Handbook of Microbiological Media, Fourth Edition part 35 pptx

... 45°–50°C. Preparation of Cellulose Overlay Agar: Aseptically combine 350 .0mL of cooled, sterile solution A and 650.0mL of cooled, sterile solution B. Mix thoroughly. Preparation of Medium: Pour cooled, ... 15 psi pressure–121°C. Cool to 45°–50°C. Preparation of Stan 5 Agar: Aseptically combine 350 .0mL of cooled, sterile solution A and 650.0mL of cooled, sterile solution B....

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... the numbers of particular species of microorganisms. Most often, mixtures of growth factors are used in microbiological media. Acid hydrolysates of casein commonly are used as sources of amino acids. ... digest of soybean meal. It has a high vitamin and a high carbohydrate content. Polypeptone™ Peptone A mixture of peptones composed of equal parts of pancreatic digest of...

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... cells Preparation of Heat-Killed Bacterial Suspension: Inoculate a loopful of Klebsiella pneumoniae subsp. pneumoniae ATCC 27889 into 5.0mL of nutrient broth. Incubate at 35 C overnight. Transfer 0.5mL ... Transfer 0.5mL aliquots of nutrient broth with bacterial suspension to each of ten 1.0L Erlenmeyer flasks, each containing 250.0mL of nutrient broth. Incubate cultures at 35...

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... fragilis group. For the differentiation of Bacteroides species based on the hydrolysis of esculin and presence of catalase. After incu- bation for 48 hr, bacteria of the Bacteroides fragilis group ... For the cultivation of a wide variety of bacteria. BAGG Broth (Buffered Azide Glucose Glycerol Broth) Composition per liter: Pancreatic digest of casein 10.0g Peptic digest of a...

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... combine 870.0mL of sterile solution A with 1.0mL of sterile solution B, 100.0mL of sterile solution C, 10.0mL of sterile solution D, 10.0mL of sterile solution E, and 10.0mL of sterile solution ... Medium: To 1.0L of sterile solution A, add in or- der: 1.0mL of sterile solution B, 2.0mL of sterile solution C, 50.0mL of sterile solution D, 10.0mL of sterile solution E, 1....

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... combine 870.0mL of sterile solution A with 1.0mL of sterile solution B, 100.0mL of sterile solution C, 10.0mL of sterile solution D, 10.0mL of sterile solution E, and 10.0mL of sterile solution ... 5.0mg Riboflavin 5.0mg Thiamine·HCl 5.0mg Biotin 5.0mg Folic acid 5.0mg Vitamin B 12 5.0mg Hemin 5.0mg Preparation of Solution E (Vitamin Solution): Add 1,4-naphtho- quinone and hemi...

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... 1.0mL of sterile solution E, 1.0mL of sterile solution F, 10.0mL of sterile solution G, 1.0mL of sterile solution H, 6.6mL of sterile solution I, 20.0mL of sterile solution J, and 10.0mL of sterile ... 10.0mL of sterile solution D, 1.0mL of sterile solution E, 10.0mL of sterile solution F, 6.6mL of sterile solution G, 20.0mL of sterile solution H, and 10.0mL of steri...

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... To 1.0L of sterile solution A, add in the fol- lowing order: 1.0mL of sterile solution B, 2.0mL of sterile solution C, 50.0mL of sterile solution D, 10.0mL of sterile solution E, 1.0mL of ster- ile ... solution B, 100.0mL of sterile solution C, 10.0mL of sterile solution D, 10.0mL of sterile solution E, 6.6mL of sterile solution F, 20.0mL of sterile solution G, and 10.0m...

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... freshly. Preparation of Medium: To 920.0mL of cooled, sterile solution A, aseptically and anaerobically add in the following order: 1.0mL of ster- ile solution B, 50.0mL of sterile solution C, 10.0mL of sterile ... anaerobically add 10.0mL of sterile Na 2 S·9H 2 O solution and 5.0g of sterile powdered sulfur. Mix thor- oughly. Final pH of medium should be 5.5. Use: For the cul...

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... psi pressure–121°C. Cool to 45°–50°C. Aseptically add 10.0mL of sterile vitamin solution, 10.0mL of sterile glucose solution, and 1.0mL of sterile trace elments solution. Mix thor- oughly. Pour ... 20.0g Pancreatic digest of casein 5.0g Peptic digest of animal tissue 5.0g pH5.7 ± 0.2 at 25°C Source: This medium is available as a premixed powder from BD Di- agnostic Systems. Preparat...

Ngày tải lên: 03/07/2014, 18:20