Handbook of Microbiological Media, Fourth Edition part 36 pot

... isolation of various bacteria; with the addition of blood, for the cultivation of fastidious bacteria. For the cul- tivation of Bordetella pertussis for vaccine production and the mainte- nance of stock ... isolation of various bacteria; with the addition of blood, for the cultivation of fastidious bacteria. For the cul- tivation of Bordetella pertussis for vaccine productio...

Ngày tải lên: 03/07/2014, 18:20

... Acid: Filter sterilize 40.0mL of acetic acid using a teflon filter. Preparation of Ethanol: Filter sterilize 30.0mL of ethanol using a teflon filter. Preparation of Medium: Add components, except ... anaerobically add 0.1mL of sterile glucose, 0.1mL of sterile L-cysteine·HCl·H 2 O solution, and 0.1mL of sterile Na 2 S·9H 2 O solution to each tube. Mix thoroughly. Use: For th...

Ngày tải lên: 03/07/2014, 18:20

... of Acid Fuchsin Solution: Add Acid Fuchsin to 50.0mL of 5% aqueous dioxan. Mix well to dissolve. Caution: Acid Fuchsin is a potential carcinogen and care must be tak- en to avoid inhalation of ... for 15 min at 15 psi pressure–121°C. Aseptically add 0.6mL of sterile raffinose solution, 0.2mL of sterile Na 2 CO 3 solution, and 0.2mL of sterile CoCl 2 ·6H 2 O solution to each tube....

Ngày tải lên: 03/07/2014, 18:20

... of Medium: Aseptically combine 900.0mL of solu- tion A with 100.0mL of solution B. Mix thoroughly. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation and maintenance of ... elements solution 20.0mL Preparation of Base Solution: Sparge 2.0L of distilled/deionized water with 100% N 2 to remove O 2 . Add components to 2000.0mL of O 2 - free distilled/deioni...

Ngày tải lên: 03/07/2014, 18:20

... 70.0mg Na 2 MoO 4 ·2H 2 O 36. 0mg NiCl 2 ·6H 2 O 24.0mg H 3 BO 3 6.0mg CuCl 2 ·2H 2 O 2.0mg HCl (25% solution) 10.0mL Preparation of Trace Elements Solution SL-10: Add FeCl 2 ·4H 2 O to 10.0mL of HCl solution. ... to 45°–50°C. Preparation of Medium: To 1.0L of sterile Eagle’s basal medium, aseptically add 1.0L of sterile, cooled agarose solution and 100.0mL of fetal calf serum....

Ngày tải lên: 03/07/2014, 18:20

... digest of casein 10.0g Agar 10.0g Yeast extract 5.0g Glucose 5.0g K 2 HPO 4 2.0g Liver extract 100.0mL pH 7.2 ± 0.2 at 25°C Preparation of Medium: Add 1 pound of finely ground beef liver to 2.0L of ... 100.0mL of sterile vitamin B 12 solution. Mix thoroughly. Distribute into sterile tubes or flasks. Use: For the cultivation and maintenance of Synechococcus species. For the isola...

Ngày tải lên: 03/07/2014, 18:20

... 45°–50°C. Aseptically add 100.0mL of sterile asparagine solution, 100.0mL of ster- ile methionine solution, 100.0mL of sterile sorbose solution, 10.0mL of sterile yeast extract solution, 10.0mL of sterile sodium ... Nitrofurantoin Solution: Composition per 10.0mL: Sodium nitrofurantoin 0.01g Preparation of Sodium Nitrofurantoin Solution: Add sodium nitrofurantoin to distilled/dei...

Ngày tải lên: 03/07/2014, 18:20

... 7.0mL into tubes that contain meat particles (1 part meat particles to 5 parts fluid). Autoclave for 30 min at 15 psi pressure–121°C. Use: For the cultivation of numerous anaerobes, including Actinomy- ces ... into tubes that contain meat particles (1 part meat particles to 5 parts fluid). Autoclave for 30 min at 15 psi pressure– 121°C. Cool to 25°C. Aseptically add 50 µL of sterile f...

Ngày tải lên: 03/07/2014, 18:20

... pressure–121°C. Preparation of Medium: To each tube containing 8.9mL of sterile solution A, add (using a syringe) 1.0mL of sterile solution B, 0.1mL of sterile solution C, and 0.01mL of sterile solution ... anaerobically add 100.0mL of sterile phosphate solution, 100.0mL of sterile glucose so- lution, 100.0mL of sterile NaHCO 3 solution, 10.0mL of sterile L- cysteine·HCl so...

Ngày tải lên: 03/07/2014, 18:20

... 20.0g Pancreatic digest of casein 20.0g MgSO 4 ·7H 2 O 2.0g Potassium phosphate buffer (0.02M solution, pH 7.6) 500.0mL pH 7.6 ± 0.2 at 25°C Preparation of Medium: Add agar, pancreatic digest of casein, and MgSO 4 ·7H 2 O ... Two drops of sterile rabbit serum added per tube prior to solidification en- hances the recovery of Corynebacterium diphtheriae. Use: For the cultivation and m...

Ngày tải lên: 03/07/2014, 18:20