·2H 2 O. Add 13.7g of FeSO4·7H2O to 364.0mL of distilled/deion- ized ... pressure–121°C. Preparation of Medium: Saturate cooled solution A under 100% CO 2 at 0.05–0.1 atm pressure for 30 min with magnetic stirring. Add 860.0mL of solution B, 5...

Ngày tải lên: 03/07/2014, 18:20

... com- bine 500.0mL of solution 1 with 500.0mL of solution 2. Nutrient Agar: Composition per liter: Agar 15.0g Pancreatic digest of gelatin 5.0g Beef extract 3.0g Preparation of Nutrient Agar: ... 0.1g Preparation of NAD Solution: Add NAD to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Add 10.0g of agar to 700.0mL...

Ngày tải lên: 03/07/2014, 18:20

... 15 min at 15 psi pressure–121°C. Preparation of Medium: Aseptically combine 500.0mL of sterile solution A, 500.0mL of sterile solution C, and 1.0mL of sterile solution B. Mix thoroughly. Pour ... Preparation of Medium: Aseptically combine 1.0L of sterile solu- tion A and 1.0mL of sterile solution B. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use: For...

Ngày tải lên: 03/07/2014, 18:20

... identification of the Bacteri- odes fragilis group. For the differentiation of Bacteroides species based on the hydrolysis of esculin and presence of catalase. After incu- bation for 48 hr, bacteria of the ... For the cultivation of a wide variety of bacteria. BAGG Broth (Buffered Azide Glucose Glycerol Broth) Composition per liter: Pancreatic digest of casein 10.0g Peptic di...

Ngày tải lên: 03/07/2014, 18:20

... liter: Agar 20.0g NaCl 5.5g Pancreatic digest of casein 5.0g Peptic digest of animal tissue 5.0g Preparation of Bordet-Gengou Agar Base: Add components to 1.0L of 1% glycerol solution. Autoclave for ... 10.0g NaCl 5.0g Pancreatic digest of casein 5.0g Peptic digest of animal tissue 5.0g Charcoal 4.0g Nicotinic acid 1.0mg Preparation of Charcoal Agar Base: Add components of cha...

Ngày tải lên: 03/07/2014, 18:20

... Cool to 45 –50°C. Preparation of Cellulose Overlay Agar: Aseptically combine 350.0mL of cooled, sterile solution A and 650.0mL of cooled, sterile solution B. Mix thoroughly. Preparation of Medium: ... 15 min at 15 psi pressure–121°C. Cool to 45 –50°C. Preparation of Stan 5 Agar: Aseptically combine 350.0mL of cooled, sterile solution A and 650.0mL of cooled, sterile solution...

Ngày tải lên: 03/07/2014, 18:20

... combine 870.0mL of sterile solution A with 1.0mL of sterile solution B, 100.0mL of sterile solution C, 10.0mL of sterile solution D, 10.0mL of sterile solution E, and 10.0mL of sterile solution ... Medium: To 1.0L of sterile solution A, add in or- der: 1.0mL of sterile solution B, 2.0mL of sterile solution C, 50.0mL of sterile solution D, 10.0mL of sterile solution E, 1....

Ngày tải lên: 03/07/2014, 18:20

... combine 870.0mL of sterile solution A with 1.0mL of sterile solution B, 100.0mL of sterile solution C, 10.0mL of sterile solution D, 10.0mL of sterile solution E, and 10.0mL of sterile solution ... 5.0mg Riboflavin 5.0mg Thiamine·HCl 5.0mg Biotin 5.0mg Folic acid 5.0mg Vitamin B 12 5.0mg Hemin 5.0mg Preparation of Solution E (Vitamin Solution): Add 1,4-naphtho- quinone and hemi...

Ngày tải lên: 03/07/2014, 18:20